AB325007

Anti-Serglycin antibody [EPR30670-4] - BSA and Azide free (Detector)

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal Serglycin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

PRG, PRG1, SRGN, Serglycin, Hematopoietic proteoglycan core protein, Platelet proteoglycan core protein, Secretory granule proteoglycan core protein, P.PG

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Sandwich ELISA - Anti-Serglycin antibody [EPR30670-4] - BSA and Azide free (Detector) (AB325007)

sELISA

Supplier Data

Sandwich ELISA - Anti-Serglycin antibody [EPR30670-4] - BSA and Azide free (Detector) (AB325007)

Sandwich ELISA using SRGN immunogen protein standard curve with the capture antibody ab325008 at 2 µg/mL and detector antibody ab325007 at 0.5 µg/mL. Background-subtracted absorbance data values (mean +/- standard deviation are shown.)

sELISA

Supplier Data

Sandwich ELISA - Anti-Serglycin antibody [EPR30670-4] - BSA and Azide free (Detector) (AB325007)

Interpolated concentrations of native Serglycin in human control wild type HeLa (Henrietta Lacks cells) and SRGN (Serglycin) knockout HeLa cell based on 1 mg/mL extract loads. The concentrations of Serglycin were measured in duplicate and interpolated from the Serglycin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serglycin concentration was determined to be 959.4 pg/mL in wild type HeLa extract (Human wild-type HeLa cell line) and 43.0 pg/ml in SRGN (Serglycin) knockout HeLa extract (Human SRGN knockout HeLa cell line).

sELISA

Supplier Data

Sandwich ELISA - Anti-Serglycin antibody [EPR30670-4] - BSA and Azide free (Detector) (AB325007)

Interpolated concentration of native Serglycin was measured at different sample concentrations. Undiluted sample is as follows : Human Plasma-Citrate (50%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

sELISA

Supplier Data

Sandwich ELISA - Anti-Serglycin antibody [EPR30670-4] - BSA and Azide free (Detector) (AB325007)

Interpolated concentration of native Serglycin was measured at different sample concentrations. Undiluted sample is as follows : HeLa cell extract (0.5 mg/mL) and THP-1 supernatant (.4 %). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30670-4

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Clone is specific to Human and Primate Serglycin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab325007 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Serglycin. The recommended pair for sandwich ELISA is:
Capture: ab325008, Human Serglycin Capture Antibody (unconjugated)
Detector: ab325007, Human Serglycin Detector Antibody (unconjugated)
The reference range value is 4.88-312.5 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T-lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF-alpha and may also regulate protease secretion. Inhibits bone mineralization.

See full target information SRGN

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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