Goat Polyclonal SERPINB1/PI2 antibody. Membrane Vesicle marker. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SERPINB1 aa 100-150.
Images
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SERPINB1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P30740
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Monkey | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chimpanzee, Monkey, Gorilla | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chimpanzee, Monkey, Gorilla | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis (PubMed:30692621). Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3 (PubMed:11747453, PubMed:30692621). Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity (PubMed:23269243). During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation (PubMed:30692621). When secreted, promotes the proliferation of beta-cells via its protease inhibitory function (PubMed:26701651).
Alternative names
ELANH2, MNEI, PI2, SERPINB1, Leukocyte elastase inhibitor, LEI, Monocyte/neutrophil elastase inhibitor, Peptidase inhibitor 2, Serpin B1, EI, M/NEI, PI-2
Recommended products
Goat Polyclonal SERPINB1/PI2 antibody. Membrane Vesicle marker. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SERPINB1 aa 100-150.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SERPINB1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P30740
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SERPINB1 also known as PI2 functions as a serine protease inhibitor. It has an approximate mass of 42 kDa. Serine protease inhibitors play a role in regulating a variety of proteases to maintain cellular homeostasis. This protein mainly expresses in neutrophils epithelial cells and certain immune cells. By inhibiting neutrophil elastase and cathepsin G SERPINB1 protects tissues from protease-driven damage during inflammatory responses.
- Biological function summary
SERPINB1 acts to control proteolytic activity and ensures regulated inflammatory responses. It does not form any known complexes but interacts directly with other proteases to modulate their activity. By neutralizing serine proteases SERPINB1 helps in maintaining the balance in immune cell functions. This regulation contributes to the prevention of excessive tissue damage during inflammation emphasizing its role in immune homeostasis.
- Pathways
SERPINB1 participates in key regulatory processes such as immune response and protease inhibition pathways. It has a significant influence on pathways involving serine proteases like neutrophil elastase pathways. By interacting with these proteases SERPINB1 modulates their activities ensuring controlled inflammatory reactions and protection against tissue damage.
- Associated diseases and disorders
SERPINB1 links to conditions like chronic obstructive pulmonary disease (COPD) and acute lung injury. These conditions often involve excessive proteolytic activity and SERPINB1 acts to counterbalance this to protect the lung tissue. Additionally the protein shows interactions with other proteins like alpha-1-antitrypsin which also acts as a serine protease inhibitor. The relationship between these proteins highlights their collective role in managing protease activity associated with lung diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969)
The fainter 45 kDa band likely represents the acetylated form. Both bands are blocked by the immunizing peptide.
Primary incubation was 1 hour.
All lanes: Western blot - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969) at 0.5 µg/mL
Lane 1: Human placenta lysate (in RIPA buffer) at 35 µg
Lane 2: Human placenta lysate (in RIPA buffer) at 35 µg with immunizing peptide
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 42-45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling SERPINB1/PI2 with ab195969 at 5 μg/ml.
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