JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB235995

Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal Serum Amyloid A 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Serum amyloid A-1 protein, SAA, SAA1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free (AB235995)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free (AB235995)

This data was developed using ab190802 the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue sections labeling Serum Amyloid A with Purified ab190802 at 1 : 400 dilution (0.96 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Western blot - Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free (AB235995)
  • WB

Unknown

Western blot - Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free (AB235995)

This data was developed using ab190802, the same antibody clone in a different buffer formulation.

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Serum Amyloid A antibody [EP11592-92] (<a href='/en-us/products/primary-antibodies/serum-amyloid-a-antibody-ep11592-92-ab190802'>ab190802</a>) at 1/1000 dilution

All lanes:

Human plasma lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 14 kDa

Observed band size: 12 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP11592-92

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab235995 is the carrier-free version of ab190802.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid A (SAA) is an important acute-phase protein with a mass of approximately 12 kDa. It plays a role in inflammation and is present in the serum. SAA is an apolipoprotein that associates with high-density lipoprotein (HDL) during inflammatory states. The protein is expressed mainly in the liver and circulating SAA levels greatly increase during inflammatory responses marking it as an important marker for inflammation.
Biological function summary

SAA contributes to the body's immune response to injury by recruiting immune cells and facilitating the removal of cholesterol. As part of the acute-phase response SAA integrates into HDL displacing other apolipoproteins and altering HDL's function. Increased SAA levels can also modulate prostaglandin synthesis and secretion influencing the inflammatory response. The exact role of SAA in pathways beyond these functions continues to be a subject of research.

Pathways

SAA influences lipid metabolism and inflammatory pathways. It plays a significant role within the acute-phase response pathway which modulates the immune system’s reaction to stress such as infection or injury. Another pathway involves cholesterol metabolism where SAA interacts with apolipoproteins AI and AII altering the composition and function of HDL particles.

High levels of SAA associate with amyloidosis and chronic inflammatory diseases. In amyloidosis misfolded SAA proteins deposit as amyloid fibrils in tissues leading to organ dysfunction. In dogs and other species SAA is used as a biomarker to monitor inflammatory diseases. Its connection with C-reactive protein further emphasizes its role in inflammatory processes. Research continues to explore how modulating SAA levels may impact disease progression and treatment.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Major acute phase protein.
See full target information SAA1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging neuroscience 12:575481 PubMed33328957

2020

Alpha-Synuclein Accumulation and Its Phosphorylation in the Enteric Nervous System of Patients Without Neurodegeneration: An Explorative Study.

Applications

Unspecified application

Species

Unspecified reactive species

Lu-Lu Bu,Kai-Xun Huang,De-Zhi Zheng,Dan-Yu Lin,Ying Chen,Xiu-Na Jing,Yan-Ran Liang,En-Xiang Tao
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB687

Anti-Serum Amyloid A antibody [115]

Western blot - Anti-Serum Amyloid A antibody [115] (AB687)

  • 4
  • 6
Primary Antibodies

AB190801

Anti-Serum Amyloid A antibody [EPR11592-129]

RabMAb|Recombinant

Western blot - Anti-Serum Amyloid A antibody [EPR11592-129] (AB190801)

  • 0
  • 0

Complementary Products

Assay Kits

AB100635

Human SAA ELISA Kit (SAA1)

Sandwich ELISA - Human SAA ELISA Kit (SAA1) (AB100635)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32412

Anti-C Reactive Protein antibody [Y284]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412)

  • 5
  • 6
Primary Antibodies

AB133575

Anti-IL-10 antibody [EPR1114]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (AB133575)

  • 4
  • 4
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com