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AB180774

Anti-SERPING1 antibody

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(1 Publication)

Rabbit Polyclonal SERPING1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SERPING1 aa 200 to C-terminus.

View Alternative Names

C1IN, C1NH, SERPING1, Plasma protease C1 inhibitor, C1 Inh, C1Inh, C1 esterase inhibitor, C1-inhibiting factor, Serpin G1

1 Images
Western blot - Anti-SERPING1 antibody (AB180774)
  • WB

Supplier Data

Western blot - Anti-SERPING1 antibody (AB180774)

Blocking and diluting buffer and concentration : 3% nonfat dry milk in TBST.

ab180774 detects a highly glycosylated plasma protein hence the difference in MW from predicted size.

All lanes:

Western blot - Anti-SERPING1 antibody (ab180774) at 1/1000 dilution

All lanes:

Human plasma at 25 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 55 kDa

Observed band size: 100 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human SERPING1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05155

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SERPING1 also known as C1 inhibitor or C1-inactivator is a protein that plays an important mechanical role in the regulation of the complement system. It is a member of the serpin superfamily and has a molecular mass of approximately 105-110 kDa. This protein is mainly expressed in the liver but also present in other tissues such as monocytes and epithelial cells. As an inhibitor SERPING1 effectively regulates the activity of the complement subcomponents C1r and C1s plasmin and kallikrein by inhibiting their protease activity preventing unwanted activation of the complement cascade.
Biological function summary

The protein acts as a critical control point in the complement and contact activation system. It is part of the C1 complex which includes the proteins C1q C1r and C1s. The regulation extends to pathways that involve the breakdown of plasma proteins and prevention of spontaneous inflammatory reactions. By controlling these pathways the protein prevents excessive inflammation and tissue damage which can occur during immune responses. Its involvement extends to the regulation of the complement system and maintenance of vascular permeability.

Pathways

SERPING1 interacts closely with mechanisms involved in the complement cascade and the kinin-kallikrein pathways. It associates with proteins like MASP-1 and MASP-2 important in the complement and lectin pathways. These pathways are important for innate immunity and influence factors like inflammation and blood pressure. SERPING1 modulates these pathways by inhibiting key enzymes and ensuring controlled responses therefore maintaining systemic balance within the immune system.

The malfunction or deficiency of SERPING1 has direct links to hereditary angioedema a condition marked by severe and rapid swelling episodes. The disorder results from inadequate inhibition of bradykinin emphasizing the protein's role in vascular regulation. Additionally the protein has implications in autoimmune diseases such as systemic lupus erythematosus where complement regulation is disrupted. The interplay between SERPING1 and other proteins such as bradykinin receptor B2 remains significant in understanding disease mechanisms and developing therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.
See full target information SERPING1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology research 7:230-243 PubMed30563830

2018

A Mechanism of Resistance to Antibody-Targeted Immune Attack.

Applications

Unspecified application

Species

Unspecified reactive species

Dalal S Aldeghaither,David J Zahavi,Joseph C Murray,Elana J Fertig,Garrett T Graham,Yong-Wei Zhang,Allison O'Connell,Junfeng Ma,Sandra A Jablonski,Louis M Weiner
View all publications
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