Rabbit Polyclonal SERPINE2/PN-1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Glia-derived nexin aa 150 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.
PI7, PN1, PN1, PI7, SERPINE2, Glia-derived nexin, GDN, Peptidase inhibitor 7, Protease nexin 1, Protease nexin I, Serpin E2, PI-7, PN-1
Rabbit Polyclonal SERPINE2/PN-1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Glia-derived nexin aa 150 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
SERPINE2/PN-1 also known as protease nexin-1 (PN1) is a serine protease inhibitor with a molecular mass of approximately 47 kDa. This inhibitory protein predominantly expresses in various tissues including the brain lungs and liver. In the central nervous system it is abundantly present and plays important roles in glial cell function. It acts by binding and inhibiting multiple serine proteases such as thrombin plasmin and trypsin. PN-1 exerts significant mechanical control over these proteases regulating processes like coagulation and fibrinolysis.
The protein PN-1 is important for maintaining tissue homeostasis and controlling extracellular matrix remodeling. PN-1 does not form part of a larger complex but interacts directly with its target proteases. It shields tissues from excessive proteolytic activity therefore preventing unwarranted degradation of proteins. The protein's inhibitory action is non-specific allowing it to modulate various biological processes from inflammatory responses to tissue recovery through its action across different proteases.
PN-1 plays a role in the regulation of coagulation and fibrinolytic pathways. In the coagulation pathway its interaction with thrombin is significant inhibiting thrombin’s activity and therefore controlling blood clot formation. In the fibrinolytic pathway PN-1 modulates the activity of plasmin an essential protease for degrading fibrin clots. By regulating these pathways PN-1 interacts with proteins such as fibrinogen and other regulatory elements within these biological systems.
The function of PN-1 connects closely with cardiovascular diseases and neurodegenerative disorders. In cardiovascular diseases excessive or insufficient activity of PN-1 may contribute to improper blood clot formation linking this protein to conditions like thrombosis. In the context of neurodegenerative disorders altered levels of PN-1 influence the activity of neurological proteases potentially impacting diseases like Alzheimer’s. PN-1 may interact with proteins like α-2-macroglobulin in these disease processes emphasizing its role in pathophysiological conditions.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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10% SDS PAGE
All lanes: Western blot - Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution
Lane 1: HeLa whole cell lysate at 30 µg
Lane 2: HepG2 whole cell lysate at 30 µg
Predicted band size: 44 kDa
10% SDS PAGE
All lanes: Western blot - Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 44 kDa
Immunofluorescence analysis of methanol-fixed HeLa cells labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution.
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