Rabbit Polyclonal SERPINB1/PI2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SERPINB1.
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- Cancer immunology, immunotherapy : CII 65:575-852016Oropharyngeal squamous cell carcinomas differentially express granzyme inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPauline M W van Kempen et. al.PubMed 26993499
- Biotechnology and bioengineering 113:1902-122016Proteomic differences in recombinant CHO cells producing two similar antibody fragments.Applications:WBReactive species:Unspecified reactive speciesWolfgang Sommeregger et. al.PubMed 26913574
- PloS one 11:e01514652016Pediatric Primitive Neuroectodermal Tumors of the Central Nervous System Differentially Express Granzyme Inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeroen F Vermeulen et. al.PubMed 26963506
- American journal of physiology. Gastrointestinal a 302:G1163-702012Serpin B1 protects colonic epithelial cell via blockage of neutrophil elastase activity and its expression is enhanced in patients with ulcerative colitis.
- Oral oncology 45:771-62009Serine protease inhibitor (SERPIN) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma.Applications:IHC-P, WBReactive species:HumanMei-Yu Tseng,Shyun-Yeu Liu,Hau-Ren Chen,Yu-Jen Wu,Chien-Chih Chiu,Po-Ting Chan,Wei-Fan Chiang,Yu-Chi Liu,Chien-Yu Lu,Yuh-Shan Jou,Jeff Yi-Fu ChenPubMed 19213596
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SERPINB1. The exact immunogen used to generate this antibody is proprietary information. Database link P30740
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
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Target data
Function
Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis (PubMed:30692621). Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3 (PubMed:11747453, PubMed:30692621). Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity (PubMed:23269243). During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation (PubMed:30692621). When secreted, promotes the proliferation of beta-cells via its protease inhibitory function (PubMed:26701651).
Alternative names
ELANH2, MNEI, PI2, SERPINB1, Leukocyte elastase inhibitor, LEI, Monocyte/neutrophil elastase inhibitor, Peptidase inhibitor 2, Serpin B1, EI, M/NEI, PI-2
Recommended products
Rabbit Polyclonal SERPINB1/PI2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SERPINB1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SERPINB1. The exact immunogen used to generate this antibody is proprietary information. Database link P30740
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody is specific for SERPINB1/PI2.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SERPINB1 also known as PI2 functions as a serine protease inhibitor. It has an approximate mass of 42 kDa. Serine protease inhibitors play a role in regulating a variety of proteases to maintain cellular homeostasis. This protein mainly expresses in neutrophils epithelial cells and certain immune cells. By inhibiting neutrophil elastase and cathepsin G SERPINB1 protects tissues from protease-driven damage during inflammatory responses.
- Biological function summary
SERPINB1 acts to control proteolytic activity and ensures regulated inflammatory responses. It does not form any known complexes but interacts directly with other proteases to modulate their activity. By neutralizing serine proteases SERPINB1 helps in maintaining the balance in immune cell functions. This regulation contributes to the prevention of excessive tissue damage during inflammation emphasizing its role in immune homeostasis.
- Pathways
SERPINB1 participates in key regulatory processes such as immune response and protease inhibition pathways. It has a significant influence on pathways involving serine proteases like neutrophil elastase pathways. By interacting with these proteases SERPINB1 modulates their activities ensuring controlled inflammatory reactions and protection against tissue damage.
- Associated diseases and disorders
SERPINB1 links to conditions like chronic obstructive pulmonary disease (COPD) and acute lung injury. These conditions often involve excessive proteolytic activity and SERPINB1 acts to counterbalance this to protect the lung tissue. Additionally the protein shows interactions with other proteins like alpha-1-antitrypsin which also acts as a serine protease inhibitor. The relationship between these proteins highlights their collective role in managing protease activity associated with lung diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SERPINB1/PI2 antibody (ab47731)
All lanes: Western blot - Anti-SERPINB1/PI2 antibody (ab47731) at 1/1000 dilution
All lanes: MH-S cell lysate at 20 µL
Predicted band size: 43 kDa
Observed band size: 47 kDa, 65 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINB1/PI2 antibody (ab47731)
ab47731 (2µg/ml) staining Serpin B1 in human spleen using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining in both red and white pulp.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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