AB233159

Anti-SERCA2 ATPase antibody

Name: Anti-SERCA2 ATPase antibody (ab233159) | Abcam
Brand: Abcam Limited
SKU: AB233159

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(1 Publication)

Rabbit Polyclonal SERCA2 ATPase antibody. Suitable for WB and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP2A2 aa 300-800.

View Alternative Names

ATP2B, ATP2A2, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, SERCA2, SR Ca(2+)-ATPase 2, Calcium pump 2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase

2 Images

Supplier Data

Western blot - Anti-SERCA2 ATPase antibody (AB233159)

All lanes:

Western blot - Anti-SERCA2 ATPase antibody (ab233159) at 2 µg/mL

All lanes:

Recombinant human SERCA2 ATPase protein

Predicted band size: 115 kDa

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Supplier Data

Western blot - Anti-SERCA2 ATPase antibody (AB233159)

All lanes:

Western blot - Anti-SERCA2 ATPase antibody (ab233159) at 2 µg/mL

All lanes:

Pig cerebrum lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 115 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

applications

Immunogen

Recombinant Fragment Protein within Human ATP2A2 aa 300-800. The exact immunogen used to generate this antibody is proprietary information.

P16615

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SERCA2 ATPase also known as sarco/endoplasmic reticulum Ca²⁺-ATPase 2 or ATP2A2 is an important pump responsible for the active transport of calcium ions from the cytosol into the sarcoplasmic or endoplasmic reticulum. This protein has a molecular weight of 110 kDa. It plays an important role in maintaining calcium homeostasis in muscle cells and is expressed in various tissues but particularly in cardiac and skeletal muscle. This pump uses energy from ATP hydrolysis to move calcium ions against their concentration gradient.

Biological function summary

The proper regulation of calcium ion levels by SERCA2 ATPase is essential for muscle contraction and relaxation cycles. SERCA2 exists as a part of the complex machinery in muscle cells closely interacting with phospholamban in the cardiac muscle to regulate its activity. Efficient functioning of SERCA2 directly influences calcium ion storage in the sarcoplasmic reticulum affecting muscle physiology and performance.

Pathways

The involvement of SERCA2 ATPase in the calcium signaling pathway and excitation-contraction coupling is significant. The protein's function is intimately linked with RYR2 (ryanodine receptor 2) as they both participate in the regulation of calcium ion flow in muscle cells. This interaction is necessary for the proper release and uptake of calcium ions during muscle contraction and relaxation cycles.

SERCA2 ATPase mutations or dysregulation can lead to cardiac diseases such as heart failure and muscle disorders like Brody myopathy. Heart failure often results from impaired calcium handling due to disruptions in the interaction between SERCA2 and associated proteins like phospholamban. Abnormalities in SERCA2 expression or function can lead to either excessive storage or insufficient release of calcium contributing to the pathological state of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (PubMed : 12542527, PubMed : 16402920). Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation (PubMed : 28890335). Also modulates ER contacts with lipid droplets, mitochondria and endosomes (PubMed : 28890335). In coordination with FLVCR2 mediates heme-stimulated switching from mitochondrial ATP synthesis to thermogenesis (By similarity).. Isoform 2. Involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca(2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca(2+) signaling cascades that promote osteoclast differentiation and activation.

See full target information ATP2A2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 12:674907 PubMed34211446

2021

PCV2 Triggers PK-15 Cell Apoptosis Through the PLC-IP3R-Ca Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Wang,Chen Li,Panpan Sun,Jianli Shi,Xiaoyan Wu,Chang Liu,Zhe Peng,Hong Han,Shaojian Xu,Ying Yang,Yao Tian,Jiaxin Li,Hongbin He,Jun Li,Zhao Wang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SERCA2 ATPase antibody

Western blot - Anti-SERCA2 ATPase antibody (AB233159)

Western blot - Anti-SERCA2 ATPase antibody (AB233159)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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