AB154971

Anti-SerpinB3/SCCA antibody

Name: Anti-SerpinB3/SCCA antibody (ab154971) | Abcam
Brand: Abcam Limited
SKU: AB154971
Price: 535 USD
Availability: InStock

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(9 Publications)

Rabbit Polyclonal SerpinB3/SCCA antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERPINB3 aa 50 to C-terminus.

View Alternative Names

SCCA, SCCA1, SERPINB3, Serpin B3, Protein T4-A, Squamous cell carcinoma antigen 1, SCCA-1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-SerpinB3/SCCA antibody (AB154971)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SerpinB3/SCCA antibody (AB154971)

Immunofluorescent analysis of methanol-fixed A549 cells labeling SerpinB3/SCCA with ab154971 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody (AB154971)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody (AB154971)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling SerpinB3/SCCA with ab154971 at 1/500 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody (AB154971)

Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling SerpinB3/SCCA with ab154971 at 1/500 dilution.

Unknown

Western blot - Anti-SerpinB3/SCCA antibody (AB154971)

10% SDS PAGE

All lanes:

Western blot - Anti-SerpinB3/SCCA antibody (ab154971) at 1/3000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 35 kDa,45 kDa,50 kDa

false

Supplier Data

Western blot - Anti-SerpinB3/SCCA antibody (AB154971)

All lanes:

Western blot - Anti-SerpinB3/SCCA antibody (ab154971) at 1/3000 dilution

Lane 1:

A549 cell lysate at 30 µg

Lane 2:

H1299 cell lysate at 30 µg

Lane 3:

HCT116 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-SerpinB3/SCCA antibody (AB154971)

All lanes:

Western blot - Anti-SerpinB3/SCCA antibody (ab154971) at 1/1000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SERPINB3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P29508

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SerpinB3 also known as Squamous Cell Carcinoma Antigen (SCCA) is part of the serine protease inhibitor family. This protein has a molecular mass of approximately 45 kDa. It is expressed in various human tissues including skin liver and the respiratory epithelium. SerpinB3 acts by inhibiting cysteine proteinases through binding to them which helps regulate their activity and prevent excessive proteolysis. Such regulatory mechanisms are essential for maintaining cellular homeostasis.

Biological function summary

SerpinB3 plays a role in cellular proliferation differentiation and protection against apoptosis. It is not reported as part of a specific protein complex but it engages in interactions that influence cell signaling pathways. By protecting cells from programmed cell death SerpinB3 can contribute to tissue repair and regeneration. Its expression levels can change significantly during cell stress responses suggesting its involvement in adaptive processes to environmental stimuli.

Pathways

Protein SerpinB3 is involved in inflammatory and immune pathways. It influences the NF-κB signaling pathway which is important for immune response regulation and inflammation. This pathway involves multiple proteins including IκB which acts as an inhibitor and other cytokines and transcription factors that facilitate signal transduction in response to stress. SerpinB3’s modulation of these pathways reflects how it affects both innate and adaptive immune responses.

SerpinB3 has been associated with the progression of squamous cell carcinoma and liver fibrosis. In squamous cell carcinoma its interaction with proteases like cathepsin contributes to cancer cell survival and metastasis. For liver fibrosis SerpinB3’s inhibition of apoptosis in hepatocytes may lead to excessive tissue scarring. These connections illustrate its potential as a biomarker for diagnosis and as a target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1).

See full target information SERPINB3

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cureus 16:e72365 PubMed39583399

2024

Detection of Protein Markers From Blood Samples of Cervical Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Shahana Sharmin,Maha Jamiruddin,Mohd Raeed Jamiruddin,Abul Bashar Mir Md K Islam,Chowdhury R Ahsan,Mahmuda Yasmin

Journal of immunology research 2024:8553447 PubMed38550710

2024

SerpinB3/B4 Abates Epithelial Cell-Derived CXCL8/IL-8 Expression in Chronic Rhinosinusitis with Nasal Polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangting Bu,Ming Wang,Jing Yuan,Jing Song,Ge Luan,Jiaqi Yu,Yang Wang,Ying Li,Chengshuo Wang,Luo Zhang

Nature genetics 55:2175-2188 PubMed37985817

2023

Multiomic analysis of cervical squamous cell carcinoma identifies cellular ecosystems with biological and clinical relevance.

Applications

Unspecified application

Species

Unspecified reactive species

Junpeng Fan,Funian Lu,Tianyu Qin,Wenju Peng,Xucui Zhuang,Yinuo Li,Xin Hou,Zixuan Fang,Yunyi Yang,Ensong Guo,Bin Yang,Xi Li,Yu Fu,Xiaoyan Kang,Zimeng Wu,Lili Han,Gordon B Mills,Xiangyi Ma,Kezhen Li,Peng Wu,Ding Ma,Gang Chen,Chaoyang Sun

Cell reports 42:113434 PubMed37980563

2023

SERPINB3-MYC axis induces the basal-like/squamous subtype and enhances disease progression in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuuki Ohara,Wei Tang,Huaitian Liu,Shouhui Yang,Tiffany H Dorsey,Helen Cawley,Paloma Moreno,Raj Chari,Mary R Guest,Azadeh Azizian,Jochen Gaedcke,Michael Ghadimi,Nader Hanna,Stefan Ambs,S Perwez Hussain

Journal of applied oral science : revista FOB 29:e20210374 PubMed34878006

2021

Evaluation of squamous cell carcinoma antigen 1 expression in oral squamous cell carcinoma (tumor cells and peritumoral T-lymphocytes) and verrucous carcinoma and comparison with normal oral mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Samira Derakhshan,Arvin Poosti,Amirnader Emami Razavi,Mohammad Amin Moosavi,Nazanin Mahdavi,Fereshteh Baghaei Naieni,Kambiz Kamyab Hesari,Amirsina Rahpeima

Dermatology (Basel, Switzerland) 238:236-243 PubMed34077928

2021

3D-SeboSkin Model for Human ex vivo Studies of Hidradenitis Suppurativa/Acne Inversa.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxiao Hou,Amir M Hossini,Georgios Nikolakis,Ottfried Balthasar,Andreas Kurtz,Christos C Zouboulis

Journal of the European Academy of Dermatology and Venereology : JEADV 34:846-861 PubMed31838778

2020

Alterations in innate immunity and epithelial cell differentiation are the molecular pillars of hidradenitis suppurativa.

Applications

Unspecified application

Species

Unspecified reactive species

C C Zouboulis,A Nogueira da Costa,E Makrantonaki,X X Hou,D Almansouri,J T Dudley,H Edwards,B Readhead,O Balthasar,G B E Jemec,N G Bonitsis,G Nikolakis,D Trebing,K C Zouboulis,A M Hossini

Oncology reports 41:1253-1263 PubMed30535496

2018

Oncogenic Ras/squamous cell carcinoma antigen signaling pathway activation promotes invasiveness and lymph node metastases in papillary thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dingyuan Luo,Ying Liu,Shaojian Lin,Langping Tan,Zehu Du,Miaoyun Long,Yue Zhu,Xinzhi Peng,Weixing Zong,Gerardo G Mackenzie,Honghao Li,Nengtai Ouyang

Molecular reproduction and development 85:7-16 PubMed29149484

2017

Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria-related proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Tian An,Yue-Fen Wang,Jia-Xian Liu,Yan-Yun Pan,Yu-Fei Liu,Zhong-Chen He,Fang-Fang Mo,Jun Li,Li-Hua Kang,Yu-Jie Gu,Bo-Han Lv,Si-Huan Gao,Guang-Jian Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SerpinB3/SCCA antibody

Immunocytochemistry/ Immunofluorescence - Anti-SerpinB3/SCCA antibody (AB154971)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody (AB154971)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody (AB154971)

Western blot - Anti-SerpinB3/SCCA antibody (AB154971)

Western blot - Anti-SerpinB3/SCCA antibody (AB154971)

Western blot - Anti-SerpinB3/SCCA antibody (AB154971)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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