AB267463

Anti-SerpinB2/PAI-2 antibody [EPR22924-56]

BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal SerpinB2/PAI-2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PAI2, PLANH2, SERPINB2, Plasminogen activator inhibitor 2, PAI-2, Monocyte Arg-serpin, Placental plasminogen activator inhibitor, Serpin B2, Urokinase inhibitor

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling SerpinB2/PAI-2 with ab267463 at 1/4000 dilution (0.121 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab267463 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^®RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling SerpinB2/PAI-2 with ab267463 at 1/4000 dilution (0.121 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human skin. The section was incubated with ab267463 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with ematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Unknown

Western blot - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-3 : 8 seconds; Lane 4 : 10 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 28427146).

Negative on HeLa : (PMID : 22334683, 12944478).

All lanes:

Western blot - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (ab267463) at 1/1000 dilution

Lane 1:

U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Human placenta lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

421 Alexa Fluor® 405

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22924-56

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SerpinB2 also known as PAI-2 or plasminogen activator inhibitor type 2 is a serine protease inhibitor with a molecular mass of around 60 kDa. This protein localizes mostly in the cytoplasm and occasionally gets secreted outside the cell. SerpinB2 expresses in a variety of cells including monocytes macrophages and certain epithelial cells. Its primary function involves inhibiting the urokinase plasminogen activator important for processes like fibrinolysis and tissue remodeling.

Biological function summary

SerpinB2 acts as a protector against excessive proteolysis by regulating extracellular matrix hydrolysis. While not permanently part of a complex SerpinB2 associates transiently with target proteases to modulate their activities. This regulation plays a role in cell migration and inflammation control impacting wound healing and immune responses.

Pathways

SerpinB2 integrates into the plasminogen activation system which is important for fibrinolysis and maintaining hemostatic balance. It works closely with other serpins and proteases such as PAI-1 and urokinase-type plasminogen activator (uPA) to control the conversion of plasminogen to plasmin. This balance impacts tissue repair and may influence invasive cell behavior through the regulation of pericellular proteolysis.

SerpinB2's alteration has been linked to the development of atherosclerosis and cancer. Research shows that SerpinB2 levels can influence the inflammatory environment possibly driving atherogenic plaque development. Similarly changes in SerpinB2 expression and activity relate to cancer metastasis potentially by affecting the balance of matrix degradation and rebuilding alongside proteins like PAI-1 and uPA to modulate tumor invasiveness and growth.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.

See full target information SERPINB2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiothoracic surgery 19:141 PubMed38504347

2024

Smoking behavior associated upregulation of SERPINB12 promotes proliferation and metastasis via activating WNT signaling in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Zhen Zheng,Xiang Miao,Jing Chang,Hai Zhou,Jing-Jian Zhang,Hui-Min Mo,Qin Jia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SerpinB2/PAI-2 antibody [EPR22924-56]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463)

Western blot - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise