Rabbit Recombinant Monoclonal SERCA2 ATPase antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 9 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020), expandable thumbnail](https://content.abcam.com/products/images/serca2-atpase-antibody-epr9393-ab137020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430015.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)")
![Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020), expandable thumbnail](https://content.abcam.com/products/images/serca2-atpase-antibody-epr9393-ab137020--immunocytochemistry-immunofluorescence-img430014.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)")
![Western blot - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020), expandable thumbnail](https://content.abcam.com/products/images/serca2-atpase-antibody-epr9393-ab137020--western-blot-img430019.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)")
![Flow Cytometry (Intracellular) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020), expandable thumbnail](https://content.abcam.com/products/images/serca2-atpase-antibody-epr9393-ab137020--flow-cytometry-intracellular-img430018.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020), expandable thumbnail](https://content.abcam.com/products/images/serca2-atpase-antibody-epr9393-ab137020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430017.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (AB137020)")
Publications
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- ESC heart failure 10:1401-14112023MicroRNA-494 regulates high glucose-induced cardiomyocyte apoptosis and autophagy by PI3K/AKT/mTOR signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuwei Ning,Siqi Zhang,Zhikun GuoPubMed 36772911
- Frontiers in cell and developmental biology 10:8645162022Cas9/AAV9-Mediated Somatic Mutagenesis Uncovered the Cell-Autonomous Role of Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 in Murine Cardiomyocyte Maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunsen Lin,Zhan Chen,Luzi Yang,Lei Liu,Peng Yue,Yueshen Sun,Mingming Zhao,Xiaoling Guo,Xiaomin Hu,Yan Zhang,Hong Zhang,Yifei Li,Yuxuan Guo,Erdan DongPubMed 35433671
- Journal of neuroinflammation 19:262022TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Liu,Liping Bai,Zhipeng Lu,Rou Gu,Dongdong Zhao,Fang Yan,Jie BaiPubMed 35093118
- Nature communications 12:72192021Acute RyR1 Ca leak enhances NADH-linked mitochondrial respiratory capacity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadège Zanou,Haikel Dridi,Steven Reiken,Tanes Imamura de Lima,Chris Donnelly,Umberto De Marchi,Manuele Ferrini,Jeremy Vidal,Leah Sittenfeld,Jerome N Feige,Pablo M Garcia-Roves,Isabel C Lopez-Mejia,Andrew R Marks,Johan Auwerx,Bengt Kayser,Nicolas PlacePubMed 34893614
- Evidence-based complementary and alternative medic 2020:18689742020The Protective Effect of Qishen Granule on Heart Failure after Myocardial Infarction through Regulation of Calcium Homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaomin Yang et. al.PubMed 33149749
- Cell death discovery 5:1322019ER stress-linked autophagy stabilizes apoptosis effector PERP and triggers its co-localization with SERCA2b at ER-plasma membrane junctions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamantha J McDonnell et. al.PubMed 31508245
- Cardiovascular research 116:1021-10312019Myocardial MMP-2 contributes to SERCA2a proteolysis during cardiac ischaemia-reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrej Roczkowsky,Brandon Y H Chan,Tim Y T Lee,Zabed Mahmud,Bridgette Hartley,Olivier Julien,Gareth Armanious,Howard S Young,Richard SchulzPubMed 31373602
- Oncotarget 8:37717-377292017KCTD11 inhibits growth and metastasis of hepatocellular carcinoma through activating Hippo signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongliang Tong,Beng Yang,Heng Xiao,Chuanhui Peng,Wendi Hu,Xiaoyu Weng,Shaobing Cheng,Chengli Du,Zhen Lv,Chaofeng Ding,Lin Zhou,Haiyang Xie,Jian Wu,Shusen ZhengPubMed 28465479
- MethodsX 2:440-52015Sequential fractionation and isolation of subcellular proteins from tissue or cultured cells.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Tested | Tested |
| Mouse | Tested | Not recommended | Tested | Expected | Expected |
| Rat | Tested | Not recommended | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (PubMed:12542527, PubMed:16402920). Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation (PubMed:28890335). Also modulates ER contacts with lipid droplets, mitochondria and endosomes (PubMed:28890335). In coordination with FLVCR2 mediates heme-stimulated switching from mitochondrial ATP synthesis to thermogenesis (By similarity).Isoform 2Involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca(2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca(2+) signaling cascades that promote osteoclast differentiation and activation.
Alternative names
ATP2B, ATP2A2, ATP2B, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, SERCA2, SR Ca(2+)-ATPase 2, Calcium pump 2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Recommended products
Rabbit Recombinant Monoclonal SERCA2 ATPase antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 9 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR9393
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SERCA2 ATPase also known as sarco/endoplasmic reticulum Ca²⁺-ATPase 2 or ATP2A2 is an important pump responsible for the active transport of calcium ions from the cytosol into the sarcoplasmic or endoplasmic reticulum. This protein has a molecular weight of 110 kDa. It plays an important role in maintaining calcium homeostasis in muscle cells and is expressed in various tissues but particularly in cardiac and skeletal muscle. This pump uses energy from ATP hydrolysis to move calcium ions against their concentration gradient.
- Biological function summary
The proper regulation of calcium ion levels by SERCA2 ATPase is essential for muscle contraction and relaxation cycles. SERCA2 exists as a part of the complex machinery in muscle cells closely interacting with phospholamban in the cardiac muscle to regulate its activity. Efficient functioning of SERCA2 directly influences calcium ion storage in the sarcoplasmic reticulum affecting muscle physiology and performance.
- Pathways
The involvement of SERCA2 ATPase in the calcium signaling pathway and excitation-contraction coupling is significant. The protein's function is intimately linked with RYR2 (ryanodine receptor 2) as they both participate in the regulation of calcium ion flow in muscle cells. This interaction is necessary for the proper release and uptake of calcium ions during muscle contraction and relaxation cycles.
- Associated diseases and disorders
SERCA2 ATPase mutations or dysregulation can lead to cardiac diseases such as heart failure and muscle disorders like Brody myopathy. Heart failure often results from impaired calcium handling due to disruptions in the interaction between SERCA2 and associated proteins like phospholamban. Abnormalities in SERCA2 expression or function can lead to either excessive storage or insufficient release of calcium contributing to the pathological state of these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020), expandable thumbnail](https://content.abcam.com/products/images/serca2-atpase-antibody-epr9393-ab137020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430015.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)
Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling SERCA2 ATPase with ab137020 at 1/5000 (0.018 ug/ml) dilution followed by a ready to use LeciaDS9800 (BondTM Polymer Refine Detection). Counterstained with Hematoxylin.
Positive staining on mouse lung cancer.
The section was incubated with ab137020 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020), expandable thumbnail](https://content.abcam.com/products/images/serca2-atpase-antibody-epr9393-ab137020--immunocytochemistry-immunofluorescence-img430014.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)")
Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling SERCA2 ATPase with ab137020 at 1/50 dilution followed by a secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed used at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in HepG2 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
![Western blot - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020), expandable thumbnail](https://content.abcam.com/products/images/serca2-atpase-antibody-epr9393-ab137020--western-blot-img430019.jpg "Western blot - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)")
Western blot - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)
All lanes: Western blot - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, boiled at 20 µg
Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, unboiled at 20 µg
Lane 3: C6 (Rat glial tumor glial cell) whole cell lysate, boiled at 20 µg
Lane 4: C6 (Rat glial tumor glial cell) whole cell lysate, unboiled at 20 µg
Lane 5: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, boiled at 20 µg
Lane 6: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, unboiled at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 115 kDa
Exposure time: 20s
![Flow Cytometry (Intracellular) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020), expandable thumbnail](https://content.abcam.com/products/images/serca2-atpase-antibody-epr9393-ab137020--flow-cytometry-intracellular-img430018.jpg "Flow Cytometry (Intracellular) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)")
Flow Cytometry (Intracellular) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling SERCA2 ATPase with ab137020 at 1/200 dilution (0.25μg) following Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) used at 1/2000 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020), expandable thumbnail](https://content.abcam.com/products/images/serca2-atpase-antibody-epr9393-ab137020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430017.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SERCA2 ATPase with ab137020 at 1/5000 (0.018 ug/ml) dilution followed by a ready to use LeciaDS9800 (BondTM Polymer Refine Detection). Counterstained with Hematoxylin.
Positive staining on rat cerebrum.
The section was incubated with ab137020 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020), expandable thumbnail](https://content.abcam.com/products/images/serca2-atpase-antibody-epr9393-ab137020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430016.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SERCA2 ATPase with ab137020 at 1/5000 (0.018 ug/ml) dilution followed by a ready to use LeciaDS9800 (BondTM Polymer Refine Detection). Counterstained with Hematoxylin.
Positive staining on mouse cerebrum.
The section was incubated with ab137020 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020), expandable thumbnail](https://content.abcam.com/products/images/serca2-atpase-antibody-epr9393-ab137020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430016.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] (ab137020)")
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