Rabbit Recombinant Monoclonal Serine Palmitoyltransferase antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Rat, Human, Mouse samples.
Images
![Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433), expandable thumbnail](https://content.abcam.com/products/images/serine-palmitoyltransferase-antibody-epr26954-39-bsa-and-azide-free-ab307433--western-blot-img405669.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433)")
![Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433), expandable thumbnail](https://content.abcam.com/products/images/serine-palmitoyltransferase-antibody-epr26954-39-bsa-and-azide-free-ab307433--western-blot-img405668.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433)")
![Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433), expandable thumbnail](https://content.abcam.com/products/images/serine-palmitoyltransferase-antibody-epr26954-39-bsa-and-azide-free-ab307433--western-blot-img405667.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433)")
![Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433), expandable thumbnail](https://content.abcam.com/products/images/serine-palmitoyltransferase-antibody-epr26954-39-bsa-and-azide-free-ab307433--immunocytochemistry-immunofluorescence-img405666.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433)")
![Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433), expandable thumbnail](https://content.abcam.com/products/images/serine-palmitoyltransferase-antibody-epr26954-39-bsa-and-azide-free-ab307433--immunocytochemistry-immunofluorescence-img405665.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases (PubMed:19416851, PubMed:19648650, PubMed:20504773, PubMed:20920666). The SPT complex is composed of SPTLC1, SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer consisting of SPTLC1 and SPTLC2/SPTLC3 forms the catalytic core (PubMed:19416851). The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference (PubMed:19416851). The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA (PubMed:19416851, PubMed:19648650). The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference (PubMed:19416851, PubMed:19648650). Crucial for adipogenesis (By similarity).
Alternative names
KIAA0526, LCB2, SPTLC2, Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, Long chain base biosynthesis protein 2a, Serine-palmitoyl-CoA transferase 2, LCB 2, LCB2a, SPT 2
Recommended products
Rabbit Recombinant Monoclonal Serine Palmitoyltransferase antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Rat, Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26954-39
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Serine palmitoyltransferase (SPT) sometimes referred to as SPTLC is an enzyme that plays an important role in the initial step of sphingolipid biosynthesis. It catalyzes the condensation of serine and palmitoyl-CoA yielding 3-ketodihydrosphingosine. This enzyme complex consists of subunits SPTLC1 SPTLC2 and sometimes SPTLC3 with a molecular weight around 53 kDa for SPTLC1. Expression of SPT is seen in various tissues including the liver and nervous system supporting its critical functions across the body.
- Biological function summary
Serine palmitoyltransferase is involved in sphingolipid metabolism contributing to the formation of essential cellular components. It acts as a part of a larger enzyme complex responsible for generating sphingolipid precursors. These sphingolipids play a role in membrane structure and cell signaling affecting processes like cell growth and apoptosis. The enzyme therefore influences fundamental cellular phenomena demonstrating its widespread relevance in biochemistry and cell biology.
- Pathways
Serine palmitoyltransferase is vital in the de novo sphingolipid biosynthesis pathway where it initiates the creation of long-chain bases the core of most sphingolipids. It also links to the lipid metabolism pathway sharing interactions with proteins like ceramide synthase. By producing the starting materials for sphingolipids SPT integrates deeply into lipid regulation and signaling pathways affecting how cells communicate and respond to their environment.
- Associated diseases and disorders
Serine palmitoyltransferase has connections to disorders like hereditary sensory and autonomic neuropathy type I (HSAN1) and metabolic syndromes. Mutations in the SPTLC1 subunit can lead to HSAN1 illustrating its direct involvement in nerve function and health. Additionally altered sphingolipid metabolism implicates SPT in metabolic syndromes where it interacts with proteins like ceramide influencing insulin resistance and lipid homeostasis. These connections emphasize the enzyme's significance in disease development and potential therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433), expandable thumbnail](https://content.abcam.com/products/images/serine-palmitoyltransferase-antibody-epr26954-39-bsa-and-azide-free-ab307433--western-blot-img405669.jpg "Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433)")
Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433)
This data was developed using 307432, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Low expression: mouse skeletal muscle (PMID:17023427)
The expression pattern and molecular weight is consistent to what has been described in the literature (PMID:17023427)
26 seconds
Exposure time:All lanes: Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (Anti-Serine Palmitoyltransferase antibody [EPR26954-39] ab307432) at 1/1000 dilution
Lane 1: Human placenta tissue lysate 20 μg
Lane 2: Human liver tissue lysate 20 μg
Lane 3: Mouse placenta tissue lysate 20 μg
Lane 4: Mouse liver tissue lysate 20 μg
Lane 5: Mouse skeletal muscle tissue lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa
Exposure time: 26s
![Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433), expandable thumbnail](https://content.abcam.com/products/images/serine-palmitoyltransferase-antibody-epr26954-39-bsa-and-azide-free-ab307433--western-blot-img405668.jpg "Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433)")
Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433)
This data was developed using 307432, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: This antibody does not cross-react with human Serine Palmitoyltransferase 3.
5.5 seconds
Exposure time:All lanes: Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (Anti-Serine Palmitoyltransferase antibody [EPR26954-39] ab307432) at 1/1000 dilution
Lane 1: His-tagged human Serine palmitoyltransferase 2 recombinant protein 10 ng
Lane 2: His-tagged human Serine palmitoyltransferase 3 recombinant protein 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa
Exposure time: 5.5s
![Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433), expandable thumbnail](https://content.abcam.com/products/images/serine-palmitoyltransferase-antibody-epr26954-39-bsa-and-azide-free-ab307433--western-blot-img405667.jpg "Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433)")
Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433)
This data was developed using 307432, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: The molecular weight is consistent to what has been described in the literature (PMID:17023427)
59 seconds
Exposure time:All lanes: Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (Anti-Serine Palmitoyltransferase antibody [EPR26954-39] ab307432) at 1/1000 dilution
Lane 1: Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate 20 μg
Lane 2: Hela transfected with siRNA specifically targeti Serine Palmitoyltransferase whole cell lysate 20 μg
Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 4: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg
Lane 5: RAW 264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate 20 μg
Lane 6: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa
Exposure time: 59s
![Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433), expandable thumbnail](https://content.abcam.com/products/images/serine-palmitoyltransferase-antibody-epr26954-39-bsa-and-azide-free-ab307433--immunocytochemistry-immunofluorescence-img405666.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433)")
Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433)
This data was developed using Anti-Serine Palmitoyltransferase antibody [EPR26954-39] ab307432, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized PC-12 (rat adrenal gland pheochromocytoma small irregularly shaped cells) cells lABelling Serine Palmitoyltransferase with Anti-Serine Palmitoyltransferase antibody [EPR26954-39] ab307432 at 1/50 (10.64 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.![Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433), expandable thumbnail](https://content.abcam.com/products/images/serine-palmitoyltransferase-antibody-epr26954-39-bsa-and-azide-free-ab307433--immunocytochemistry-immunofluorescence-img405665.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433)")
Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (ab307433)
This data was developed using Anti-Serine Palmitoyltransferase antibody [EPR26954-39] ab307432, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized MCF7 (human breast adenocarcinoma epithelial cellЈ© cells lABelling Serine Palmitoyltransferase with Anti-Serine Palmitoyltransferase antibody [EPR26954-39] ab307432 at 1/50 (10.64 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in MCF7 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.
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