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AB204400

Anti-SERINC5 antibody

3

(1 Review)

|

(8 Publications)

Rabbit Polyclonal SERINC5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERINC5 aa 300-400.

View Alternative Names

C5orf12, SERINC5, Serine incorporator 5

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERINC5 antibody (AB204400)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERINC5 antibody (AB204400)

Immunohistochemical analysis of paraffin-embedded Human esophagus tissue labeling SERINC5 with ab204400 antibody at a dilution of 1/500.

Immunocytochemistry/ Immunofluorescence - Anti-SERINC5 antibody (AB204400)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SERINC5 antibody (AB204400)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling SERINC5 with ab204400 at 4 μg/mL (green).

Western blot - Anti-SERINC5 antibody (AB204400)
  • WB

Supplier Data

Western blot - Anti-SERINC5 antibody (AB204400)

All lanes:

Western blot - Anti-SERINC5 antibody (ab204400) at 1/100 dilution

Lane 1:

Molecular weight marker

Lane 2:

RT-4 cell lysate

Lane 3:

U-251 MG cell lysate

Lane 4:

Human Plasma

Lane 5:

Human liver tissue lysate

Lane 6:

Human tonsil tissue lysate

Predicted band size: 47 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SERINC5 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

Q86VE9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/250", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein SERINC5 also known as Serine Incorporator 5 or TDE1 is involved in the incorporation of serine into membrane lipids. Its molecular mass is approximately 51 kDa. SERINC5 localizes to the membrane and is primarily expressed in the brain and testes although it can be found in other tissues as well. The protein functions as a transmembrane protein interacting with other cellular components to perform its role in lipid metabolism.
Biological function summary

SERINC5 modifies the lipid composition of cellular membranes. This activity influences various cellular processes including membrane trafficking and signaling. SERINC5 does not act as a part of a larger protein complex but interacts with other proteins associated with the cellular membrane to exert its function. Through its activity SERINC5 plays a role in ensuring the proper distribution and proportion of serine-derived lipids.

Pathways

SERINC5 contributes to lipid biosynthesis and membrane biology processes. It participates in lipid metabolic pathways where its function intersects with pathways involving serine metabolism. SERINC5 is related to proteins like serine palmitoyltransferase which initiates the synthesis of sphingolipids a significant class of membrane lipids. This interaction highlights SERINC5's role in lipid homeostasis.

Changes in SERINC5 have implications for viral infections and neurological conditions. The protein influences the replication cycle of certain viruses including HIV-1 by altering membrane composition and potentially affecting viral entry or assembly. SERINC5's role in membrane integrity also connects it to neurological disorders where its interaction with proteins involved in neurotransmission might lead to altered neural functions. Understanding SERINC5’s involvement could offer insights for therapeutic strategies for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Restriction factor required to restrict infectivity of lentiviruses, such as HIV-1 : acts by inhibiting an early step of viral infection. Impairs the penetration of the viral particle into the cytoplasm (PubMed : 26416733, PubMed : 26416734). Enhances the incorporation of serine into phosphatidylserine and sphingolipids. May play a role in providing serine molecules for the formation of myelin glycosphingolipids in oligodendrocytes (By similarity).
See full target information SERINC5
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

AIDS (London, England) 39:229-240 PubMed39612239

2024

Functional variability of Nef in antagonizing SERINC5 during acute to chronic HIV-1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Weiting Li,Guoqing Li,Yuyang Liu,Lina Meng,Tianxin Zhang,Libian Wang,Haochen Li,Bin Yu,Jiaxin Wu,Chu Wang,Xianghui Yu

Journal of cellular physiology 240:e31490 PubMed39568258

2024

Serinc5 Regulates Sequential Chondrocyte Differentiation by Inhibiting Sox9 Function in Pre-Hypertrophic Chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Hata,Kanta Wakamori,Akane Hirakawa-Yamamura,Sachi Ichiyama-Kobayashi,Masaya Yamaguchi,Daisuke Okuzaki,Yoshifumi Takahata,Tomohiko Murakami,Narikazu Uzawa,Takashi Yamashiro,Riko Nishimura

mBio 13:e0292322 PubMed36409124

2022

Restriction of Influenza A Virus by SERINC5.

Applications

Unspecified application

Species

Unspecified reactive species

Kin Kui Lai,James B Munro,Guoli Shi,Saliha Majdoul,Alex A Compton,Alan Rein

Virulence 13:1720-1740 PubMed36205528

2022

Interaction of SERINC5 and IFITM1/2/3 regulates the autophagy-apoptosis-immune network under CSFV infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhui Li,Zilin Zhang,Liangliang Zhang,Qingfeng Zhou,Yuwan Li,Lin Yi,Hongxing Ding,Mingqiu Zhao,Jinding Chen,Shuangqi Fan

eLife 10: PubMed34279220

2021

Ferroptotic stress promotes the accumulation of pro-inflammatory proximal tubular cells in maladaptive renal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Shintaro Ide,Yoshihiko Kobayashi,Kana Ide,Sarah A Strausser,Koki Abe,Savannah Herbek,Lori L O'Brien,Steven D Crowley,Laura Barisoni,Aleksandra Tata,Purushothama Rao Tata,Tomokazu Souma

Frontiers in microbiology 11:580233 PubMed33013817

2020

Antiviral Role of Serine Incorporator 5 (SERINC5) Proteins in Classical Swine Fever Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhui Li,Zilin Zhang,Liangliang Zhang,Hong Li,Shuangqi Fan,Erpeng Zhu,Jindai Fan,Zhaoyao Li,Wenxian Chen,Lin Yi,Hongxing Ding,Jinding Chen,Mingqiu Zhao

mBio 11: PubMed31964738

2020

IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag.

Applications

Unspecified application

Species

Unspecified reactive species

Yadvinder S Ahi,Diborah Yimer,Guoli Shi,Saliha Majdoul,Kazi Rahman,Alan Rein,Alex A Compton

Cell reports 29:1449-1457.e5 PubMed31693887

2019

Natural HIV-1 Nef Polymorphisms Impair SERINC5 Downregulation Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Steven W Jin,Nirmin Alsahafi,Xiaomei T Kuang,Shayda A Swann,Mako Toyoda,Heinrich Göttlinger,Bruce D Walker,Takamasa Ueno,Andrés Finzi,Zabrina L Brumme,Mark A Brockman
View all publications
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