Rabbit Recombinant Monoclonal Serine/threonine-protein kinase 4/MST-1 antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Images
![Immunoprecipitation - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (AB232551), expandable thumbnail](https://content.abcam.com/products/images/serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-bsa-and-azide-free-ab232551--immunoprecipitation-img295.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (AB232551)")
![Flow Cytometry (Intracellular) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (AB232551), expandable thumbnail](https://content.abcam.com/products/images/serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-bsa-and-azide-free-ab232551--flow-cytometry-intracellular-img27449.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (AB232551)")
![Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (AB232551), expandable thumbnail](https://content.abcam.com/products/images/serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-bsa-and-azide-free-ab232551--immunocytochemistry-immunofluorescence-img38179.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (AB232551)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (AB232551), expandable thumbnail](https://content.abcam.com/products/images/serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-bsa-and-azide-free-ab232551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138073.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (AB232551)")
Publications
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- Iranian journal of basic medical sciences 24:150-1592021Exercise alleviates cardiac remodelling in diabetic cardiomyopathy via the miR-486a-5p-Mst1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Sun,Haichang Wang,Yanhui Su,Jie Lin,Mingming Zhang,Wanrong Man,Xinglong Song,Liang Zhang,Baolin Guo,Kaikai Hao,Dongdong SunPubMed 33953853
- Annals of the New York Academy of Sciences 1484:74-892020Leukemia inhibitory factor promotes gastric cancer cell proliferation, migration, and invasion via the LIFR-Hippo-YAP pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShi-Bo Bian,Yun Yang,Wen-Quan Liang,Ke-Cheng Zhang,Lin Chen,Zhong-Tao ZhangPubMed 32827446
- Medical science monitor : international medical jo 26:e9213892020RNA Binding Protein RNPC1 Suppresses the Stemness of Human Endometrial Cancer Cells via Stabilizing MST1/2 mRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingMei Wu et. al.PubMed 32088727
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | IP | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected | Tested |
| Rat | Expected | Expected | Expected | Expected | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes We strongly recommend that customers perform an antigen retrieval step. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.
Alternative names
KRS2, MST1, STK4, Serine/threonine-protein kinase 4, Mammalian STE20-like protein kinase 1, STE20-like kinase MST1, Serine/threonine-protein kinase Krs-2, MST-1
Recommended products
Rabbit Recombinant Monoclonal Serine/threonine-protein kinase 4/MST-1 antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1465Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232551 is the carrier-free version of Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] ab51134.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Serine/threonine-protein kinase 4 (MST-1) also known as STK4 is a 52 kDa enzyme that plays an important role in cellular signaling processes. This kinase ubiquitously expresses in many tissues including liver kidney and heart. MST-1 functions as a part of a complex facilitating its catalytic activity by activating downstream substrates. The alternative name for this protein is mammalian STE20-like protein kinase 1 linking MST-1 to a family of serine/threonine kinases.
- Biological function summary
This kinase orchestrates various cellular processes mainly through regulation of apoptosis and cell stress responses. MST-1 interacts with other signaling molecules in cells forming part of larger multi-protein complexes. These interactions allow MST-1 to act as a molecular switch effectively controlling the balance between cell survival and death. The cellular localization and interaction partners help diversify its biological roles.
- Pathways
MST-1 predominantly influences the Hippo signaling pathway—a pathway significant in controlling organ size and suppressing cancer. In this pathway MST-1 phosphorylates and activates LATS1/2 kinases creating an endpoint effect on cell proliferation and apoptosis. MST-1 also participates in the oxidative stress response pathway where it shows interaction with FoxO transcription factors leading to modifications in gene expression profiles.
- Associated diseases and disorders
The dysregulation of MST-1 is linked to cancer and cardiovascular diseases. In cancer the Hippo pathway's consistent activation by MST-1 helps modulate uncontrolled cell division while in cardiovascular diseases MST-1 plays a role by driving cardiomyocyte apoptosis. MST-1’s interplay with FoxO transcription factors also highlights its contribution to these diseases by reinforcing stress response and DNA repair mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551), expandable thumbnail](https://content.abcam.com/products/images/serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-bsa-and-azide-free-ab232551--immunoprecipitation-img295.jpg "Immunoprecipitation - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551)")
Immunoprecipitation - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551)
Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] ab51134 immunoprecipitating Serine/threonine-protein kinase 4. 10μg of cell lysate was incubated with primary antibody at a dilution of 1/30 and VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at a dilution of 1/1000.
Lane 1: Jurkat (human acute T cell leukemia) whole cell lysate (10ug)
Lane 2: Jurkat (human acute T cell leukemia) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] ab51134 in Jurkat (human acute T cell leukemia) whole cell lysateThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] ab51134).
All lanes: Immunoprecipitation - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] ab51134)
Predicted band size: 55 kDa
![Flow Cytometry (Intracellular) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551), expandable thumbnail](https://content.abcam.com/products/images/serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-bsa-and-azide-free-ab232551--flow-cytometry-intracellular-img27449.jpg "Flow Cytometry (Intracellular) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551)")
Flow Cytometry (Intracellular) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551)
Intracellular Flow Cytometry analysis of HeLa cells labelling Serine/theronine-protein kinase 4 /MST-1 with purified Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] ab51134 at a dilution of 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] ab51134).
![Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551), expandable thumbnail](https://content.abcam.com/products/images/serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-bsa-and-azide-free-ab232551--immunocytochemistry-immunofluorescence-img38179.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551)")
Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551)
Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] ab51134 staining Serine/threonine-protein kinase 4 / MST-1 in Raw264.7 (mouse abelson murine leukemia virus-induced tumor) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a concentration of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (mouse mAb) (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000) were used as counterstains for primary antibody Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] ab51134 and secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
Negative control 2: Mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] ab51134).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551), expandable thumbnail](https://content.abcam.com/products/images/serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-bsa-and-azide-free-ab232551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138073.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551)
Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] ab51134 staining Serine/threonine-protein kinase 4 in human gastric carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] ab51134).
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