AB134905

Anti-SERPINE2/PN-1 antibody [EPR8776]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal SERPINE2/PN-1 antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 4 publications.

View Alternative Names

PI7, PN1, SERPINE2, Glia-derived nexin, GDN, Peptidase inhibitor 7, Protease nexin 1, Protease nexin I, Serpin E2, PI-7, PN-1

2 Images

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Western blot - Anti-SERPINE2/PN-1 antibody [EPR8776] (AB134905)

All lanes:

Western blot - Anti-SERPINE2/PN-1 antibody [EPR8776] (ab134905) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

Human oligodendroglioma cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 44 kDa

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OI-RD Scanning - Anti-SERPINE2/PN-1 antibody [EPR8776] (AB134905)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SERPINE2/PN-1 antibody [EPR8776] (AB134905)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-SERPINE2/PN-1 antibody [EPR8776] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8776

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SERPINE2/PN-1 also known as protease nexin-1 (PN1) is a serine protease inhibitor with a molecular mass of approximately 47 kDa. This inhibitory protein predominantly expresses in various tissues including the brain lungs and liver. In the central nervous system it is abundantly present and plays important roles in glial cell function. It acts by binding and inhibiting multiple serine proteases such as thrombin plasmin and trypsin. PN-1 exerts significant mechanical control over these proteases regulating processes like coagulation and fibrinolysis.

Biological function summary

The protein PN-1 is important for maintaining tissue homeostasis and controlling extracellular matrix remodeling. PN-1 does not form part of a larger complex but interacts directly with its target proteases. It shields tissues from excessive proteolytic activity therefore preventing unwarranted degradation of proteins. The protein's inhibitory action is non-specific allowing it to modulate various biological processes from inflammatory responses to tissue recovery through its action across different proteases.

Pathways

PN-1 plays a role in the regulation of coagulation and fibrinolytic pathways. In the coagulation pathway its interaction with thrombin is significant inhibiting thrombin’s activity and therefore controlling blood clot formation. In the fibrinolytic pathway PN-1 modulates the activity of plasmin an essential protease for degrading fibrin clots. By regulating these pathways PN-1 interacts with proteins such as fibrinogen and other regulatory elements within these biological systems.

The function of PN-1 connects closely with cardiovascular diseases and neurodegenerative disorders. In cardiovascular diseases excessive or insufficient activity of PN-1 may contribute to improper blood clot formation linking this protein to conditions like thrombosis. In the context of neurodegenerative disorders altered levels of PN-1 influence the activity of neurological proteases potentially impacting diseases like Alzheimer’s. PN-1 may interact with proteins like α-2-macroglobulin in these disease processes emphasizing its role in pathophysiological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.

See full target information SERPINE2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Canadian journal of gastroenterology & hepatology 2025:5209381 PubMed41031049

2025

TSPAN7 Functions as an Antitumor Agent Through the STK11/AMPK/mTOR Axis in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Weng,Fenfen Hong

Cell death & disease 14:30 PubMed36646679

2023

Single-cell RNA-seq integrated with multi-omics reveals SERPINE2 as a target for metastasis in advanced renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Jin Chen,Ke-Qin Dong,Xiu-Wu Pan,Si-Shun Gan,Da Xu,Jia-Xin Chen,Wei-Jie Chen,Wen-Yan Li,Yu-Qi Wang,Wang Zhou,Brian Rini,Xin-Gang Cui

International journal of molecular sciences 23: PubMed36142828

2022

A Novel L-Phenylalanine Dipeptide Inhibits the Growth and Metastasis of Prostate Cancer Cells via Targeting DUSP1 and TNFSF9.

Applications

Unspecified application

Species

Unspecified reactive species

Lanlan Li,Mingfei Yang,Jia Yu,Sha Cheng,Mashaal Ahmad,Caihong Wu,Xinwei Wan,Bixue Xu,Yaacov Ben-David,Heng Luo

Cancer cell 35:868-884.e6 PubMed31185211

2019

Symbiotic Macrophage-Glioma Cell Interactions Reveal Synthetic Lethality in PTEN-Null Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Peiwen Chen,Di Zhao,Jun Li,Xin Liang,Jiexi Li,Andrew Chang,Verlene K Henry,Zhengdao Lan,Denise J Spring,Ganesh Rao,Y Alan Wang,Ronald A DePinho

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SERPINE2/PN-1 antibody [EPR8776]

Western blot - Anti-SERPINE2/PN-1 antibody [EPR8776] (AB134905)

OI-RD Scanning - Anti-SERPINE2/PN-1 antibody [EPR8776] (AB134905)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise