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AB154591

Anti-SERPINE2/PN-1 antibody

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(4 Publications)

Rabbit Polyclonal SERPINE2/PN-1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERPINE2 aa 150 to C-terminus.

View Alternative Names

PI7, PN1, SERPINE2, Glia-derived nexin, GDN, Peptidase inhibitor 7, Protease nexin 1, Protease nexin I, Serpin E2, PI-7, PN-1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-SERPINE2/PN-1 antibody (AB154591)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SERPINE2/PN-1 antibody (AB154591)

Immunofluorescence analysis of methanol-fixed HeLa cells labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINE2/PN-1 antibody (AB154591)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINE2/PN-1 antibody (AB154591)

Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution.

Western blot - Anti-SERPINE2/PN-1 antibody (AB154591)
  • WB

Unknown

Western blot - Anti-SERPINE2/PN-1 antibody (AB154591)

10% SDS PAGE

All lanes:

Western blot - Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 44 kDa

false

Western blot - Anti-SERPINE2/PN-1 antibody (AB154591)
  • WB

Unknown

Western blot - Anti-SERPINE2/PN-1 antibody (AB154591)

10% SDS PAGE

All lanes:

Western blot - Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SERPINE2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P07093

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Have you thought about this alternative?

AB134905

Anti-SERPINE2/PN-1 antibody [EPR8776]

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SERPINE2/PN-1 also known as protease nexin-1 (PN1) is a serine protease inhibitor with a molecular mass of approximately 47 kDa. This inhibitory protein predominantly expresses in various tissues including the brain lungs and liver. In the central nervous system it is abundantly present and plays important roles in glial cell function. It acts by binding and inhibiting multiple serine proteases such as thrombin plasmin and trypsin. PN-1 exerts significant mechanical control over these proteases regulating processes like coagulation and fibrinolysis.
Biological function summary

The protein PN-1 is important for maintaining tissue homeostasis and controlling extracellular matrix remodeling. PN-1 does not form part of a larger complex but interacts directly with its target proteases. It shields tissues from excessive proteolytic activity therefore preventing unwarranted degradation of proteins. The protein's inhibitory action is non-specific allowing it to modulate various biological processes from inflammatory responses to tissue recovery through its action across different proteases.

Pathways

PN-1 plays a role in the regulation of coagulation and fibrinolytic pathways. In the coagulation pathway its interaction with thrombin is significant inhibiting thrombin’s activity and therefore controlling blood clot formation. In the fibrinolytic pathway PN-1 modulates the activity of plasmin an essential protease for degrading fibrin clots. By regulating these pathways PN-1 interacts with proteins such as fibrinogen and other regulatory elements within these biological systems.

The function of PN-1 connects closely with cardiovascular diseases and neurodegenerative disorders. In cardiovascular diseases excessive or insufficient activity of PN-1 may contribute to improper blood clot formation linking this protein to conditions like thrombosis. In the context of neurodegenerative disorders altered levels of PN-1 influence the activity of neurological proteases potentially impacting diseases like Alzheimer’s. PN-1 may interact with proteins like α-2-macroglobulin in these disease processes emphasizing its role in pathophysiological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.
See full target information SERPINE2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:108370 PubMed38034348

2023

Molecular subtyping and characterization of clear cell renal cell carcinoma by tumor differentiation trajectories.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu-Wu Pan,Wen-Jin Chen,Da Xu,Wen-Bin Guan,Lin Li,Jia-Xin Chen,Wei-Jie Chen,Ke-Qin Dong,Jian-Qing Ye,Si-Shun Gan,Wang Zhou,Xin-Gang Cui

Stem cell research & therapy 14:14 PubMed36721241

2023

Combining single-cell transcriptomics and CellTagging to identify differentiation trajectories of human adipose-derived mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Lin,Yanlei Yang,Yinghao Cao,Junbo Liang,Jun Qian,Xiaoyue Wang,Qin Han

Molecular medicine reports 16:2417-2424 PubMed28677735

2017

miR‑199a‑3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Bo Li,Qun Wu,Jie Liu,Yong-Zhi Fan,Kai-Feng Yu,Yi Cai

Scientific reports 6:30563 PubMed27460424

2016

The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xinghuo Wu,Wei Liu,Zhenfeng Duan,Yong Gao,Shuai Li,Kun Wang,Yu Song,Zengwu Shao,Shuhua Yang,Cao Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
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Anti-SERPINE2/PN-1 antibody

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(0 reviews)

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