AB180396

Anti-SerpinB3/SCCA antibody [OTI1A5]

Name: Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396) | Abcam
Brand: Abcam Limited
SKU: AB180396

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(3 Publications)

Mouse Monoclonal SerpinB3/SCCA antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SERPINB3.

View Alternative Names

SCCA, SCCA1, SERPINB3, Serpin B3, Protein T4-A, Squamous cell carcinoma antigen 1, SCCA-1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (AB180396)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (AB180396)

Paraffin-embedded human pancreas tissue stained for SerpinB3/SCCA using ab180396 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (AB180396)

Paraffin-embedded human bladder carcinoma tissue stained for SerpinB3/SCCA using ab180396 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (AB180396)

Paraffin-embedded human kidney tissue stained for SerpinB3/SCCA using ab180396 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (AB180396)

Paraffin-embedded human lung carcinoma tissue stained for SerpinB3/SCCA using ab180396 at 1/150 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-SerpinB3/SCCA antibody [OTI1A5] (AB180396)

All lanes:

Western blot - Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY SerpinB3/SCCA cDNA at 5 µg

Predicted band size: 30 kDa,45 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1A5

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SERPINB3.

P29508

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }

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Product details

Clone OTI1A5 (formerly 1A5).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant by affinity chromatography.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SerpinB3 also known as Squamous Cell Carcinoma Antigen (SCCA) is part of the serine protease inhibitor family. This protein has a molecular mass of approximately 45 kDa. It is expressed in various human tissues including skin liver and the respiratory epithelium. SerpinB3 acts by inhibiting cysteine proteinases through binding to them which helps regulate their activity and prevent excessive proteolysis. Such regulatory mechanisms are essential for maintaining cellular homeostasis.

Biological function summary

SerpinB3 plays a role in cellular proliferation differentiation and protection against apoptosis. It is not reported as part of a specific protein complex but it engages in interactions that influence cell signaling pathways. By protecting cells from programmed cell death SerpinB3 can contribute to tissue repair and regeneration. Its expression levels can change significantly during cell stress responses suggesting its involvement in adaptive processes to environmental stimuli.

Pathways

Protein SerpinB3 is involved in inflammatory and immune pathways. It influences the NF-κB signaling pathway which is important for immune response regulation and inflammation. This pathway involves multiple proteins including IκB which acts as an inhibitor and other cytokines and transcription factors that facilitate signal transduction in response to stress. SerpinB3’s modulation of these pathways reflects how it affects both innate and adaptive immune responses.

SerpinB3 has been associated with the progression of squamous cell carcinoma and liver fibrosis. In squamous cell carcinoma its interaction with proteases like cathepsin contributes to cancer cell survival and metastasis. For liver fibrosis SerpinB3’s inhibition of apoptosis in hepatocytes may lead to excessive tissue scarring. These connections illustrate its potential as a biomarker for diagnosis and as a target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1).

See full target information SERPINB3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1240 PubMed39890781

2025

Proteomic profiling identifies muscle-invasive bladder cancers with distinct biology and responses to platinum-based chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

A Contreras-Sanz,G L Negri,M J Reike,H Z Oo,J M Scurll,S E Spencer,K Nielsen,K Ikeda,G Wang,C L Jackson,S Gupta,M E Roberts,D M Berman,R Seiler,G B Morin,P C Black

Genes 14: PubMed36833193

2023

, Adult-Onset Immunodeficiency, and Generalized Pustular Psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Piranit Kantaputra,Teerada Daroontum,Mati Chuamanochan,Suteeraporn Chaowattanapanit,Salin Kiratikanon,Charoen Choonhakarn,Worrachet Intachai,Bjorn Olsen,Sissades Tongsima,Chumpol Ngamphiw,Patrizia Pontisso,Timothy C Cox,Puey Ounjai

Thoracic cancer 12:2666-2679 PubMed34453496

2021

Comprehensive expressional analysis of chemosensitivity-related markers in large cell neuroendocrine carcinoma of the lung.

Applications

Unspecified application

Species

Unspecified reactive species

Yoichi Ohtaki,Kyoichi Kaira,Toshiki Yajima,Bilguun Erkhem-Ochir,Osamu Kawashima,Mitsuhiro Kamiyoshihara,Hitoshi Igai,Ryoichi Onozato,Takashi Ibe,Takayuki Kosaka,Seshiru Nakazawa,Toshiteru Nagashima,Tetsunari Oyama,Ken Shirabe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SerpinB3/SCCA antibody [OTI1A5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (AB180396)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (AB180396)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (AB180396)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (AB180396)

Western blot - Anti-SerpinB3/SCCA antibody [OTI1A5] (AB180396)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise