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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal SERCA1 ATPase antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IP | WB | IHC-P | |
---|---|---|---|
Human | Not recommended | Tested | Not recommended |
Mouse | Not recommended | Tested | Not recommended |
Rat | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (By similarity). Contributes to calcium sequestration involved in muscular excitation/contraction (PubMed:10914677).
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, SERCA1, SR Ca(2+)-ATPase 1, Calcium pump 1, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, ATP2A1
Rabbit Recombinant Monoclonal SERCA1 ATPase antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, SERCA1, SR Ca(2+)-ATPase 1, Calcium pump 1, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, ATP2A1
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR7322
Affinity purification Protein A
This antibody can not recognize SERCA1A (ATP2A1A). ab129104 recognizes SERCA1B (ATP2A1B) only.
4.5 x 10-11 M
Blue Ice
+4°C
Do Not Freeze
ab248302 is the carrier-free version of ab129104.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270449).
NLRP3 is expressed at a much low level in normal tissues (PMID: 33538177, PMID: 25524927, PMID: 20688930, PMID: 35676979, PMID: 31923221, PMID: 24166187).
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Lane1-8?180 seconds
All lanes: Western blot - Anti-NLRP3 antibody [EPR23073-96] (AB270449) at 1/1000 dilution
Lane 1: Raw 264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: Mouse thymus tissue lysate at 20 µg
Lane 3: Mouse lung tissue lysate at 20 µg
Lane 4: Mouse heart tissue lysate at 20 µg
Lane 5: Mouse kidney tissue lysate at 20 µg
Lane 6: Mouse brain tissue lysate at 20 µg
Lane 7: Mouse placenta tissue lysate at 20 µg
Lane 8: Mouse ovary tissue lysate at 20 µg
Lane 9: Mouse liver tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 118 kDa
This data was developed using ab129104, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
We recommend not to boil the samples after lysis to get desired WB results.
Exposure time:
Lanes 1 and 2: 180 seconds
Lanes 3 and 4: 10 seconds
Lanes 5 and 6: 20 seconds
All lanes: Western blot - Anti-SERCA1 ATPase antibody [EPR7322] (AB129104) at 1/1000 dilution
Lane 1: Human skeletal muscle lysate, boiled at 20 µg
Lane 2: Human skeletal muscle lysate, unboiled at 20 µg
Lane 3: Mouse skeletal muscle lysate, boiled at 20 µg
Lane 4: Mouse skeletal muscle lysate, unboiled at 20 µg
Lane 5: Rat skeletal muscle lysate, boiled at 20 µg
Lane 6: Rat skeletal muscle lysate, unboiled at 20 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 110 kDa
Observed band size: 100 kDa
This data was developed using ab133275, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
We recommend not to boil the samples after lysis to get desired WB results
Exposure time: 10 seconds
All lanes: Western blot - Anti-SERCA1 ATPase antibody [EPR7321] (AB133275) at 1/10000 dilution
Lane 1: Human skeletal muscle lysate, boiled at 20 µg
Lane 2: Human skeletal muscle lysate, unboiled at 20 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 110 kDa
Observed band size: 100 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com