AB45151

Anti-Serum Amyloid P/SAP antibody [EP1018Y]

Name: Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) | Abcam
Brand: Abcam Limited
SKU: AB45151
Price: 5005 USD
Availability: InStock
Rating: 5 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(11 Publications)

Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) is a rabbit monoclonal antibody detecting Serum Amyloid P/SAP in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
- Trusted since 2006

View Alternative Names

PTX2, APCS, Serum amyloid P-component, SAP, 9.5S alpha-1-glycoprotein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB45151)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB45151)

ab45151 staining Serum Amyloid P/SAP in human endometrial carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1 : PBS in place of primary antibody.

Lab

Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB45151)

Blocking and diluting buffer : 5% NFDM /TBST

All lanes:

Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) at 1/5000 dilution

All lanes:

Human liver at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 25 kDa

false

Lab

Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB45151)

Diluting and blocking buffer : 5% NFDM /TBST

All lanes:

Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) at 1/10000 dilution

All lanes:

Human serum at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 25 kDa

false

Carrier free

Anti-Serum Amyloid P/SAP antibody [EP1018Y] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Serum Amyloid P/SAP antibody [EP1018Y]
HRP

HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1018Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/250", "IHCP-species-notes": "<p>Enzymatic antigen retrieval (using protease) also has been found to work and may acheive better results</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Serum Amyloid P/SAP?
Anti-Serum Amyloid P/SAP [EP1018Y] (ab45151) specifically detects a band for Serum Amyloid P/SAP (UniProt: P02743) at a molecular weight of 25kDa.

Trusted by the scientific community
Anti-Serum Amyloid P/SAP [EP1018Y] (ab45151) was first used in a scientific publication in 2006 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EP1018Y] also available for your convenience: ab45151, Carrier free - ab232468, Alexa Fluor® 488 - ab322324, HRP - ab323994

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid P component also known as SAP P-SAP or APCS protein is a pentraxin family member. It has a molecular mass of approximately 25 kDa. SAP expresses mainly in the liver and circulates in blood plasma. The protein consists of five identical subunits which form a homopentameric structure. This structure enables SAP to bind to amyloid fibrils a defining mechanical action of this protein.

Biological function summary

SAP plays a role in the immune system by recognizing damaged cells and pathogens. It acts as an opsonin marking these cellular targets for phagocytosis. As part of a complex SAP stabilizes extracellular matrix and amyloid deposits. It binds specifically to amyloid fibrils found in various tissues potentially facilitating their removal by immune cells. The protein's binding actions are important for its role in maintaining tissue and fluid homeostasis.

Pathways

SAP is actively involved in the innate immune response and amyloidogenic pathways. It interacts with the complement system to enhance the clearance of cell debris and amyloid fibrils. The complement system is an important component of innate immunity where SAP can engage with complement proteins like C1q. This interaction suggests that SAP plays roles in modulating both inflammation and tissue repair processes.

SAP has significant connections. SAP is related to systemic amyloidosis where it binds to amyloid deposits affecting multiple organs. Moreover SAP has been implicated in Alzheimer's disease due to its interaction with beta-amyloid protein plaques. In these disorders the involvement of SAP aids researchers in understanding the pathological mechanisms and could open avenues for targeted therapies aimed at modulating SAP functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.

See full target information APCS

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Clinical proteomics 17:20 PubMed32518534

2020

Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hyun Lee,Jae Hun Jung,Jeesoo Kim,Won-Ki Baek,Jinseol Rhee,Tae-Hwan Kim,Sang-Hyon Kim,Kwang Pyo Kim,Chang-Nam Son,Jong-Seo Kim

PloS one 15:e0227227 PubMed31978114

2020

Serum amyloid P component promotes formation of distinct aggregated lysozyme morphologies and reduces toxicity in Drosophila flies expressing F57I lysozyme.

Applications

Unspecified application

Species

Unspecified reactive species

Liza Bergkvist,Daniel R Richards,Ana Bernardo-Gancedo,Janet R Kumita,Peter R Nilsson,Ann-Christin Brorsson

Neuron 105:975-991.e7 PubMed31926610

2020

Deep Multilayer Brain Proteomics Identifies Molecular Networks in Alzheimer's Disease Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Bai,Xusheng Wang,Yuxin Li,Ping-Chung Chen,Kaiwen Yu,Kaushik Kumar Dey,Jay M Yarbro,Xian Han,Brianna M Lutz,Shuquan Rao,Yun Jiao,Jeffrey M Sifford,Jonghee Han,Minghui Wang,Haiyan Tan,Timothy I Shaw,Ji-Hoon Cho,Suiping Zhou,Hong Wang,Mingming Niu,Ariana Mancieri,Kaitlynn A Messler,Xiaojun Sun,Zhiping Wu,Vishwajeeth Pagala,Anthony A High,Wenjian Bi,Hui Zhang,Hongbo Chi,Vahram Haroutunian,Bin Zhang,Thomas G Beach,Gang Yu,Junmin Peng

Infection and immunity 86: PubMed29531132

2018

Serum Amyloid P and IgG Exhibit Differential Capabilities in the Activation of the Innate Immune System in Response to Bacillus anthracis Peptidoglycan.

Applications

Unspecified application

Species

Unspecified reactive species

Alanson W Girton,Narcis I Popescu,Ravi S Keshari,Tarea Burgett,Florea Lupu,K Mark Coggeshall

Clinical, cosmetic and investigational dermatology 9:167-74 PubMed27524916

2016

Ability of sodium copper chlorophyllin complex to repair photoaged skin by stimulation of biomarkers in human extracellular matrix.

Applications

IHC-P

Species

Human

John P McCook,Thomas J Stephens,Lily I Jiang,Robert M Law,Vincent Gotz

PloS one 11:e0159294 PubMed27428539

2016

Serum Amyloid P Component Ameliorates Neurological Damage Caused by Expressing a Lysozyme Variant in the Central Nervous System of Drosophila melanogaster.

Applications

IHC-P

Species

Unspecified reactive species

Linda Helmfors,Liza Bergkvist,Ann-Christin Brorsson

Proteomics 13:878-92 PubMed23300094

2013

Isolation and identification of mannose-binding proteins and estimation of their abundance in sera from hepatocellular carcinoma patients.

Applications

Unspecified application

Species

Human

Ganglong Yang,Wei Chu,Hua Zhang,Xiuxuan Sun,Tanxi Cai,Liuyi Dang,Qinzhe Wang,Hanjie Yu,Yaogang Zhong,Zhuo Chen,Fuquan Yang,Zheng Li

Journal of leukocyte biology 92:699-711 PubMed22493081

2012

FcγRI mediates serum amyloid P inhibition of fibrocyte differentiation.

Applications

Species

Unspecified reactive species

Jeffrey R Crawford,Darrell Pilling,Richard H Gomer

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24:3416-26 PubMed20448140

2010

A new amyloidosis caused by fibrillar aggregates of mutated corneodesmosin.

Applications

IHC-P

Species

Human

Cécile Caubet,Luc Bousset,Ole Clemmensen,Yannick Sourigues,Anette Bygum,Stéphane Chavanas,Fanny Coudane,Chiung-Yueh Hsu,Regina C Betz,Ronald Melki,Michel Simon,Guy Serre

Science translational medicine 1:5ra13 PubMed20368175

2010

Serum amyloid P inhibits fibrosis through Fc gamma R-dependent monocyte-macrophage regulation in vivo.

Applications

IHC-P

Species

Human

Ana P Castaño,Shuei-Liong Lin,Teresa Surowy,Brian T Nowlin,Swathi A Turlapati,Tejas Patel,Ajay Singh,Shawn Li,Mark L Lupher,Jeremy S Duffield

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Serum Amyloid P/SAP antibody [EP1018Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB45151)

Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB45151)

Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB45151)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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