Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (AB240095)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137020).

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling SERCA2 ATPase with ab137020 at 1/50 dilution followed by a secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed used at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in HepG2 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (AB240095)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137020).

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling SERCA2 ATPase with ab137020 at 1/200 dilution (0.25μg) following Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) used at 1/2000 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (AB240095)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137020).

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SERCA2 ATPase with ab137020 at 1/5000 (0.018 ug/ml) dilution followed by a ready to use LeciaDS9800 (BondTM Polymer Refine Detection). Counterstained with Hematoxylin.

Positive staining on mouse cerebrum.

The section was incubated with ab137020 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (AB240095)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137020).

Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling SERCA2 ATPase with ab137020 at 1/5000 (0.018 ug/ml) dilution followed by a ready to use LeciaDS9800 (BondTM Polymer Refine Detection). Counterstained with Hematoxylin.

Positive staining on mouse lung cancer.

The section was incubated with ab137020 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (AB240095)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137020).

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SERCA2 ATPase with ab137020 at 1/5000 (0.018 ug/ml) dilution followed by a ready to use LeciaDS9800 (BondTM Polymer Refine Detection). Counterstained with Hematoxylin.

Positive staining on rat cerebrum.

The section was incubated with ab137020 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

• WB

Lab

Western blot - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (AB240095)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137020). We recommend not Boiling the samples after lysis as boiling may cause membrane protein aggregates. ab181602 was used as a GAPDH loading control.

All lanes:

Western blot - Anti-SERCA2 ATPase antibody [EPR9393] (<a href='/en-us/products/primary-antibodies/serca2-atpase-antibody-epr9393-ab137020'>ab137020</a>)

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, boiled at 20 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, unboiled at 20 µg

Lane 3:

C6 (Rat glial tumor glial cell) whole cell lysate, boiled at 20 µg

Lane 4:

C6 (Rat glial tumor glial cell) whole cell lysate, unboiled at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, boiled at 20 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, unboiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 115 kDa

false

Exposure time: 20s