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AB254839

Anti-SERP1 antibody

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(3 Publications)

Rabbit Polyclonal SERP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERP1 aa 1-50.

View Alternative Names

RAMP4, SERP1, Stress-associated endoplasmic reticulum protein 1, Ribosome-attached membrane protein 4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERP1 antibody (AB254839)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERP1 antibody (AB254839)

Paraffin-embedded human pancreas tissue stained for SERP1 using ab254839 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SERP1 antibody (AB254839)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SERP1 antibody (AB254839)

PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling SERP1 using ab254839 at 4µg/ml (green) in ICC/IF.

Western blot - Anti-SERP1 antibody (AB254839)
  • WB

Supplier Data

Western blot - Anti-SERP1 antibody (AB254839)

All lanes:

Western blot - Anti-SERP1 antibody (ab254839) at 0.4 µg/mL

Lane 1:

NIH/3T3 cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 7 kDa

false

Western blot - Anti-SERP1 antibody (AB254839)
  • WB

Supplier Data

Western blot - Anti-SERP1 antibody (AB254839)

Lane 1:

Western blot - Anti-SERP1 antibody (ab254839)

Lanes 2 - 5:

Western blot - Anti-SERP1 antibody (ab254839) at 0.4 µg/mL

Lane 1:

MW marker

Lane 2:

RT4 cell lysate

Lane 3:

U-251 MG cell lysate

Lane 4:

Human plasma

Lane 5:

Human liver tissue lysate

Predicted band size: 7 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SERP1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6X1

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XYLB antibody (AB254831)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L17 antibody (AB254850)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Stress-Associated Endoplasmic Reticulum Protein 1 (SERP1) also known as Ribosome-Associated Membrane Protein 4 (RAMP4) performs important mechanical functions within the cell. With a mass of about 24 kDa SERP1 assists in the stabilization of newly synthesized polypeptides ensuring their proper folding and maturation within the endoplasmic reticulum. This protein is ubiquitously expressed in many tissues reflecting its broad role in maintaining cellular homeostasis.
Biological function summary

The function of SERP1 involves the interaction with ribosomes and the endoplasmic reticulum's membrane-associated components. SERP1 helps manage and support the correct folding of nascent proteins especially during cellular stress conditions. It does not form part of a larger protein complex but works closely with other stress response proteins to regulate protein synthesis integrity under adverse conditions.

Pathways

Stress response mechanisms and protein synthesis serve as essential biological contexts for SERP1's function. SERP1 is active in the endoplasmic reticulum stress response pathway helping mitigate misfolded protein accumulation during conditions such as unfolded protein response (UPR). It interacts with proteins like BiP/GRP78 which plays a part in these pathways coordinating a cell's adaptation to stress.

The malfunction of SERP1 impacts neurodegenerative diseases and metabolic disorders. Research links SERP1 to conditions such as Alzheimer's disease where inefficiencies in protein handling may worsen pathology. Moreover disruptions in SERP1 activity can associate with metabolic disorders like diabetes involving proteins such as PERK which participates in managing cellular stress responses similar to SERP1.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation during ER stress. May facilitate glycosylation of target proteins after termination of ER stress. May modulate the use of N-glycosylation sites on target proteins.
See full target information SERP1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1521437 PubMed40206403

2025

Identification of disease-stage therapeutic responses of mesenchymal stromal cells retrieved from murine osteoarthritic joints.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Ivanovska,Patrizio Mancuso,Amy Burke,Conor Hennessy,Swarna Raman,Claire Dooley,Steven McLoughlin,Georgina Shaw,Eka Mukeria,Jamie Reilly,Aisling O'Brien,Thomas Ritter,Aideen E Ryan,Raj Kamath,Marc C Levesque,Deborah Van Riet,Karen English,Ian Hawthorne,Brian Johnstone,Derek W Morris,Frank Barry,J Mary Murphy

Molecular medicine reports 25: PubMed35419615

2022

SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β‑catenin/TCF/LEF signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Cai,Zhenhua Sun,Lili Zhang,Hongrui Xu

Molecular therapy oncolytics 19:163-177 PubMed33209976

2020

hsa_circ_0085539 Promotes Osteosarcoma Progression by Regulating miR-526b-5p and SERP1.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Dunwei Wang,Xu Wang,Pengcheng Liu,Ming Yan
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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