Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433)

This data was developed using ab307432, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized MCF7 (human breast adenocarcinoma epithelial cellЈ© cells lABelling Serine Palmitoyltransferase with ab307432 at 1/50 (10.64 ug/ml) dilution, followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in MCF7 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433)

This data was developed using ab307432, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized PC-12 (rat adrenal gland pheochromocytoma small irregularly shaped cells) cells lABelling Serine Palmitoyltransferase with ab307432 at 1/50 (10.64 ug/ml) dilution, followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• WB

Supplier Data

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433)

This data was developed using 307432, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Low expression : mouse skeletal muscle (PMID : 17023427) The expression pattern and molecular weight is consistent to what has been described in the literature (PMID : 17023427) 26 seconds Exposure time :

All lanes:

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (<a href='/en-us/products/primary-antibodies/serine-palmitoyltransferase-antibody-epr26954-39-ab307432'>ab307432</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate 20 μg

Lane 2:

Human liver tissue lysate 20 μg

Lane 3:

Mouse placenta tissue lysate 20 μg

Lane 4:

Mouse liver tissue lysate 20 μg

Lane 5:

Mouse skeletal muscle tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433)

This data was developed using 307432, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : The molecular weight is consistent to what has been described in the literature (PMID : 17023427) 59 seconds Exposure time :

All lanes:

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (<a href='/en-us/products/primary-antibodies/serine-palmitoyltransferase-antibody-epr26954-39-ab307432'>ab307432</a>) at 1/1000 dilution

Lane 1:

Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate 20 μg

Lane 2:

Hela transfected with siRNA specifically targeti Serine Palmitoyltransferase whole cell lysate 20 μg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 4:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg

Lane 5:

RAW 264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate 20 μg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433)

This data was developed using 307432, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : This antibody does not cross-react with human Serine Palmitoyltransferase 3. 5.5 seconds Exposure time :

All lanes:

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (<a href='/en-us/products/primary-antibodies/serine-palmitoyltransferase-antibody-epr26954-39-ab307432'>ab307432</a>) at 1/1000 dilution

Lane 1:

His-tagged human Serine palmitoyltransferase 2 recombinant protein 10 ng

Lane 2:

His-tagged human Serine palmitoyltransferase 3 recombinant protein 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 5.5s