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AB248719

Anti-SERPINE2/PN-1 antibody [EPR8776] - BSA and Azide free

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Rabbit Recombinant Monoclonal SERPINE2/PN-1 antibody. Carrier free. Suitable for WB and reacts with Rat, Human samples.

View Alternative Names

PI7, PN1, SERPINE2, Glia-derived nexin, GDN, Peptidase inhibitor 7, Protease nexin 1, Protease nexin I, Serpin E2, PI-7, PN-1

2 Images
Western blot - Anti-SERPINE2/PN-1 antibody [EPR8776] - BSA and Azide free (AB248719)
  • WB

Unknown

Western blot - Anti-SERPINE2/PN-1 antibody [EPR8776] - BSA and Azide free (AB248719)

This data was developed using ab134905, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SERPINE2/PN-1 antibody [EPR8776] (<a href='/en-us/products/primary-antibodies/serpine2-pn-1-antibody-epr8776-ab134905'>ab134905</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

Human oligodendroglioma cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 44 kDa

false

OI-RD Scanning - Anti-SERPINE2/PN-1 antibody [EPR8776] - BSA and Azide free (AB248719)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SERPINE2/PN-1 antibody [EPR8776] - BSA and Azide free (AB248719)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8776

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab248719 is the carrier-free version of ab134905.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SERPINE2/PN-1 also known as protease nexin-1 (PN1) is a serine protease inhibitor with a molecular mass of approximately 47 kDa. This inhibitory protein predominantly expresses in various tissues including the brain lungs and liver. In the central nervous system it is abundantly present and plays important roles in glial cell function. It acts by binding and inhibiting multiple serine proteases such as thrombin plasmin and trypsin. PN-1 exerts significant mechanical control over these proteases regulating processes like coagulation and fibrinolysis.
Biological function summary

The protein PN-1 is important for maintaining tissue homeostasis and controlling extracellular matrix remodeling. PN-1 does not form part of a larger complex but interacts directly with its target proteases. It shields tissues from excessive proteolytic activity therefore preventing unwarranted degradation of proteins. The protein's inhibitory action is non-specific allowing it to modulate various biological processes from inflammatory responses to tissue recovery through its action across different proteases.

Pathways

PN-1 plays a role in the regulation of coagulation and fibrinolytic pathways. In the coagulation pathway its interaction with thrombin is significant inhibiting thrombin’s activity and therefore controlling blood clot formation. In the fibrinolytic pathway PN-1 modulates the activity of plasmin an essential protease for degrading fibrin clots. By regulating these pathways PN-1 interacts with proteins such as fibrinogen and other regulatory elements within these biological systems.

The function of PN-1 connects closely with cardiovascular diseases and neurodegenerative disorders. In cardiovascular diseases excessive or insufficient activity of PN-1 may contribute to improper blood clot formation linking this protein to conditions like thrombosis. In the context of neurodegenerative disorders altered levels of PN-1 influence the activity of neurological proteases potentially impacting diseases like Alzheimer’s. PN-1 may interact with proteins like α-2-macroglobulin in these disease processes emphasizing its role in pathophysiological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.
See full target information SERPINE2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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