AB255961

Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free

BOND RX™ Validated
Recombinant
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Mouse Recombinant Monoclonal SERCA1 ATPase antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, SERCA1, SR Ca(2+)-ATPase 1, Calcium pump 1, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, ATP2A1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (AB255961)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (AB255961)

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab2818).

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.117μg/ml, followed by ready to use secondary. Cytoplasmic staining on rat skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use secondary.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (AB255961)

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab2818).

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.117μg/ml, followed by ready to use secondary. Cytoplasmic staining on mouse skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use secondary.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.

Supplier Data

Western blot - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (AB255961)

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab2818).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SERCA1 ATPase antibody [IIH11] (<a href='/en-us/products/primary-antibodies/serca1-atpase-antibody-iih11-ab2818'>ab2818</a>) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

false

Exposure time: 48s

Unconjugated

Anti-SERCA1 ATPase antibody [IIH11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IIH11

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

This antibody recognizes an epitope between amino acids 199-505 of rabbit skeletal muscle ATPase, a region that is not exposed in native sarcoplasmic reticulum.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.466 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.117 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.466 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.117 µg/mL", "IHCP-species-notes": "" } } }

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Product details

ab255961 is the carrier-free version of ab2818.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SERCA1 ATPase also known as sarco/endoplasmic reticulum Ca2+ ATPase 1 is an important enzyme responsible for the active transport of Ca2+ ions from the cytosol into the sarcoplasmic reticulum which is important for muscle relaxation. This protein has a molecular weight of about 110 kDa. SERCA1 ATPase predominantly expresses in fast-twitch skeletal muscle allowing these muscles to relax rapidly after contraction. The enzyme utilizes ATP to pump calcium ions which highlights its role as an ATPase protein and its functionality in maintaining calcium homeostasis.

Biological function summary

SERCA1 ATPase ensures proper calcium regulation and muscle function by facilitating the reuptake of Ca2+ ions into the sarcoplasmic reticulum following muscle contraction. It does not operate as part of a complex but plays a significant role in calcium ion translocation thereby regulating muscle contraction-relaxation cycles. This ATPase protein is directly involved in muscle physiology and its efficient function is critical for fast muscle fibers.

Pathways

SERCA1 ATPase is a significant component of the calcium signaling and muscle contraction pathways. In the context of muscle contraction the release and reuptake of Ca2+ ions regulated by SERCA1 ATPase are central events. The protein works closely with the ryanodine receptor (RyR) and calsequestrin which also participate in the modulation of intracellular calcium levels. Their interactions ensure precise coordination during muscle contraction and relaxation processes.

Mutations or dysregulation of SERCA1 ATPase can lead to conditions such as Brody disease and certain forms of myopathy. Brody disease is characterized by impaired muscle relaxation which directly relates to the malfunctioning of this Ca2+ ATPase. Additionally the disrupted function of SERCA1 ATPase may also involve interactions with other proteins like the ryanodine receptor which can exacerbate muscle-related symptoms and contribute to the pathophysiology of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (PubMed : 10914677, PubMed : 11438520, PubMed : 15189864, PubMed : 18075584, PubMed : 23996003, PubMed : 24270570, PubMed : 29081402). Contributes to calcium sequestration involved in muscular excitation/contraction.

See full target information ATP2A1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (AB255961)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (AB255961)

Western blot - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (AB255961)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise