Anti-SERCA1 ATPase antibody [VE121G9]

11 product images

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  Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  This blot was produced using 3-8% Tris-Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab2819 was incubated overnight at 4°C at a 1μg/ml concentration. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibody at 1/20000 dilution for 1 hour at room temperature before imaging.

  All lanes: Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819) at 1 µg/mL

  Lane 1: Human Skeletal Muscle tissue lysate at 20 µg

  Lane 2: Mouse Skeletal Muscle tissue lysate at 20 µg

  Lane 3: Human Brain tissue lysate at 20 µg

  Lane 4: Mouse Brain tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/20000 dilution

  Predicted band size: 110 kDa

  Observed band size: 110 kDa

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  Immunohistochemistry (Frozen sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  IHC image of SERCA1 ATPase staining in a section of frozen normal mouse skeletal muscle performed on a Leica BOND ™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab2819, 1ug/ml for 15 mins at room temperature and then Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913, Goat anti-Mouse IgG1 at 1.5ugml was added for 15 mins at room temperature. This was detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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  Immunohistochemistry (Frozen sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  IHC image of SERCA1 ATPase staining in a section of frozen normal human skeletal muscle* performed on a Leica BOND ™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab2819, 1ug/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  IHC image of SERCA1 ATPase staining in a section of formalin-fixed paraffin-embedded normal human skeletal muscle* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2819, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  IHC image of SERCA1 ATPase staining in a section of formalin-fixed paraffin-embedded normal mouse skeletal muscle performed on a Leica BOND ™ system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2819, 1ug/ml, for 15 mins at room temperature and then Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913, Goat anti-Mouse IgG1 at 1.5ugml was added for 15 mins at room temperature. This was detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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  This image is courtesy of an anonymous customer review

  Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  Blocking Step: 5% milk for 16 hours at 22°C
  

  This image was generated from the Hybridoma version.

  All lanes: Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819) at 1/1000 dilution

  All lanes: Whole tissue lysate of human neck muscle. at 20 µg

  Secondary

  All lanes: An HRP-conjugated sheep anti-mouse polyclonal at 1/4000 dilution

  Developed using the ECL technique.

  Predicted band size: 110 kDa

  Observed band size: 110 kDa

  Exposure time: 5min

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  Negative control image: IHC image of SERCA1 ATPase staining in a section of formalin-fixed paraffin-embedded normal mouse cerebellum performed on a Leica BOND ™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2819, 1ug/ml, for 15 mins at room temperature and then Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913, Goat anti-Mouse IgG1 at 1.5ugml was added for 15 mins at room temperature. This was detected using an HRP conjugated compact polymer system DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  Negative control image: IHC image of SERCA1 ATPase staining in a section of formalin-fixed paraffin-embedded normal human cerebellum* performed on a Leica BOND ™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2819, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunohistochemistry (Frozen sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  Negative control image: IHC image of SERCA1 ATPase staining in a section of frozen normal human cerebral cortex* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab2819, 1ug/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunohistochemistry (Frozen sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  Negative control image: IHC image of SERCA1 ATPase staining in a section of frozen normal mouse cerebral cortex performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab2819, 1ug/ml for 15 mins at room temperature and then Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913, Goat anti-Mouse IgG1 at 1.5ugml was added for 15 mins at room temperature. This was detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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  Image collected and cropped by CiteAb under a CC-BY license from the publication

  Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  SERCA1 ATPase western blot using anti-SERCA1 ATPase antibody [VE121G9] ab2819. Publication image and figure legend from Olsson, K., Cheng, A. J., et al., 2015, Skelet Muscle, PubMed 26301072.

  
  

  ab2819 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab2819 please see the product overview.

  The abundance of Ca2+-handling proteins differs markedly between human muscle fibers, myotubes and myoblasts. mRNA quantities of a RyR1 and DHPR and b SERCA1 and SERCA2 in human muscle fibers myotubes and myoblasts. Representative Western blot bands for c RyR and DHPR and d SERCA1 and SERCA2. Densitometry for the protein quantity of e RyR and DHPR and f SERCA1 and SERCA2 in human muscle fibers myotubes and myoblasts. Data are presented as the mean ± SEM. Asterisk denotes P < 0.05 relative to myoblasts, and number sign denotes P < 0.05 relative to myotubes. (N/A) not applicable due to protein quantity to low for reliable measurements