Rabbit Polyclonal SerpinB3/SCCA antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERPINB3 aa 50 to C-terminus.
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Publications
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- Journal of applied oral science : revista FOB 29:e202103742021Evaluation of squamous cell carcinoma antigen 1 expression in oral squamous cell carcinoma (tumor cells and peritumoral T-lymphocytes) and verrucous carcinoma and comparison with normal oral mucosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamira Derakhshan,Arvin Poosti,Amirnader Emami Razavi,Mohammad Amin Moosavi,Nazanin Mahdavi,Fereshteh Baghaei Naieni,Kambiz Kamyab Hesari,Amirsina RahpeimaPubMed 34878006
- Dermatology (Basel, Switzerland) 238:236-24320213D-SeboSkin Model for Human ex vivo Studies of Hidradenitis Suppurativa/Acne Inversa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxiao Hou,Amir M Hossini,Georgios Nikolakis,Ottfried Balthasar,Andreas Kurtz,Christos C ZouboulisPubMed 34077928
- Journal of the European Academy of Dermatology and Venereology : JEADV 34:846-8612020Alterations in innate immunity and epithelial cell differentiation are the molecular pillars of hidradenitis suppurativa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC C Zouboulis,A Nogueira da Costa,E Makrantonaki,X X Hou,D Almansouri,J T Dudley,H Edwards,B Readhead,O Balthasar,G B E Jemec,N G Bonitsis,G Nikolakis,D Trebing,K C Zouboulis,A M HossiniPubMed 31838778
- Oncology reports 41:1253-12632018Oncogenic Ras/squamous cell carcinoma antigen signaling pathway activation promotes invasiveness and lymph node metastases in papillary thyroid carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDingyuan Luo,Ying Liu,Shaojian Lin,Langping Tan,Zehu Du,Miaoyun Long,Yue Zhu,Xinzhi Peng,Weixing Zong,Gerardo G Mackenzie,Honghao Li,Nengtai OuyangPubMed 30535496
- Molecular reproduction and development 85:7-162017Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria-related proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian An,Yue-Fen Wang,Jia-Xian Liu,Yan-Yun Pan,Yu-Fei Liu,Zhong-Chen He,Fang-Fang Mo,Jun Li,Li-Hua Kang,Yu-Jie Gu,Bo-Han Lv,Si-Huan Gao,Guang-Jian JiangPubMed 29149484
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SERPINB3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29508
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Associated Products
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2 products for Alternative Product
Target data
Function
May act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1).
Alternative names
SCCA, SCCA1, SERPINB3, Serpin B3, Protein T4-A, Squamous cell carcinoma antigen 1, SCCA-1
Recommended products
Rabbit Polyclonal SerpinB3/SCCA antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERPINB3 aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SERPINB3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29508
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SerpinB3 also known as Squamous Cell Carcinoma Antigen (SCCA) is part of the serine protease inhibitor family. This protein has a molecular mass of approximately 45 kDa. It is expressed in various human tissues including skin liver and the respiratory epithelium. SerpinB3 acts by inhibiting cysteine proteinases through binding to them which helps regulate their activity and prevent excessive proteolysis. Such regulatory mechanisms are essential for maintaining cellular homeostasis.
- Biological function summary
SerpinB3 plays a role in cellular proliferation differentiation and protection against apoptosis. It is not reported as part of a specific protein complex but it engages in interactions that influence cell signaling pathways. By protecting cells from programmed cell death SerpinB3 can contribute to tissue repair and regeneration. Its expression levels can change significantly during cell stress responses suggesting its involvement in adaptive processes to environmental stimuli.
- Pathways
Protein SerpinB3 is involved in inflammatory and immune pathways. It influences the NF-κB signaling pathway which is important for immune response regulation and inflammation. This pathway involves multiple proteins including IκB which acts as an inhibitor and other cytokines and transcription factors that facilitate signal transduction in response to stress. SerpinB3’s modulation of these pathways reflects how it affects both innate and adaptive immune responses.
- Associated diseases and disorders
SerpinB3 has been associated with the progression of squamous cell carcinoma and liver fibrosis. In squamous cell carcinoma its interaction with proteases like cathepsin contributes to cancer cell survival and metastasis. For liver fibrosis SerpinB3’s inhibition of apoptosis in hepatocytes may lead to excessive tissue scarring. These connections illustrate its potential as a biomarker for diagnosis and as a target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-SerpinB3/SCCA antibody (ab154971)
All lanes: Western blot - Anti-SerpinB3/SCCA antibody (ab154971) at 1/1000 dilution
Lane 1: Non-transfected 293T whole cell extracts at 30 µg
Lane 2: Transfected 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody (ab154971)
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling SerpinB3/SCCA with ab154971 at 1/500 dilution.
Western blot - Anti-SerpinB3/SCCA antibody (ab154971)
All lanes: Western blot - Anti-SerpinB3/SCCA antibody (ab154971) at 1/3000 dilution
Lane 1: A549 cell lysate at 30 µg
Lane 2: H1299 cell lysate at 30 µg
Lane 3: HCT116 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 45 kDa
Western blot - Anti-SerpinB3/SCCA antibody (ab154971)
10% SDS PAGE
All lanes: Western blot - Anti-SerpinB3/SCCA antibody (ab154971) at 1/3000 dilution
All lanes: HCT116 whole cell lysate at 30 µg
Predicted band size: 35 kDa, 45 kDa, 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody (ab154971)
Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling SerpinB3/SCCA with ab154971 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-SerpinB3/SCCA antibody (ab154971)
Immunofluorescent analysis of methanol-fixed A549 cells labeling SerpinB3/SCCA with ab154971 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
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