AB137588

Anti-SerpinB2/PAI-2 antibody

Name: Anti-SerpinB2/PAI-2 antibody (ab137588) | Abcam
Brand: Abcam Limited
SKU: AB137588
Price: 535 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal SerpinB2/PAI-2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERPINB2 aa 50 to C-terminus.

View Alternative Names

PAI2, PLANH2, SERPINB2, Plasminogen activator inhibitor 2, PAI-2, Monocyte Arg-serpin, Placental plasminogen activator inhibitor, Serpin B2, Urokinase inhibitor

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody (AB137588)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody (AB137588)

Immunohistochemical analysis of paraffin embedded human NCIN87 xenograft tissue labeling SerpinB2/PAI-2 with ab137588 at 1/500 dilution.

Supplier Data

Western blot - Anti-SerpinB2/PAI-2 antibody (AB137588)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-SerpinB2/PAI-2 antibody (ab137588) at 1/10000 dilution

All lanes:

U87-MG whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 47 kDa

false

Supplier Data

Western blot - Anti-SerpinB2/PAI-2 antibody (AB137588)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-SerpinB2/PAI-2 antibody (ab137588) at 1/1000 dilution

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

U87-MG whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 47 kDa

false

Supplier Data

Western blot - Anti-SerpinB2/PAI-2 antibody (AB137588)

Samples were separated by 10% SDS-PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-SerpinB2/PAI-2 antibody (ab137588) at 1/1000 dilution

All lanes:

Mouse stomach tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 47 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SERPINB2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05120

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SerpinB2 also known as PAI-2 or plasminogen activator inhibitor type 2 is a serine protease inhibitor with a molecular mass of around 60 kDa. This protein localizes mostly in the cytoplasm and occasionally gets secreted outside the cell. SerpinB2 expresses in a variety of cells including monocytes macrophages and certain epithelial cells. Its primary function involves inhibiting the urokinase plasminogen activator important for processes like fibrinolysis and tissue remodeling.

Biological function summary

SerpinB2 acts as a protector against excessive proteolysis by regulating extracellular matrix hydrolysis. While not permanently part of a complex SerpinB2 associates transiently with target proteases to modulate their activities. This regulation plays a role in cell migration and inflammation control impacting wound healing and immune responses.

Pathways

SerpinB2 integrates into the plasminogen activation system which is important for fibrinolysis and maintaining hemostatic balance. It works closely with other serpins and proteases such as PAI-1 and urokinase-type plasminogen activator (uPA) to control the conversion of plasminogen to plasmin. This balance impacts tissue repair and may influence invasive cell behavior through the regulation of pericellular proteolysis.

SerpinB2's alteration has been linked to the development of atherosclerosis and cancer. Research shows that SerpinB2 levels can influence the inflammatory environment possibly driving atherogenic plaque development. Similarly changes in SerpinB2 expression and activity relate to cancer metastasis potentially by affecting the balance of matrix degradation and rebuilding alongside proteins like PAI-1 and uPA to modulate tumor invasiveness and growth.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.

See full target information SERPINB2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Shock (Augusta, Ga.) 60:450-460 PubMed37548626

2023

EXTRACELLULAR CIRP INHIBITS NEUTROPHIL APOPTOSIS TO PROMOTE ITS AGING BY UPREGULATING SERPINB2 IN SEPSIS.

Applications

Unspecified application

Species

Unspecified reactive species

Junji Shimizu,Atsushi Murao,Monowar Aziz,Ping Wang

Acta neuropathologica communications 10:155 PubMed36309755

2022

The role of plasminogen activator inhibitor-2 in pneumococcal meningitis.

Applications

Unspecified application

Species

Unspecified reactive species

Nina C Teske,Joo-Yeon Engelen-Lee,Susanne Dyckhoff-Shen,Hans-Walter Pfister,Matthias Klein,Diederik van de Beek,Carsten K Kirschning,Uwe Koedel,Matthijs C Brouwer

Nature communications 13:2256 PubMed35474062

2022

Ebola virus VP35 hijacks the PKA-CREB1 pathway for replication and pathogenesis by AKIP1 association.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhu,Ting Gao,Yi Huang,Jing Jin,Di Wang,Leike Zhang,Yanwen Jin,Ping Li,Yong Hu,Yan Wu,Hainan Liu,Qincai Dong,Guangfei Wang,Tong Zheng,Caiwei Song,Yu Bai,Xun Zhang,Yaoning Liu,Weihong Yang,Ke Xu,Gang Zou,Lei Zhao,Ruiyuan Cao,Wu Zhong,Xianzhu Xia,Gengfu Xiao,Xuan Liu,Cheng Cao

Cells 11: PubMed35406715

2022

A Novel Role for Plasminogen Activator Inhibitor Type-2 as a Hypochlorite-Resistant Serine Protease Inhibitor and Holdase Chaperone.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan H Cater,Noralyn B Mañucat-Tan,Demi K Georgiou,Guomao Zhao,Irina A Buhimschi,Amy R Wyatt,Marie Ranson

American journal of physiology. Lung cellular and molecular physiology 322:L593-L606 PubMed35200041

2022

Fibrinogen mediates cadmium-induced macrophage activation and serves as a predictor of cadmium exposure in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fu Jun Li,Ranu Surolia,Pooja Singh,Kevin G Dsouza,Crystal T Stephens,Zheng Wang,Rui-Ming Liu,Sejong Bae,Young-Il Kim,Mohammad Athar,Mark T Dransfield,Veena B Antony

Bone 141:115673 PubMed33022456

2020

Trps1 transcription factor represses phosphate-induced expression of SerpinB2 in osteogenic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mairobys Socorro,Apurva Shinde,Hajime Yamazaki,Sana Khalid,Daisy Monier,Elia Beniash,Dobrawa Napierala

PloS one 10:e0130136 PubMed26083412

2015

SerpinB2 (PAI-2) Modulates Proteostasis via Binding Misfolded Proteins and Promotion of Cytoprotective Inclusion Formation.

Applications

CBA

Species

Mouse

Jodi A Lee,Justin J Yerbury,Natalie Farrawell,Robert F Shearer,Patrick Constantinescu,Danny M Hatters,Wayne A Schroder,Andreas Suhrbier,Mark R Wilson,Darren N Saunders,Marie Ranson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SerpinB2/PAI-2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody (AB137588)

Western blot - Anti-SerpinB2/PAI-2 antibody (AB137588)

Western blot - Anti-SerpinB2/PAI-2 antibody (AB137588)

Western blot - Anti-SerpinB2/PAI-2 antibody (AB137588)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Proteins & Peptides

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise