AB275094

Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Serotonin transporter antibody. Carrier free. Suitable for IHC-P, IP, IHC-Fr and reacts with Mouse, Rat samples.

View Alternative Names

Htt, Sert, Slc6a4, Sodium-dependent serotonin transporter, SERT, 5HT transporter, Solute carrier family 6 member 4, 5HTT

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

This data was developed using ab254358, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Serotonin transporter with ab254358 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum (PMID 23127756). The section was incubated with ab254358 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

This data was developed using ab254358, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Serotonin transporter with ab254358 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on platelets and negative staining on hepatocytes of mouse liver (PMID : 7869097). The section was incubated with ab254358 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

This data was developed using ab254358, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Serotonin transporter with ab254358 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID 23127756). The section was incubated with ab254358 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

This data was developed using ab254358, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Serotonin transporter with ab254358 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on synapses of neurons in mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

This data was developed using ab254358, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Serotonin transporter with ab254358 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

Negative control : No staining on mouse liver (PMID : 9037532) is observed.

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Unknown

Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

This data was developed using ab254358, the same antibody clone in a different buffer formulation.

Serotonin transporter was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab254358 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254358 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10 μg

Lane 2 : ab254358 IP in mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab254358 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] (<a href='/en-us/products/primary-antibodies/serotonin-transporter-antibody-epr23530-3-ab254358'>ab254358</a>)

Predicted band size: 70 kDa

Observed band size: 74 kDa

false

Unknown

Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

This data was developed using ab254358, the same antibody clone in a different buffer formulation.

Serotonin transporter was immunoprecipitated from 0.35 mg rat brain tissue lysate with ab254358 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254358 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate 10 μg

Lane 2 : ab254358 IP in rat brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab254358 in rat brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 33 seconds.

All lanes:

Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] (<a href='/en-us/products/primary-antibodies/serotonin-transporter-antibody-epr23530-3-ab254358'>ab254358</a>)

Predicted band size: 70 kDa

Observed band size: 74 kDa

false

Unconjugated

Anti-Serotonin transporter antibody [EPR23530-3]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23530-3

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IP, IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)." } } }

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Product details

ab275094 is the carrier-free version of ab254358.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The serotonin transporter (SERT) also known as 5-HTT or SLC6A4 is an important protein that facilitates the reuptake of serotonin (5-HT) from the synaptic cleft back into the presynaptic neuron. This reuptake process terminates the serotonin signaling effectively controlling its availability in the brain. SERT is approximately 620 amino acids in length with a mass of around 70 kDa. Primarily expressed in the central nervous system it is also found in peripheral tissues. Its widespread presence highlights its critical role in regulating mood and cognition via serotonin modulation.

Biological function summary

The function of SERT impacts neurotransmitter balance significantly as it is integral to serotonin regulation. By removing serotonin from the synaptic cleft and recycling it SERT maintains neurotransmitter homeostasis. SERT is not usually part of a larger protein complex but acts independently to modulate serotonin levels. The activity of SERT affects various neurological processes contributing to mood maintenance and behavioral controls.

Pathways

Serotonin transporter influences the serotonin signaling pathway which is important for mood regulation and cognitive functions. It works alongside other proteins like monoamine oxidase which degrades serotonin and serotonin receptors that mediate its effects. Additionally SERT is involved in the pharmacological pathways of antidepressants. Escitalopram oxalate and other selective serotonin reuptake inhibitors (SSRIs) target SERT to block the reuptake of serotonin increasing its extracellular levels and synaptic availability.

SERT has a strong association with depressive disorders and anxiety due to its role in serotonin regulation. Alterations in SERT expression or function can lead to disruptions in the serotonergic system potentially resulting in mood disorders. SSRIs like escitalopram oxalate are well-known treatments that target SERT to alleviate symptoms. Moreover mutations and polymorphisms in the SERT gene have been linked to various psychiatric conditions illustrating a genetic connection to the protein’s function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serotonin transporter that cotransports serotonin with one Na(+) ion in exchange for one K(+) ion and possibly one proton in an overall electroneutral transport cycle. Transports serotonin across the plasma membrane from the extracellular compartment to the cytosol thus limiting serotonin intercellular signaling (By similarity) (PubMed : 9037532, PubMed : 9547354). Essential for serotonin homeostasis in the central nervous system. In the developing somatosensory cortex, acts in glutamatergic neurons to control serotonin uptake and its trophic functions accounting for proper spatial organization of cortical neurons and elaboration of sensory circuits. In the mature cortex, acts primarily in brainstem raphe neurons to mediate serotonin uptake from the synaptic cleft back into the pre-synaptic terminal thus terminating serotonin signaling at the synapse (PubMed : 25600870, PubMed : 9547354). Modulates mucosal serotonin levels in the gastrointestinal tract through uptake and clearance of serotonin in enterocytes. Required for enteric neurogenesis and gastrointestinal reflexes (PubMed : 27111230). Regulates blood serotonin levels by ensuring rapid high affinity uptake of serotonin from plasma to platelets, where it is further stored in dense granules via vesicular monoamine transporters and then released upon stimulation (PubMed : 18317590). Mechanistically, the transport cycle starts with an outward-open conformation having Na1(+) and Cl(-) sites occupied. The binding of a second extracellular Na2(+) ion and serotonin substrate leads to structural changes to outward-occluded to inward-occluded to inward-open, where the Na2(+) ion and serotonin are released into the cytosol. Binding of intracellular K(+) ion induces conformational transitions to inward-occluded to outward-open and completes the cycle by releasing K(+) possibly together with a proton bound to Asp-98 into the extracellular compartment. Na1(+) and Cl(-) ions remain bound throughout the transport cycle (By similarity) (PubMed : 9037532, PubMed : 9547354). Additionally, displays serotonin-induced channel-like conductance for monovalent cations, mainly Na(+) ions. The channel activity is uncoupled from the transport cycle and may contribute to the membrane resting potential or excitability (By similarity).

See full target information Slc6a4

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (AB275094)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise