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Rabbit Recombinant Monoclonal SERCA1 ATPase antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Not recommended
Tested
Not recommended
Mouse
Not recommended
Tested
Not recommended
Rat
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (By similarity). Contributes to calcium sequestration involved in muscular excitation/contraction (PubMed:10914677).

Alternative names

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Rabbit Recombinant Monoclonal SERCA1 ATPase antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR7322

Purification technique

Affinity purification Protein A

Specificity

This antibody can not recognize SERCA1A (ATP2A1A). ab129104 recognizes SERCA1B (ATP2A1B) only.

Dissociation constant

4.5 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248302 is the carrier-free version of ab129104.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • OI-RD Scanning - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302), expandable thumbnail

    OI-RD Scanning - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302), expandable thumbnail

    Western blot - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270449).



    NLRP3 is expressed at a much low level in normal tissues (PMID: 33538177, PMID: 25524927, PMID: 20688930, PMID: 35676979, PMID: 31923221, PMID: 24166187).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    Lane1-8?180 seconds

    All lanes: Western blot - Anti-NLRP3 antibody [EPR23073-96] (AB270449) at 1/1000 dilution

    Lane 1: Raw 264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 2: Mouse thymus tissue lysate at 20 µg

    Lane 3: Mouse lung tissue lysate at 20 µg

    Lane 4: Mouse heart tissue lysate at 20 µg

    Lane 5: Mouse kidney tissue lysate at 20 µg

    Lane 6: Mouse brain tissue lysate at 20 µg

    Lane 7: Mouse placenta tissue lysate at 20 µg

    Lane 8: Mouse ovary tissue lysate at 20 µg

    Lane 9: Mouse liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 118 kDa

    Observed band size: 118 kDa

  • Western blot - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302), expandable thumbnail

    Western blot - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)

    This data was developed using ab129104, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    We recommend not to boil the samples after lysis to get desired WB results.

    Exposure time:

    Lanes 1 and 2: 180 seconds

    Lanes 3 and 4: 10 seconds

    Lanes 5 and 6: 20 seconds

    All lanes: Western blot - Anti-SERCA1 ATPase antibody [EPR7322] (AB129104) at 1/1000 dilution

    Lane 1: Human skeletal muscle lysate, boiled at 20 µg

    Lane 2: Human skeletal muscle lysate, unboiled at 20 µg

    Lane 3: Mouse skeletal muscle lysate, boiled at 20 µg

    Lane 4: Mouse skeletal muscle lysate, unboiled at 20 µg

    Lane 5: Rat skeletal muscle lysate, boiled at 20 µg

    Lane 6: Rat skeletal muscle lysate, unboiled at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 110 kDa

    Observed band size: 100 kDa

  • Western blot - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302), expandable thumbnail

    Western blot - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)

    This data was developed using ab133275, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    We recommend not to boil the samples after lysis to get desired WB results

    Exposure time: 10 seconds

    All lanes: Western blot - Anti-SERCA1 ATPase antibody [EPR7321] (AB133275) at 1/10000 dilution

    Lane 1: Human skeletal muscle lysate, boiled at 20 µg

    Lane 2: Human skeletal muscle lysate, unboiled at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 110 kDa

    Observed band size: 100 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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