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Rabbit Recombinant Monoclonal Serine racemase antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (AB200833), expandable thumbnail
  • Western blot - Anti-Serine racemase antibody [EPR16682] (AB200833), expandable thumbnail
  • Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (AB200833), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Human
Tested
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the synthesis of D-serine from L-serine (PubMed:20106978, PubMed:23391306, PubMed:29277459). D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine (By similarity).

Alternative names

Serine racemase, D-serine ammonia-lyase, D-serine dehydratase, L-serine ammonia-lyase, L-serine dehydratase, SRR

Recommended products

Rabbit Recombinant Monoclonal Serine racemase antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16682
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Serine racemase also known as SRR is an enzyme involved in the conversion of L-serine to D-serine a process that helps regulate neural signaling. The enzyme has a molecular mass of approximately 37 kDa and is expressed highly in the brain specifically in regions such as the hippocampus and cortex. It utilizes pyridoxal phosphate (PLP) as a cofactor to catalyze the racemization and in certain conditions it also exhibits dehydratase activity converting serine to pyruvate and ammonia.

Biological function summary

Serine racemase plays an important role in the central nervous system. D-serine the product of its action serves as a co-agonist with glutamate at the NMDA receptor which is important for synaptic plasticity and memory formation. Serine racemase also associates with proteins like PICK1 and Golga3 affecting its localization and activity within cells. The regulation of D-serine levels impacts neurotransmission and neuron viability.

Pathways

Serine racemase is integral to the serine metabolism and NMDA receptor signaling pathways. It interacts with the enzyme glycine decarboxylase which is involved in glycine metabolism demonstrating its role in amino acid regulation. Its activity connects to the glutamatergic neurotransmission pathway influencing calcium influx and downstream signaling cascades that are essential for long-term potentiation and neuroplasticity.

Associated diseases and disorders

Alterations in serine racemase activity or expression have been linked to conditions such as schizophrenia and Alzheimer's disease. In schizophrenia disruptions in D-serine levels possibly through interactions with proteins like NMDA receptor and D-amino acid oxidase can lead to impaired NMDA receptor function. In Alzheimer's disease abnormal D-serine metabolism is associated with neurodegeneration often involving proteins like amyloid-beta which exacerbates disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (ab200833), expandable thumbnail

    Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (ab200833)

    Serine racemase was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HEK293 whole cell lysate 10 μg (Input). Lane 2: ab200833 IP in HEK293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab200833 in HEK293 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds

    All lanes: Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (ab200833)

    Predicted band size: 37 kDa

    Exposure time: 30s

  • Western blot - Anti-Serine racemase antibody [EPR16682] (ab200833), expandable thumbnail

    Western blot - Anti-Serine racemase antibody [EPR16682] (ab200833)

    Blocking/Dilution Buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Serine racemase antibody [EPR16682] (ab200833) at 1/5000 dilution

    Lane 1: LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg

    Lane 2: Human fetal brain tissue lysate at 20 µg

    Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

    Lane 4: HEK-293 (Human embryonic kidney) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa

    Observed band size: 37 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (ab200833), expandable thumbnail

    Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (ab200833)

    Serine racemase was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: LNCaP whole cell lysate 10 μg (Input). Lane 2: ab200833 IP in LNCaP whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab200833 in LNCaP whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (ab200833)

    Predicted band size: 37 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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