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AB134915

Anti-SERPING1 antibody [EPR8016]

5

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal SERPING1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

C1IN, C1NH, SERPING1, Plasma protease C1 inhibitor, C1 Inh, C1Inh, C1 esterase inhibitor, C1-inhibiting factor, Serpin G1

2 Images
Western blot - Anti-SERPING1 antibody [EPR8016] (AB134915)
  • WB

Unknown

Western blot - Anti-SERPING1 antibody [EPR8016] (AB134915)

All lanes:

Western blot - Anti-SERPING1 antibody [EPR8016] (ab134915) at 1/1000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human serum lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 55 kDa

false

OI-RD Scanning - Anti-SERPING1 antibody [EPR8016] (AB134915)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SERPING1 antibody [EPR8016] (AB134915)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-SERPING1 antibody [EPR8016] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8016

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SERPING1 also known as C1 inhibitor or C1-inactivator is a protein that plays an important mechanical role in the regulation of the complement system. It is a member of the serpin superfamily and has a molecular mass of approximately 105-110 kDa. This protein is mainly expressed in the liver but also present in other tissues such as monocytes and epithelial cells. As an inhibitor SERPING1 effectively regulates the activity of the complement subcomponents C1r and C1s plasmin and kallikrein by inhibiting their protease activity preventing unwanted activation of the complement cascade.
Biological function summary

The protein acts as a critical control point in the complement and contact activation system. It is part of the C1 complex which includes the proteins C1q C1r and C1s. The regulation extends to pathways that involve the breakdown of plasma proteins and prevention of spontaneous inflammatory reactions. By controlling these pathways the protein prevents excessive inflammation and tissue damage which can occur during immune responses. Its involvement extends to the regulation of the complement system and maintenance of vascular permeability.

Pathways

SERPING1 interacts closely with mechanisms involved in the complement cascade and the kinin-kallikrein pathways. It associates with proteins like MASP-1 and MASP-2 important in the complement and lectin pathways. These pathways are important for innate immunity and influence factors like inflammation and blood pressure. SERPING1 modulates these pathways by inhibiting key enzymes and ensuring controlled responses therefore maintaining systemic balance within the immune system.

The malfunction or deficiency of SERPING1 has direct links to hereditary angioedema a condition marked by severe and rapid swelling episodes. The disorder results from inadequate inhibition of bradykinin emphasizing the protein's role in vascular regulation. Additionally the protein has implications in autoimmune diseases such as systemic lupus erythematosus where complement regulation is disrupted. The interplay between SERPING1 and other proteins such as bradykinin receptor B2 remains significant in understanding disease mechanisms and developing therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.
See full target information SERPING1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 3:441-459 PubMed38765203

2024

Histone H1.0 couples cellular mechanical behaviors to chromatin structure.

Applications

Unspecified application

Species

Unspecified reactive species

Shuaishuai Hu,Douglas J Chapski,Natalie D Gehred,Todd H Kimball,Tatiana Gromova,Angelina Flores,Amy C Rowat,Junjie Chen,René R Sevag Packard,Emily Olszewski,Jennifer Davis,Christoph D Rau,Timothy A McKinsey,Manuel Rosa-Garrido,Thomas M Vondriska

PloS one 18:e0287829 PubMed37910521

2023

Interleukin-27-induced HIV-resistant dendritic cells suppress reveres transcription following virus entry in an SPTBN1, autophagy, and YB-1 independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Tomozumi Imamichi,Qian Chen,Bharatwaj Sowrirajan,Jun Yang,Sylvain Laverdure,Mayra Marquez,Anthony R Mele,Catherine Watkins,Joseph W Adelsberger,Jeanette Higgins,Hongyan Sui
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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