AB183531

Anti-SERCA2 ATPase antibody

Name: Anti-SERCA2 ATPase antibody (ab183531) | Abcam
Brand: Abcam Limited
SKU: AB183531
Price: 535 USD
Availability: InStock

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(2 Publications)

Goat Polyclonal SERCA2 ATPase antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ATP2A2 aa 650-700.

View Alternative Names

ATP2B, ATP2A2, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, SERCA2, SR Ca(2+)-ATPase 2, Calcium pump 2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody (AB183531)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody (AB183531)

mmunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Supplier Data

Western blot - Anti-SERCA2 ATPase antibody (AB183531)

All lanes:

Western blot - Anti-SERCA2 ATPase antibody (ab183531) at 0.1 µg/mL

Lane 1:

Human heart lysate (in RIPA buffer) at 35 µg

Lane 2:

Mouse heart lysate (in RIPA buffer) at 35 µg

Lane 3:

Rat heart lysate (in RIPA buffer) at 35 µg

Predicted band size: 115 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ATP2A2 aa 650-700. The exact immunogen used to generate this antibody is proprietary information.

P16615

Specificity

ab183531 is expected to recognize all 5 reported isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab183531 was purified from goat serum by ammonium sulfate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SERCA2 ATPase also known as sarco/endoplasmic reticulum Ca²⁺-ATPase 2 or ATP2A2 is an important pump responsible for the active transport of calcium ions from the cytosol into the sarcoplasmic or endoplasmic reticulum. This protein has a molecular weight of 110 kDa. It plays an important role in maintaining calcium homeostasis in muscle cells and is expressed in various tissues but particularly in cardiac and skeletal muscle. This pump uses energy from ATP hydrolysis to move calcium ions against their concentration gradient.

Biological function summary

The proper regulation of calcium ion levels by SERCA2 ATPase is essential for muscle contraction and relaxation cycles. SERCA2 exists as a part of the complex machinery in muscle cells closely interacting with phospholamban in the cardiac muscle to regulate its activity. Efficient functioning of SERCA2 directly influences calcium ion storage in the sarcoplasmic reticulum affecting muscle physiology and performance.

Pathways

The involvement of SERCA2 ATPase in the calcium signaling pathway and excitation-contraction coupling is significant. The protein's function is intimately linked with RYR2 (ryanodine receptor 2) as they both participate in the regulation of calcium ion flow in muscle cells. This interaction is necessary for the proper release and uptake of calcium ions during muscle contraction and relaxation cycles.

SERCA2 ATPase mutations or dysregulation can lead to cardiac diseases such as heart failure and muscle disorders like Brody myopathy. Heart failure often results from impaired calcium handling due to disruptions in the interaction between SERCA2 and associated proteins like phospholamban. Abnormalities in SERCA2 expression or function can lead to either excessive storage or insufficient release of calcium contributing to the pathological state of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (PubMed : 12542527, PubMed : 16402920). Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation (PubMed : 28890335). Also modulates ER contacts with lipid droplets, mitochondria and endosomes (PubMed : 28890335). In coordination with FLVCR2 mediates heme-stimulated switching from mitochondrial ATP synthesis to thermogenesis (By similarity).. Isoform 2. Involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca(2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca(2+) signaling cascades that promote osteoclast differentiation and activation.

See full target information ATP2A2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4101 PubMed37491347

2023

Macrophage-to-endothelial cell crosstalk by the cholesterol metabolite 27HC promotes atherosclerosis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Yu,Lin Xu,Haiyan Chu,Jun Peng,Anastasia Sacharidou,Hsi-Hsien Hsieh,Ada Weinstock,Sohaib Khan,Liqian Ma,José Gabriel Barcia Durán,Jeffrey McDonald,Erik R Nelson,Sunghee Park,Donald P McDonnell,Kathryn J Moore,Lily Jun-Shen Huang,Edward A Fisher,Chieko Mineo,Linzhang Huang,Philip W Shaul

Microbiology spectrum 10:e0083022 PubMed35604158

2022

Japanese Encephalitis Virus NS4A Protein Interacts with PTEN-Induced Kinase 1 (PINK1) and Promotes Mitophagy in Infected Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anshu Agarwal,Mohd Faraz Alam,Brohmomoy Basu,Sabyasachi Pattanayak,Shailendra Asthana,Gulam Hussain Syed,Manjula Kalia,Sudhanshu Vrati

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SERCA2 ATPase antibody

Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody (AB183531)

Western blot - Anti-SERCA2 ATPase antibody (AB183531)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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