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AB154259

Anti-SERCA3 ATPase antibody - N-terminal

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(2 Publications)

Rabbit Polyclonal SERCA3 ATPase antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 aa 1-200.

View Alternative Names

Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, SERCA3, SR Ca(2+)-ATPase 3, Calcium pump 3, ATP2A3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA3 ATPase antibody - N-terminal (AB154259)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA3 ATPase antibody - N-terminal (AB154259)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling SERCA3 ATPase with ab154259 at 1/500 dilution.

Western blot - Anti-SERCA3 ATPase antibody - N-terminal (AB154259)
  • WB

Unknown

Western blot - Anti-SERCA3 ATPase antibody - N-terminal (AB154259)

5% SDS PAGE

All lanes:

Western blot - Anti-SERCA3 ATPase antibody - N-terminal (ab154259) at 1/2000 dilution

Lane 1:

Jurkat whole cell lysate at 30 µg

Lane 2:

Raji whole cell lysate at 30 µg

Lane 3:

K562 whole cell lysate at 30 µg

Predicted band size: 114 kDa

false

Western blot - Anti-SERCA3 ATPase antibody - N-terminal (AB154259)
  • WB

Unknown

Western blot - Anti-SERCA3 ATPase antibody - N-terminal (AB154259)

5% SDS PAGE

All lanes:

Western blot - Anti-SERCA3 ATPase antibody - N-terminal (ab154259) at 1/5000 dilution

All lanes:

BCL1 whole cell lysate at 30 µg

Predicted band size: 114 kDa,22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q93084

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 3 commonly known as SERCA3 ATPase plays an important role in calcium transport across the cell membrane. This protein with a mass of approximately 104 kDa facilitates the movement of calcium ions from the cytosol into the sarcoplasmic and endoplasmic reticulum relying on ATP hydrolysis for energy. SERCA3 is expressed in various tissues including endothelium and epithelial cells where it contributes to maintaining calcium homeostasis.
Biological function summary

SERCA3 ATPase influences cellular calcium signaling impacting several processes such as muscle contraction and cell proliferation. It functions as part of the ATPase family that is heavily involved in calcium ion regulation. SERCA3 works in conjunction with other calcium-binding proteins and calcium channels to ensure precise control over intracellular calcium levels which is critical for normal cellular activities.

Pathways

Several molecular mechanisms involve SERCA3 ATPase such as calcium signaling pathways and the regulation of muscle contraction process. This ATPase works closely with proteins such as calmodulin and phospholamban within these pathways to modulate calcium ion concentration and muscle function. Its efficient operation ensures the proper activation and relaxation of muscle cells highlighting its significance in specific bodily functions.

SERCA3 ATPase links to conditions like hypertension and diabetes. Both disorders involve disrupted calcium ion homeostasis which affects normal cellular and muscle activities. SERCA3 also has connections with other proteins including SERCA2 whose dysfunction or altered expression may lead to similar challenges in calcium management contributing to disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
See full target information Sarcoplasmic/endoplasmic reticulum calcium ATPase 3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 36:2484-2492 PubMed34529319

2021

LncRNA SNHG14 contributes to proinflammatory cytokine production in rheumatoid arthritis via the regulation of the miR-17-5p/MINK1-JNK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jihui Zhang,Hongwei Lei,Xiu Li

Acta biochimica et biophysica Sinica 52:207-210 PubMed31942931

2020

Enhanced store-operated calcium entry in platelets is associated with acute coronary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Guo,Jiayu Zhang,Jianhua Zhu,Qing-Hui Chen,Renjun Wang,Le Gui
View all publications
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Product promise

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