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Rabbit Recombinant Monoclonal SERCA2 ATPase antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (AB240095), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (AB240095), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (AB240095), expandable thumbnail
  • Western blot - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (AB240095), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (AB240095), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIPICC/IFFlow Cyt (Intra)
Human
Expected
Tested
Not recommended
Tested
Tested
Mouse
Tested
Tested
Not recommended
Expected
Expected
Rat
Tested
Tested
Not recommended
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

7 products for Alternative Version

Target data

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (PubMed:12542527, PubMed:16402920). Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation (PubMed:28890335). Also modulates ER contacts with lipid droplets, mitochondria and endosomes (PubMed:28890335). In coordination with FLVCR2 mediates heme-stimulated switching from mitochondrial ATP synthesis to thermogenesis (By similarity). Isoform 2. Involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca(2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca(2+) signaling cascades that promote osteoclast differentiation and activation.

Alternative names

ATP2B, ATP2A2, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, SERCA2, SR Ca(2+)-ATPase 2, Calcium pump 2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase

Recommended products

Rabbit Recombinant Monoclonal SERCA2 ATPase antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR9393
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240095 is the carrier-free version of Anti-SERCA2 ATPase antibody [EPR9393] ab137020.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SERCA2 ATPase also known as sarco/endoplasmic reticulum Ca²⁺-ATPase 2 or ATP2A2 is an important pump responsible for the active transport of calcium ions from the cytosol into the sarcoplasmic or endoplasmic reticulum. This protein has a molecular weight of 110 kDa. It plays an important role in maintaining calcium homeostasis in muscle cells and is expressed in various tissues but particularly in cardiac and skeletal muscle. This pump uses energy from ATP hydrolysis to move calcium ions against their concentration gradient.

Biological function summary

The proper regulation of calcium ion levels by SERCA2 ATPase is essential for muscle contraction and relaxation cycles. SERCA2 exists as a part of the complex machinery in muscle cells closely interacting with phospholamban in the cardiac muscle to regulate its activity. Efficient functioning of SERCA2 directly influences calcium ion storage in the sarcoplasmic reticulum affecting muscle physiology and performance.

Pathways

The involvement of SERCA2 ATPase in the calcium signaling pathway and excitation-contraction coupling is significant. The protein's function is intimately linked with RYR2 (ryanodine receptor 2) as they both participate in the regulation of calcium ion flow in muscle cells. This interaction is necessary for the proper release and uptake of calcium ions during muscle contraction and relaxation cycles.

Associated diseases and disorders

SERCA2 ATPase mutations or dysregulation can lead to cardiac diseases such as heart failure and muscle disorders like Brody myopathy. Heart failure often results from impaired calcium handling due to disruptions in the interaction between SERCA2 and associated proteins like phospholamban. Abnormalities in SERCA2 expression or function can lead to either excessive storage or insufficient release of calcium contributing to the pathological state of these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (ab240095), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (ab240095)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SERCA2 ATPase antibody [EPR9393] ab137020).

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SERCA2 ATPase with Anti-SERCA2 ATPase antibody [EPR9393] ab137020 at 1/5000 (0.018 ug/ml) dilution followed by a ready to use LeciaDS9800 (BondTM Polymer Refine Detection). Counterstained with Hematoxylin.

    Positive staining on mouse cerebrum.

    The section was incubated with Anti-SERCA2 ATPase antibody [EPR9393] ab137020 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (ab240095), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (ab240095)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SERCA2 ATPase antibody [EPR9393] ab137020).

    Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling SERCA2 ATPase with Anti-SERCA2 ATPase antibody [EPR9393] ab137020 at 1/5000 (0.018 ug/ml) dilution followed by a ready to use LeciaDS9800 (BondTM Polymer Refine Detection). Counterstained with Hematoxylin.

    Positive staining on mouse lung cancer.

    The section was incubated with Anti-SERCA2 ATPase antibody [EPR9393] ab137020 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (ab240095), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (ab240095)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SERCA2 ATPase antibody [EPR9393] ab137020).

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling SERCA2 ATPase with Anti-SERCA2 ATPase antibody [EPR9393] ab137020 at 1/50 dilution followed by a secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed used at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in HepG2 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  • Western blot - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (ab240095), expandable thumbnail

    Western blot - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (ab240095)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SERCA2 ATPase antibody [EPR9393] ab137020).

    We recommend not Boiling the samples after lysis as boiling may cause membrane protein aggregates.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

    All lanes: Western blot - Anti-SERCA2 ATPase antibody [EPR9393] (Anti-SERCA2 ATPase antibody [EPR9393] ab137020)

    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, boiled at 20 µg with 5% NFDM/TBST

    Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, unboiled at 20 µg with 5% NFDM/TBST

    Lane 3: C6 (Rat glial tumor glial cell) whole cell lysate, boiled at 20 µg with 5% NFDM/TBST

    Lane 4: C6 (Rat glial tumor glial cell) whole cell lysate, unboiled at 20 µg with 5% NFDM/TBST

    Lane 5: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, boiled at 20 µg with 5% NFDM/TBST

    Lane 6: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, unboiled at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 115 kDa

    Exposure time: 20s

  • Flow Cytometry (Intracellular) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (ab240095), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (ab240095)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SERCA2 ATPase antibody [EPR9393] ab137020).

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling SERCA2 ATPase with Anti-SERCA2 ATPase antibody [EPR9393] ab137020 at 1/200 dilution (0.25μg) following Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) used at 1/2000 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (ab240095), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9393] - BSA and Azide free (ab240095)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SERCA2 ATPase antibody [EPR9393] ab137020).

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SERCA2 ATPase with Anti-SERCA2 ATPase antibody [EPR9393] ab137020 at 1/5000 (0.018 ug/ml) dilution followed by a ready to use LeciaDS9800 (BondTM Polymer Refine Detection). Counterstained with Hematoxylin.

    Positive staining on rat cerebrum.

    The section was incubated with Anti-SERCA2 ATPase antibody [EPR9393] ab137020 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

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Product protocols

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