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Rabbit Recombinant Monoclonal Serpin A5 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-Serpin A5 antibody [EPR23337-69] (AB270450), expandable thumbnail
  • Immunoprecipitation - Anti-Serpin A5 antibody [EPR23337-69] (AB270450), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-69] (AB270450), expandable thumbnail
  • Western blot - Anti-Serpin A5 antibody [EPR23337-69] (AB270450), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-69] (AB270450), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and pro-inflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary-type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue- and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as a hydrophobic hormone carrier by its binding to retinoic acid.

Alternative names

PCI, PLANH3, PROCI, SERPINA5, Plasma serine protease inhibitor, Acrosomal serine protease inhibitor, Plasminogen activator inhibitor 3, Protein C inhibitor, Serpin A5, PAI-3, PAI3

Recommended products

Rabbit Recombinant Monoclonal Serpin A5 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23337-69
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Serpin A5 also known as Protein C Inhibitor (PCI) or Mesotrypsin inhibitor is a member of the serine protease inhibitor family with a mass of approximately 47 kDa. It plays a mechanical role as an inhibitor of several proteases such as trypsin tissue-type plasminogen activator and urokinase-type plasminogen activator. This protein is highly expressed in tissues including the liver kidneys and seminal plasma suggesting its involvement in reproductive and hemostatic processes.

Biological function summary

Serpin A5 regulates critical physiological processes through its inhibitory function. It interacts with and inhibits target proteases controlling pathways involved in coagulation and fibrinolysis. Serpin A5 does not form part of larger protein complexes but exerts its function through direct interactions. Its modulatory action impacts processes such as cell migration and tumor invasion highlighting its role in broader cellular environments.

Pathways

Serpin A5 is an important regulator in the coagulation cascade and fibrinolytic pathways. It acts to modulate the balance between coagulation and fibrinolysis critical for maintaining hemostasis. This protein is related to other serpins such as Antithrombin III and Plasminogen activator inhibitor-1 through its regulatory actions in these pathways. The inhibition of proteases by Serpin A5 ensures proper activation and termination of these biological processes which are essential for vascular integrity.

Associated diseases and disorders

Serpin A5 is connected to conditions like thrombophilia and prostate cancer. Dysregulation in its expression or function leads to altered coagulation increasing the risk of thrombotic events. In prostate cancer its role is linked through pathways involving the matrix metalloproteinases where Serpin A5 can influence tumor progression and metastasis. Understanding its interactions with proteins in these diseases provides insights into potential therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Serpin A5 antibody [EPR23337-69] (ab270450), expandable thumbnail

    Western blot - Anti-Serpin A5 antibody [EPR23337-69] (ab270450)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/molecular weight observed is consistent with what has been described in the literature. Bands detected between 80 kDa and 120 kDa could be either the dimeric form or serpin A5 in complex with a serine protease such as urokinase or plasma kallikrein. Bands around 50 kDa represent the intact and cleaved forms (PMID: 22206708).

    Exposure time: 8 seconds.

    All lanes: Western blot - Anti-Serpin A5 antibody [EPR23337-69] (ab270450) at 1/1000 dilution

    Lane 1: Human serum lysate at 20 µg

    Lane 2: Human plasma lysate at 20 µg

    Lane 3: Human testis tissue lysate at 20 µg

    Secondary

    All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 46 kDa

    Observed band size: 45 kDa, 52 kDa, 80-90 kDa

  • Immunoprecipitation - Anti-Serpin A5 antibody [EPR23337-69] (ab270450), expandable thumbnail

    Immunoprecipitation - Anti-Serpin A5 antibody [EPR23337-69] (ab270450)

    Serpin A5 was immunoprecipitated from 0.35 mg Human serum lysate with ab270450 at 1/30 dilution . Western blot was performed on the immunoprecipitate using ab270450 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human serum lysate 10 ug

    Lane 2: ab270450 IP in Human serum lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab270450 in Human serum lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-Serpin A5 antibody [EPR23337-69] (ab270450)

    Predicted band size: 46 kDa

    Observed band size: 52 kDa, 80-90 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-69] (ab270450), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-69] (ab270450)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Serpin A5 with ab270450 at 1/5000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the human testis (PMID: 1372913). The section was incubated with ab270450 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Serpin A5 antibody [EPR23337-69] (ab270450), expandable thumbnail

    Western blot - Anti-Serpin A5 antibody [EPR23337-69] (ab270450)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/molecular weight observed is consistent with what has been described in the literature. The band detected around 100 kDa could be either the dimeric form or serpin A5 in complex with a serine protease such as urokinase or kallikrein. Bands around 50 kDa represent the intact and cleaved forms (PMID: 22206708).

    Exposure time: Lane 1: 3 minutes; Lane 2: 37 seconds; Lane 3: 3 minutes.

    All lanes: Western blot - Anti-Serpin A5 antibody [EPR23337-69] (ab270450) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa

    Observed band size: 45 kDa, 52 kDa, 80-90 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-69] (ab270450), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-69] (ab270450)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Serpin A5 with ab270450 at 1/5000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the proximal tubular epithelium of the human kidney (PMID: 14696115). The section was incubated with ab270450 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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