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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal SERCA1 ATPase antibody. Suitable for IHC-P, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
IHC-P | WB | |
---|---|---|
Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (By similarity). Contributes to calcium sequestration involved in muscular excitation/contraction (PubMed:10914677).
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, SERCA1, SR Ca(2+)-ATPase 1, Calcium pump 1, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, ATP2A1
Rabbit Recombinant Monoclonal SERCA1 ATPase antibody. Suitable for IHC-P, WB and reacts with Human samples.
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, SERCA1, SR Ca(2+)-ATPase 1, Calcium pump 1, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, ATP2A1
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
EPR7321
Affinity purification Protein A
2.7 x 10-11 M
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
SERCA1 ATPase ensures proper calcium regulation and muscle function by facilitating the reuptake of Ca2+ ions into the sarcoplasmic reticulum following muscle contraction. It does not operate as part of a complex but plays a significant role in calcium ion translocation thereby regulating muscle contraction-relaxation cycles. This ATPase protein is directly involved in muscle physiology and its efficient function is critical for fast muscle fibers.
SERCA1 ATPase also known as sarco/endoplasmic reticulum Ca2+ ATPase 1 is an important enzyme responsible for the active transport of Ca2+ ions from the cytosol into the sarcoplasmic reticulum which is important for muscle relaxation. This protein has a molecular weight of about 110 kDa. SERCA1 ATPase predominantly expresses in fast-twitch skeletal muscle allowing these muscles to relax rapidly after contraction. The enzyme utilizes ATP to pump calcium ions which highlights its role as an ATPase protein and its functionality in maintaining calcium homeostasis.
SERCA1 ATPase is a significant component of the calcium signaling and muscle contraction pathways. In the context of muscle contraction the release and reuptake of Ca2+ ions regulated by SERCA1 ATPase are central events. The protein works closely with the ryanodine receptor (RyR) and calsequestrin which also participate in the modulation of intracellular calcium levels. Their interactions ensure precise coordination during muscle contraction and relaxation processes.
Mutations or dysregulation of SERCA1 ATPase can lead to conditions such as Brody disease and certain forms of myopathy. Brody disease is characterized by impaired muscle relaxation which directly relates to the malfunctioning of this Ca2+ ATPase. Additionally the disrupted function of SERCA1 ATPase may also involve interactions with other proteins like the ryanodine receptor which can exacerbate muscle-related symptoms and contribute to the pathophysiology of these disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labelling SERCA1 ATPase using ab133275, at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
We recommend not to boil the samples after lysis to get desired WB results
Exposure time: 10 seconds
All lanes: Western blot - Anti-SERCA1 ATPase antibody [EPR7321] (AB133275) at 1/10000 dilution
Lane 1: Human skeletal muscle lysate, boiled at 20 µg
Lane 2: Human skeletal muscle lysate, unboiled at 20 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 110 kDa
Observed band size: 100 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com