AB251043

Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free

RabMAb
Recombinant
What is this?

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Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (ab251043) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Serum Amyloid A in Western Blot. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Serum amyloid A-1 protein, SAA, SAA1

2 Images

Supplier Data

Western blot - Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (AB251043)

This data was developed using ab190801, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Serum Amyloid A antibody [EPR11592-129] (<a href='/en-us/products/primary-antibodies/serum-amyloid-a-antibody-epr11592-129-ab190801'>ab190801</a>) at 1/1000 dilution

Lane 1:

Human serum lysate at 10 µg

Lane 2:

Human plasma lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 12 kDa

false

Supplier Data

Western blot - Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (AB251043)

This data was developed using ab190801, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Serum Amyloid A antibody [EPR11592-129] (<a href='/en-us/products/primary-antibodies/serum-amyloid-a-antibody-epr11592-129-ab190801'>ab190801</a>) at 1/5000 dilution

All lanes:

Human Serum Amyloid A2 recombinant protein (UniProt P0DJI9) with proprietary tag at 0.01 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11592-129

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (ab251043) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of Serum Amyloid A?
Anti-Serum Amyloid A [EPR11592-129] - BSA and Azide free (ab251043) specifically detects a band for Serum Amyloid A (UniProt: P0DJI8) at a molecular weight of 14kDa.

Other related products
We have a range of other formats of antibody clone [EPR11592-129] also available for your convenience: ab190801, Carrier free - ab251043

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid A (SAA) is an important acute-phase protein with a mass of approximately 12 kDa. It plays a role in inflammation and is present in the serum. SAA is an apolipoprotein that associates with high-density lipoprotein (HDL) during inflammatory states. The protein is expressed mainly in the liver and circulating SAA levels greatly increase during inflammatory responses marking it as an important marker for inflammation.

Biological function summary

SAA contributes to the body's immune response to injury by recruiting immune cells and facilitating the removal of cholesterol. As part of the acute-phase response SAA integrates into HDL displacing other apolipoproteins and altering HDL's function. Increased SAA levels can also modulate prostaglandin synthesis and secretion influencing the inflammatory response. The exact role of SAA in pathways beyond these functions continues to be a subject of research.

Pathways

SAA influences lipid metabolism and inflammatory pathways. It plays a significant role within the acute-phase response pathway which modulates the immune system’s reaction to stress such as infection or injury. Another pathway involves cholesterol metabolism where SAA interacts with apolipoproteins AI and AII altering the composition and function of HDL particles.

High levels of SAA associate with amyloidosis and chronic inflammatory diseases. In amyloidosis misfolded SAA proteins deposit as amyloid fibrils in tissues leading to organ dysfunction. In dogs and other species SAA is used as a biomarker to monitor inflammatory diseases. Its connection with C-reactive protein further emphasizes its role in inflammatory processes. Research continues to explore how modulating SAA levels may impact disease progression and treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major acute phase protein.

See full target information SAA1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free

Western blot - Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (AB251043)

Western blot - Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (AB251043)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise