AB182217

Anti-Serine racemase antibody [EPR18747]

Name: Anti-Serine racemase antibody [EPR18747] (ab182217) | Abcam
Brand: Abcam Limited
SKU: AB182217
Price: 530 USD
Availability: InStock
Rating: 3 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(2 Publications)

Rabbit Recombinant Monoclonal Serine racemase antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

Serine racemase, D-serine ammonia-lyase, D-serine dehydratase, L-serine ammonia-lyase, L-serine dehydratase, Srr

2 Images

Supplier Data

Immunoprecipitation - Anti-Serine racemase antibody [EPR18747] (AB182217)

Serine racemase was immunoprecipitated from 0.35 mg of mouse brain tissue lysate with ab182217 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab182217 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

Lane 1 : Mouse brain tissue lysate 10 μg (Input).

Lane 2 : ab182217 IP in mouse brain tissue lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab182217 in mouse brain tissue lysate.

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-Serine racemase antibody [EPR18747] (ab182217)

Predicted band size: 37 kDa

Observed band size: 34-36 kDa

false

Supplier Data

Western blot - Anti-Serine racemase antibody [EPR18747] (AB182217)

Blocking/Dilution buffer and concentration : 5% NFDM/TBST

The expression profile observed is consistent with the literature; heart tissue is expected to be negative for serine racemase (PMID : 10557334).

All lanes:

Western blot - Anti-Serine racemase antibody [EPR18747] (ab182217) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse liver tissue lysate at 10 µg

Lane 4:

Rat brain tissue lysate at 10 µg

Lane 5:

Rat heart tissue lysate at 10 µg

Lane 6:

Rat liver tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 37 kDa

Observed band size: 34-36 kDa

true

Exposure time: 41s

Carrier free

Anti-Serine racemase antibody [EPR18747] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18747

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serine racemase also known as SRR is an enzyme involved in the conversion of L-serine to D-serine a process that helps regulate neural signaling. The enzyme has a molecular mass of approximately 37 kDa and is expressed highly in the brain specifically in regions such as the hippocampus and cortex. It utilizes pyridoxal phosphate (PLP) as a cofactor to catalyze the racemization and in certain conditions it also exhibits dehydratase activity converting serine to pyruvate and ammonia.

Biological function summary

Serine racemase plays an important role in the central nervous system. D-serine the product of its action serves as a co-agonist with glutamate at the NMDA receptor which is important for synaptic plasticity and memory formation. Serine racemase also associates with proteins like PICK1 and Golga3 affecting its localization and activity within cells. The regulation of D-serine levels impacts neurotransmission and neuron viability.

Pathways

Serine racemase is integral to the serine metabolism and NMDA receptor signaling pathways. It interacts with the enzyme glycine decarboxylase which is involved in glycine metabolism demonstrating its role in amino acid regulation. Its activity connects to the glutamatergic neurotransmission pathway influencing calcium influx and downstream signaling cascades that are essential for long-term potentiation and neuroplasticity.

Alterations in serine racemase activity or expression have been linked to conditions such as schizophrenia and Alzheimer's disease. In schizophrenia disruptions in D-serine levels possibly through interactions with proteins like NMDA receptor and D-amino acid oxidase can lead to impaired NMDA receptor function. In Alzheimer's disease abnormal D-serine metabolism is associated with neurodegeneration often involving proteins like amyloid-beta which exacerbates disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.

See full target information Serine racemase

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:4514 PubMed38802491

2024

Dynamic movement of the Golgi unit and its glycosylation enzyme zones.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiro Harada,Masataka Kunii,Kazuo Kurokawa,Takuya Sumi,Satoshi Kanda,Yu Zhang,Satomi Nadanaka,Koichiro M Hirosawa,Kazuaki Tokunaga,Takuro Tojima,Manabu Taniguchi,Kenta Moriwaki,Shin-Ichiro Yoshimura,Miki Yamamoto-Hino,Satoshi Goto,Toyomasa Katagiri,Satoshi Kume,Mitsuko Hayashi-Nishino,Miyako Nakano,Eiji Miyoshi,Kenichi G N Suzuki,Hiroshi Kitagawa,Akihiko Nakano

Biology 11: PubMed35336764

2022

Free d-Amino Acids in Salivary Gland in Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Masanobu Yoshikawa,Takugi Kan,Kosuke Shirose,Mariko Watanabe,Mitsumasa Matsuda,Kenji Ito,Mitsuru Kawaguchi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Serine racemase antibody [EPR18747]

Immunoprecipitation - Anti-Serine racemase antibody [EPR18747] (AB182217)

Western blot - Anti-Serine racemase antibody [EPR18747] (AB182217)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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