Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (AB248302)

This data was developed using ab133275, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST We recommend not to boil the samples after lysis to get desired WB results Exposure time : 10 seconds

All lanes:

Western blot - Anti-SERCA1 ATPase antibody [EPR7321] (<a href='/en-us/products/primary-antibodies/serca1-atpase-antibody-epr7321-ab133275'>ab133275</a>) at 1/10000 dilution

Lane 1:

Human skeletal muscle lysate, boiled at 20 µg

Lane 2:

Human skeletal muscle lysate, unboiled at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 110 kDa

Observed band size: 100 kDa

false

• WB

Supplier Data

Western blot - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (AB248302)

This data was developed using ab129104, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST We recommend not to boil the samples after lysis to get desired WB results. Exposure time : Lanes 1 and 2 : 180 seconds Lanes 3 and 4 : 10 seconds Lanes 5 and 6 : 20 seconds

All lanes:

Western blot - Anti-SERCA1 ATPase antibody [EPR7322] (<a href='/en-us/products/primary-antibodies/serca1-atpase-antibody-epr7322-ab129104'>ab129104</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate, boiled at 20 µg

Lane 2:

Human skeletal muscle lysate, unboiled at 20 µg

Lane 3:

Mouse skeletal muscle lysate, boiled at 20 µg

Lane 4:

Mouse skeletal muscle lysate, unboiled at 20 µg

Lane 5:

Rat skeletal muscle lysate, boiled at 20 µg

Lane 6:

Rat skeletal muscle lysate, unboiled at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 110 kDa

Observed band size: 100 kDa

false

• WB

Supplier Data

Western blot - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (AB248302)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270449).

NLRP3 is expressed at a much low level in normal tissues (PMID : 33538177, PMID : 25524927, PMID : 20688930, PMID : 35676979, PMID : 31923221, PMID : 24166187).

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Lane1-8 : 180 seconds

NLRP3 is expressed at a much low level in normal tissues (PMID : 33538177, PMID : 25524927, PMID : 20688930, PMID : 35676979, PMID : 31923221, PMID : 24166187).

All lanes:

Western blot - Anti-NLRP3 antibody [EPR23073-96] (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-epr23073-96-ab270449'>ab270449</a>) at 1/1000 dilution

Lane 1:

Raw 264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 2:

Mouse thymus tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Lane 5:

Mouse kidney tissue lysate at 20 µg

Lane 6:

Mouse brain tissue lysate at 20 µg

Lane 7:

Mouse placenta tissue lysate at 20 µg

Lane 8:

Mouse ovary tissue lysate at 20 µg

Lane 9:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 118 kDa

Observed band size: 118 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (AB248302)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.