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Rabbit Recombinant Monoclonal SerpinB2/PAI-2 antibody. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463), expandable thumbnail
  • Western blot - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.

Alternative names

PAI2, PLANH2, SERPINB2, Plasminogen activator inhibitor 2, PAI-2, Monocyte Arg-serpin, Placental plasminogen activator inhibitor, Serpin B2, Urokinase inhibitor

Recommended products

Rabbit Recombinant Monoclonal SerpinB2/PAI-2 antibody. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22924-56
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SerpinB2 also known as PAI-2 or plasminogen activator inhibitor type 2 is a serine protease inhibitor with a molecular mass of around 60 kDa. This protein localizes mostly in the cytoplasm and occasionally gets secreted outside the cell. SerpinB2 expresses in a variety of cells including monocytes macrophages and certain epithelial cells. Its primary function involves inhibiting the urokinase plasminogen activator important for processes like fibrinolysis and tissue remodeling.

Biological function summary

SerpinB2 acts as a protector against excessive proteolysis by regulating extracellular matrix hydrolysis. While not permanently part of a complex SerpinB2 associates transiently with target proteases to modulate their activities. This regulation plays a role in cell migration and inflammation control impacting wound healing and immune responses.

Pathways

SerpinB2 integrates into the plasminogen activation system which is important for fibrinolysis and maintaining hemostatic balance. It works closely with other serpins and proteases such as PAI-1 and urokinase-type plasminogen activator (uPA) to control the conversion of plasminogen to plasmin. This balance impacts tissue repair and may influence invasive cell behavior through the regulation of pericellular proteolysis.

Associated diseases and disorders

SerpinB2's alteration has been linked to the development of atherosclerosis and cancer. Research shows that SerpinB2 levels can influence the inflammatory environment possibly driving atherogenic plaque development. Similarly changes in SerpinB2 expression and activity relate to cancer metastasis potentially by affecting the balance of matrix degradation and rebuilding alongside proteins like PAI-1 and uPA to modulate tumor invasiveness and growth.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (ab267463), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (ab267463)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling SerpinB2/PAI-2 with ab267463 at 1/4000 dilution (0.121 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human tonsil. The section was incubated with ab267463 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (ab267463), expandable thumbnail

    Western blot - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (ab267463)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 8 seconds; Lane 4: 10 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 28427146).

    Negative on HeLa: (PMID: 22334683, 12944478).

    All lanes: Western blot - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (ab267463) at 1/1000 dilution

    Lane 1: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

    Lane 2: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: Human placenta lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa

    Observed band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (ab267463), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (ab267463)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling SerpinB2/PAI-2 with ab267463 at 1/4000 dilution (0.121 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human skin. The section was incubated with ab267463 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with ematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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