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AB66047

Anti-Serotonin antibody

5

(4 Reviews)

|

(102 Publications)

Anti-Serotonin antibody (ab66047) is a goat polyclonal antibody detecting Serotonin.

- Over 60 publications
- Trusted since 2008

View Alternative Names

5 hydroxytryptamine, 5HT

2 Images
Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody (AB66047)
  • IHC-Fr

CiteAb

Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody (AB66047)

Serotonin immunohistochemistry-immunofluorescence using Anti-Serotonin antibody ab66047. Publication image and figure legend from Sukumaran, S. K., Lewandowski, B. C., et al., 2017, Sci Rep, PubMed 28790351.

Expression of synaptic components in type III taste cells. Immunostaining was used to identify synaptic components PCLO and CPLX2 (red, a,d) and the type III marker 5-HT (green, b,e). Merged images (c,f) indicate that PCLO is expressed in almost all 5-HT expressing cells in addition to several non 5-HT expressing cells while CPLX2 is expressed exclusively in almost all 5-HT expressing cells. Arrows point to cells co-expressing 5-HT and PCLO or CPLX2. Scale bars : C = 20 µm, F = 40 µm.

Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody (AB66047)
  • IHC-Fr

CiteAb

Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody (AB66047)

Serotonin immunohistochemistry-immunofluorescence using Anti-Serotonin antibody ab66047. Publication image and figure legend from Widmayer, P., Partsch, V., et al., 2020, Front Physiol, PubMed 32116750.

Distinct enteroendocrine cell types in the mouse antrum express Tas2r126. (A) Numerous CgA-positive EECs (red) were found to be immunoreactive for Tas2r126 (green). (B) Cells which were double labeled for Tas2r126 (green) and ghrelin (red) were also found in the antrum. (C) Dual immunostaining showed the presence of Tas2r126 immunoreactivity (green) in serotonin-producing EC cells, identified by 5-HT (red). Yellow arrows point to co-labeled cells (merge). Asterisks mark hormone-producing cells devoid of Tas2r126 staining. Scale bars (A-C), 10 μm.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

IHC-FoFr

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IHCFoFr-species-checked": "testedAndGuaranteed", "IHCFoFr-species-dilution-info": "1/400 - 1/800", "IHCFoFr-species-notes": "<p>When using Biotin/Streptavidin-HRP Technique dilution of 1/5000 - 1/10000.</p>" } } }
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Product details

If the product is lyophilized upon delivery, dilute with phosphate buffer or Tris buffer at dilutions no higher than 1/10, aliquot and freeze at -15° C or lower. Antibody can be stored for up to six months if handled as described above.

Product Specifications
Anti-Serotonin antibody (ab66047) is a goat polyclonal antibody and is validated for use in IHC-FoFr.
Anti-Serotonin antibody (ab66047) specifically detects Serotonin (UniProt ID: ; Molecular weight: kDa) and is sold in 100 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Serotonin antibody (ab66047) has high sensitivity and specificity.
Anti-Serotonin antibody (ab66047) has been cited over 68 times in peer reviewed journals and is trusted by the scientific community.
Anti-Serotonin antibody (ab66047) has 4 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone - ab157776.

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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serotonin also known as 5-hydroxytryptamine (5-HT) is a neurotransmitter mainly involved in modulating mood emotion and behavior. It weighs approximately 176.2 Da and appears in several body tissues including the central nervous system gastrointestinal tract and blood platelets. Neurons within the raphe nuclei of the brainstem predominantly synthesize serotonin influencing various physiological processes. Its mechanical role involves binding to and activating specific serotonin receptors classified into seven different types each mediating distinct signaling pathways.
Biological function summary

Serotonin regulates multiple bodily functions beyond mood such as appetite sleep and circadian rhythm. It forms part of a complex system interacting with various receptor subtypes which include the anti-serotonergic pathways inhibiting its action. Each receptor subtype leads to different downstream effects in cells contributing to the diversity of serotonin's biological roles. For research purposes scientists often use an ELISA kit to measure serotonin levels and this can influence the ELISA test cost based on the specificity and sensitivity required.

Pathways

Serotonin participates in both the serotonergic and dopaminergic pathways illustrating its central role in neurotransmission. The serotonergic pathway is essential for transmitting nerve impulses while its interaction with the dopaminergic system significantly affects mood and reward-related behaviors. Key proteins such as tryptophan hydroxylase for serotonin synthesis and the vesicular monoamine transporter are involved within these pathways and may alter serotonin's functional availability.

Serotonin is closely linked to depression and anxiety disorders where its imbalance can lead to mood disturbances. Antidepressant treatments often target serotonin receptors or its transporters restoring its levels within synapses. Serotonin's connection to these conditions extends through its relationship with proteins like the serotonin transporter (SERT) which recycles serotonin in the synaptic cleft. Understanding these dynamics presents potential targets for interventions and anti-serotonergic agents may offer therapeutic options for managing such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Serotonin
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Publications (102)

Recent publications for all applications. Explore the full list and refine your search

Histochemistry and cell biology 163:91 PubMed40960497

2025

Increased Fos immunoreactivity in astrocytes in the raphe pallidus under hypoxia, not hypercapnia.

Applications

Unspecified application

Species

Unspecified reactive species

Kouki Kato,Risa Serizawa,Takuya Yokoyama,Nobuaki Nakamuta,Yoshio Yamamoto

Nature 645:729-736 PubMed40702192

2025

A gut sense for a microbial pattern regulates feeding.

Applications

Unspecified application

Species

Unspecified reactive species

Winston W Liu,Naama Reicher,Emily Alway,Laura E Rupprecht,Peter Weng,Chloe Schaefgen,Marguerita E Klein,Jorge A Villalobos,Carlos Puerto-Hernandez,Yolanda Graciela Kiesling Altún,Amanda Carbajal,José Alfredo Aguayo-Guerrero,Alam Coss,Atharva Sahasrabudhe,Polina Anikeeva,Alan de Araujo,Avnika Bali,Guillaume de Lartigue,Elvi Gil-Lievana,Ranier Gutierrez,Edward A Miao,John F Rawls,M Maya Kaelberer,Diego V Bohórquez

Frontiers in neuroscience 19:1603486 PubMed40589789

2025

Cell types and neuronal genetic architecture in the rat CSF-contacting nucleus and the role of 5-HT in this nucleus in mediating morphine addiction through the brain-CSF circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Li,Bin Gui,Yijun Zhang,Licai Zhang

JCI insight 10: PubMed40485586

2025

Neonatal diabetes-associated missense PDX1 variant disrupts chromatin association and protein-protein interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodun Yang,Angela Zanfardino,Riccardo Schiaffini,Jeff Ishibashi,Bareket Daniel,Matthew W Haemmerle,Novella Rapini,Alessia Piscopo,Emanuele Miraglia Del Giudice,Maria Cristina Digilio,Raffaele Iorio,Mafalda Mucciolo,Stefano Cianfarani,Dario Iafusco,Fabrizio Barbetti,Doris A Stoffers

PloS one 20:e0324136 PubMed40465703

2025

Localization and characterization of cutaneous neurogenic inflammation in acute gastric mucosal injury in rats: A possible morphological explanation for visceral sensitization?

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Yi Qi,Jin-Wen Lin,Shi-Hao Wang,Li-Li Lin,Shen Lin,Jian-Guo Chen,You-Cong Ni,Xin Du,Ling-Ling He,Xin Wu,Dong Lin

Nature neuroscience 28:1146-1159 PubMed40442499

2025

The transcriptomes, connections and development of submucosal neuron classes in the mouse small intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Khomgrit Morarach,Ziwei Liu,Sanghita Banerjee,Yanan Chen,Ashley L Harb,Joel M Kosareff,Charles R Hall,Fernando López-Redondo,Elham Jalalvand,Suad H Mohamed,Anastassia Mikhailova,David R Linden,Ulrika Marklund

eLife 13: PubMed40309958

2025

GPRC6A as a novel kokumi receptor responsible for enhanced taste preferences by ornithine.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Yamamoto,Kayoko Ueji,Haruno Mizuta,Chizuko Inui-Yamamoto,Natsuko Kumamoto,Yasuhiro Shibata,Shinya Ugawa

BMC complementary medicine and therapies 25:153 PubMed40269903

2025

Anti-central fatigue effects of myelophil in 5-HTergic hyperactivity mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Yun Kang,Dong-Cheol Baek,Jin-Seok Lee,Chang-Gue Son

Cureus 16:e75926 PubMed39830563

2025

Role of Adenosine A1 Receptor in Sleep Deprivation-Induced Neuroinflammation: Insights on Rapid Eye Movement Sleep and Fear Extinction Memory Recall in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Bhanuteja Thondala,Garima Chauhan,Harsh Pawar,Koushik Ray,Monika Sharma,Neha Yadav,Sanjeev Kumar,Krishna Kishore,Usha Panjwani

Gut microbes 17:2447834 PubMed39782002

2025

Gut-derived lactic acid enhances tryptophan to 5-hydroxytryptamine in regulation of anxiety via .

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Pan,Chenglang Qian,Shaoye Huo,Yuchen Wu,Xinyi Zhao,Yueming Ying,Boyu Wang,Hao Yang,Anaguli Yeerken,Tongyao Wang,Mengwei Fu,Lihong Wang,Yuhuan Wei,Yunhua Zhao,Chunhai Shao,Huijing Wang,Chao Zhao
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Product promise

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