Rabbit Recombinant Monoclonal Serine Palmitoyltransferase antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
sELISA | |
---|---|
Human | Expected |
Mouse | Expected |
Rat | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases (PubMed:19416851, PubMed:19648650, PubMed:20504773, PubMed:20920666). The SPT complex is composed of SPTLC1, SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer consisting of SPTLC1 and SPTLC2/SPTLC3 forms the catalytic core (PubMed:19416851). The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference (PubMed:19416851). The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA (PubMed:19416851, PubMed:19648650). The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference (PubMed:19416851, PubMed:19648650). Crucial for adipogenesis (By similarity).
KIAA0526, LCB2, SPTLC2, Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, Long chain base biosynthesis protein 2a, Serine-palmitoyl-CoA transferase 2, LCB 2, LCB2a, SPT 2
Rabbit Recombinant Monoclonal Serine Palmitoyltransferase antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR26954-44
Affinity purification Protein A
Blue Ice
+4°C
+4°C
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This supplementary information is collated from multiple sources and compiled automatically.
Serine palmitoyltransferase (SPT) sometimes referred to as SPTLC is an enzyme that plays an important role in the initial step of sphingolipid biosynthesis. It catalyzes the condensation of serine and palmitoyl-CoA yielding 3-ketodihydrosphingosine. This enzyme complex consists of subunits SPTLC1 SPTLC2 and sometimes SPTLC3 with a molecular weight around 53 kDa for SPTLC1. Expression of SPT is seen in various tissues including the liver and nervous system supporting its critical functions across the body.
Serine palmitoyltransferase is involved in sphingolipid metabolism contributing to the formation of essential cellular components. It acts as a part of a larger enzyme complex responsible for generating sphingolipid precursors. These sphingolipids play a role in membrane structure and cell signaling affecting processes like cell growth and apoptosis. The enzyme therefore influences fundamental cellular phenomena demonstrating its widespread relevance in biochemistry and cell biology.
Serine palmitoyltransferase is vital in the de novo sphingolipid biosynthesis pathway where it initiates the creation of long-chain bases the core of most sphingolipids. It also links to the lipid metabolism pathway sharing interactions with proteins like ceramide synthase. By producing the starting materials for sphingolipids SPT integrates deeply into lipid regulation and signaling pathways affecting how cells communicate and respond to their environment.
Serine palmitoyltransferase has connections to disorders like hereditary sensory and autonomic neuropathy type I (HSAN1) and metabolic syndromes. Mutations in the SPTLC1 subunit can lead to HSAN1 illustrating its direct involvement in nerve function and health. Additionally altered sphingolipid metabolism implicates SPT in metabolic syndromes where it interacts with proteins like ceramide influencing insulin resistance and lipid homeostasis. These connections emphasize the enzyme's significance in disease development and potential therapeutic targeting.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Sandwich ELISA of Serine Palmitoyltransferase 2 Antibody Pair - BSA and Azide free ab305332 with the capture antibody dilution at 2 ?g/mL and detector antibody dilution at 0.5 ?g/mL.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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