AB137020

Anti-SERCA2 ATPase antibody [EPR9393]

Name: Anti-SERCA2 ATPase antibody [EPR9393] (ab137020) | Abcam
Brand: Abcam Limited
SKU: AB137020
Price: 250 USD
Availability: InStock
Rating: 4 (3 reviews)

20ul selling size
BOND RX™ Validated
RabMAb
Recombinant
What is this?

(3 Reviews)

(12 Publications)

Rabbit Recombinant Monoclonal SERCA2 ATPase antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 12 publications.

View Alternative Names

ATP2B, ATP2A2, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, SERCA2, SR Ca(2+)-ATPase 2, Calcium pump 2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling SERCA2 ATPase with ab137020 at 1/50 dilution followed by a secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed used at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in HepG2 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Flow Cytometry (Intracellular) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling SERCA2 ATPase with ab137020 at 1/200 dilution (0.25μg) following Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) used at 1/2000 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SERCA2 ATPase with ab137020 at 1/5000 (0.018 ug/ml) dilution followed by a ready to use LeciaDS9800 (BondTM Polymer Refine Detection). Counterstained with Hematoxylin.

Positive staining on mouse cerebrum.

The section was incubated with ab137020 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling SERCA2 ATPase with ab137020 at 1/5000 (0.018 ug/ml) dilution followed by a ready to use LeciaDS9800 (BondTM Polymer Refine Detection). Counterstained with Hematoxylin.

Positive staining on mouse lung cancer.

The section was incubated with ab137020 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SERCA2 ATPase with ab137020 at 1/5000 (0.018 ug/ml) dilution followed by a ready to use LeciaDS9800 (BondTM Polymer Refine Detection). Counterstained with Hematoxylin.

Positive staining on rat cerebrum.

The section was incubated with ab137020 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

Lab

Western blot - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

All lanes:

Western blot - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, boiled at 20 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, unboiled at 20 µg

Lane 3:

C6 (Rat glial tumor glial cell) whole cell lysate, boiled at 20 µg

Lane 4:

C6 (Rat glial tumor glial cell) whole cell lysate, unboiled at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, boiled at 20 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, unboiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 115 kDa

false

Exposure time: 20s

Carrier free

Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free
660 APC

APC Anti-SERCA2 ATPase antibody [EPR9393]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-SERCA2 ATPase antibody [EPR9393]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-SERCA2 ATPase antibody [EPR9393]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-SERCA2 ATPase antibody [EPR9393]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-SERCA2 ATPase antibody [EPR9393]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-SERCA2 ATPase antibody [EPR9393]
HRP

HRP Anti-SERCA2 ATPase antibody [EPR9393]
578 PE

PE Anti-SERCA2 ATPase antibody [EPR9393]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9393

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SERCA2 ATPase also known as sarco/endoplasmic reticulum Ca²⁺-ATPase 2 or ATP2A2 is an important pump responsible for the active transport of calcium ions from the cytosol into the sarcoplasmic or endoplasmic reticulum. This protein has a molecular weight of 110 kDa. It plays an important role in maintaining calcium homeostasis in muscle cells and is expressed in various tissues but particularly in cardiac and skeletal muscle. This pump uses energy from ATP hydrolysis to move calcium ions against their concentration gradient.

Biological function summary

The proper regulation of calcium ion levels by SERCA2 ATPase is essential for muscle contraction and relaxation cycles. SERCA2 exists as a part of the complex machinery in muscle cells closely interacting with phospholamban in the cardiac muscle to regulate its activity. Efficient functioning of SERCA2 directly influences calcium ion storage in the sarcoplasmic reticulum affecting muscle physiology and performance.

Pathways

The involvement of SERCA2 ATPase in the calcium signaling pathway and excitation-contraction coupling is significant. The protein's function is intimately linked with RYR2 (ryanodine receptor 2) as they both participate in the regulation of calcium ion flow in muscle cells. This interaction is necessary for the proper release and uptake of calcium ions during muscle contraction and relaxation cycles.

SERCA2 ATPase mutations or dysregulation can lead to cardiac diseases such as heart failure and muscle disorders like Brody myopathy. Heart failure often results from impaired calcium handling due to disruptions in the interaction between SERCA2 and associated proteins like phospholamban. Abnormalities in SERCA2 expression or function can lead to either excessive storage or insufficient release of calcium contributing to the pathological state of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (PubMed : 12542527, PubMed : 16402920). Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation (PubMed : 28890335). Also modulates ER contacts with lipid droplets, mitochondria and endosomes (PubMed : 28890335). In coordination with FLVCR2 mediates heme-stimulated switching from mitochondrial ATP synthesis to thermogenesis (By similarity).. Isoform 2. Involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca(2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca(2+) signaling cascades that promote osteoclast differentiation and activation.

See full target information ATP2A2

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of developmental biology 13: PubMed40407686

2025

Embryo Culture Impacts Heart Mitochondria in Male Adolescent Sheep.

Applications

Unspecified application

Species

Unspecified reactive species

Reza Amanollahi,Stacey L Holman,Ashley S Meakin,Monalisa Padhee,Kimberley J Botting-Lawford,Song Zhang,Severence M MacLaughlin,David O Kleemann,Simon K Walker,Jennifer M Kelly,Skye R Rudiger,I Caroline McMillen,Michael D Wiese,Mitchell C Lock,Janna L Morrison

Journal of cardiovascular development and disease 12: PubMed39997470

2025

Ontogeny of Fetal Cardiometabolic Pathways: The Potential Role of Cortisol and Thyroid Hormones in Driving the Transition from Preterm to Near-Term Heart Development in Sheep.

Applications

Unspecified application

Species

Unspecified reactive species

Reza Amanollahi,Stacey L Holman,Melanie R Bertossa,Ashley S Meakin,Kent L Thornburg,I Caroline McMillen,Michael D Wiese,Mitchell C Lock,Janna L Morrison

Physiological reports 12:e70012 PubMed39169429

2024

Alterations in cardiac contractile and regulatory proteins contribute to age-related cardiac dysfunction in male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Young Soo Han,Madona Pakkam,Matthew J Fogarty,Gary C Sieck,Frank V Brozovich

ESC heart failure 10:1401-1411 PubMed36772911

2023

MicroRNA-494 regulates high glucose-induced cardiomyocyte apoptosis and autophagy by PI3K/AKT/mTOR signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuwei Ning,Siqi Zhang,Zhikun Guo

Frontiers in cell and developmental biology 10:864516 PubMed35433671

2022

Cas9/AAV9-Mediated Somatic Mutagenesis Uncovered the Cell-Autonomous Role of Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 in Murine Cardiomyocyte Maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Junsen Lin,Zhan Chen,Luzi Yang,Lei Liu,Peng Yue,Yueshen Sun,Mingming Zhao,Xiaoling Guo,Xiaomin Hu,Yan Zhang,Hong Zhang,Yifei Li,Yuxuan Guo,Erdan Dong

Journal of neuroinflammation 19:26 PubMed35093118

2022

TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Na Liu,Liping Bai,Zhipeng Lu,Rou Gu,Dongdong Zhao,Fang Yan,Jie Bai

Nature communications 12:7219 PubMed34893614

2021

Acute RyR1 Ca leak enhances NADH-linked mitochondrial respiratory capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Nadège Zanou,Haikel Dridi,Steven Reiken,Tanes Imamura de Lima,Chris Donnelly,Umberto De Marchi,Manuele Ferrini,Jeremy Vidal,Leah Sittenfeld,Jerome N Feige,Pablo M Garcia-Roves,Isabel C Lopez-Mejia,Andrew R Marks,Johan Auwerx,Bengt Kayser,Nicolas Place

Evidence-based complementary and alternative medic 2020:1868974 PubMed33149749

2020

The Protective Effect of Qishen Granule on Heart Failure after Myocardial Infarction through Regulation of Calcium Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Yang,Qiyan Wang,Zifan Zeng,Qian Zhang,Fang Liu,Hong Chang,Chun Li,Wei Wang,Yong Wang

Cell death discovery 5:132 PubMed31508245

2019

ER stress-linked autophagy stabilizes apoptosis effector PERP and triggers its co-localization with SERCA2b at ER-plasma membrane junctions.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha J McDonnell,David G Spiller,Michael R H White,Ian A Prior,Luminita Paraoan

Cardiovascular research 116:1021-1031 PubMed31373602

2019

Myocardial MMP-2 contributes to SERCA2a proteolysis during cardiac ischaemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Andrej Roczkowsky,Brandon Y H Chan,Tim Y T Lee,Zabed Mahmud,Bridgette Hartley,Olivier Julien,Gareth Armanious,Howard S Young,Richard Schulz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SERCA2 ATPase antibody [EPR9393]

Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

Flow Cytometry (Intracellular) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

Western blot - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise