Rat Recombinant Monoclonal Serum Response Factor SRF antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (AB255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--immunoprecipitation-img12027.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (AB255871)")
![Western blot - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (AB255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--western-blot-img42963.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (AB255871)")
![Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (AB255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--immunoprecipitation-img11917.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (AB255871)")
![Western blot - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (AB255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--western-blot-img8114.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (AB255871)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (AB255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111898.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (AB255871)")
Publications
Filter by
- The Journal of clinical investigation 119:899-9102009Loss of serum response factor in keratinocytes results in hyperproliferative skin disease in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeidi Koegel et. al.PubMed 19307725
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
| Mouse | Tested | Tested | Tested |
| Rat | Expected | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
Alternative names
Serum response factor, SRF
Recommended products
Rat Recombinant Monoclonal Serum Response Factor SRF antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 2C5
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255871 is the carrier-free version of Anti-Serum Response Factor SRF antibody [2C5] ab252868.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Serum Response Factor (SRF) also known as serum response element-binding protein is a transcription factor with a mass of around 67 kDa. SRF regulates gene expression by binding to the serum response element (SRE) found in the promoter region of target genes. It is widely expressed across multiple tissues including muscle brain and epithelial cells. SRF plays a critical role in various cellular functions due to its ability to control the transcription of immediate-early genes in response to a range of extracellular signals.
- Biological function summary
SRF influences cellular processes such as growth differentiation and migration. SRF often functions as a part of a transcriptional complex interacting with co-factors like the ternary complex factor (TCF) from the ETS domain family of transcription factors. This interaction modulates the expression of genes involved in cytoskeletal organization and cell cycle regulation. By doing so SRF coordinates complex cellular activities that are essential for normal development and response to environmental changes.
- Pathways
SRF plays a significant role in the mitogen-activated protein kinase (MAPK) pathway and the RhoA pathway. In the MAPK pathway SRF acts downstream to mediate the response of cells to growth stimuli working closely with MAPK-regulated kinases. Through the RhoA pathway SRF regulates changes in cytoskeletal dynamics and cell movement with the involvement of proteins such as Rho-associated coiled-coil containing protein kinase (ROCK) and myocardin-related transcription factors (MRTFs).
- Associated diseases and disorders
SRF has a strong connection to cardiovascular diseases and certain types of cancer. Alterations in SRF expression or function can contribute to the development of atherosclerosis and myocardial hypertrophy. Additionally dysregulation in SRF activity has been observed in some cancers where it associates with aberrant cell proliferation and migration. In these contexts proteins such as connective tissue growth factor (CTGF) can interact with SRF further impacting disease progression and severity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--immunoprecipitation-img12027.jpg "Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)")
Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)
Serum Response Factor SRF was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with Anti-Serum Response Factor SRF antibody [2C5] ab252868 at 1/30 dilution (2μg in 0.35mg lysates) (36.067μg/ml). Western blot was performed on the immunoprecipitate using Anti-Serum Response Factor SRF antibody [2C5] ab252868 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) was used at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10μg.
Lane 2: Anti-Serum Response Factor SRF antibody [2C5] ab252868 IP in HeLa whole cell lysate.
Lane 3: Rat monoclonal IgG2a (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450) instead of Anti-Serum Response Factor SRF antibody [2C5] ab252868 in HeLa whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590and PMID: 10642500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Serum Response Factor SRF antibody [2C5] ab252868).
All lanes: Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] (Anti-Serum Response Factor SRF antibody [2C5] ab252868)
Predicted band size: 52 kDa
Observed band size: 57 kDa, 67 kDa
![Western blot - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--western-blot-img42963.jpg "Western blot - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)")
Western blot - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 26 seconds; Lane 2: 3 minutes.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590 and PMID: 10642500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Serum Response Factor SRF antibody [2C5] ab252868).
All lanes: Western blot - Anti-Serum Response Factor SRF antibody [2C5] (Anti-Serum Response Factor SRF antibody [2C5] ab252868) at 0.082 µg/mL
Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
Lane 2: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa, 57 kDa, 67 kDa
![Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--immunoprecipitation-img11917.jpg "Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)")
Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)
Serum Response Factor SRF was immunoprecipitated from C2C12 (mouse myoblasts myoblast), whole cell lysate with Anti-Serum Response Factor SRF antibody [2C5] ab252868 at 1/30 dilution (2μg in 0.35mg lysates) (36.067μg/ml). Western blot was performed on the immunoprecipitate using Anti-Serum Response Factor SRF antibody [2C5] ab252868 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) was used at 1/10000 dilution.
Lane 1: C2C12 whole cell lysate 10μg.
Lane 2: Anti-Serum Response Factor SRF antibody [2C5] ab252868 IP in C2C12 whole cell lysate.
Lane 3: Rat monoclonal IgG2a (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450) instead of Anti-Serum Response Factor SRF antibody [2C5] ab252868 in C2C12 whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 6 seconds.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590 and PMID: 10642500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Serum Response Factor SRF antibody [2C5] ab252868).
All lanes: Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] (Anti-Serum Response Factor SRF antibody [2C5] ab252868)
Predicted band size: 52 kDa
Observed band size: 57 kDa, 67 kDa
![Western blot - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--western-blot-img8114.jpg "Western blot - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)")
Western blot - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590 and PMID: 10642500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Serum Response Factor SRF antibody [2C5] ab252868).
All lanes: Western blot - Anti-Serum Response Factor SRF antibody [2C5] (Anti-Serum Response Factor SRF antibody [2C5] ab252868) at 1.082 µg/mL
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg
Lane 2: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg
Lane 3: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg
Lane 4: C2C12 (mouse myoblasts myoblast), whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa, 57 kDa, 67 kDa
Exposure time: 20s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111898.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Serum Response Factor SRF with Anti-Serum Response Factor SRF antibody [2C5] ab252868 at 1/1000 dilution (1.082μg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on mouse cerebrum. The section was incubated with Anti-Serum Response Factor SRF antibody [2C5] ab252868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Serum Response Factor SRF antibody [2C5] ab252868).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54992.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Serum Response Factor SRF with Anti-Serum Response Factor SRF antibody [2C5] ab252868 at 1/1000 dilution (1.082μg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on mouse skeletal muscle tissue. The section was incubated with Anti-Serum Response Factor SRF antibody [2C5] ab252868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Serum Response Factor SRF antibody [2C5] ab252868).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101855.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Serum Response Factor SRF with Anti-Serum Response Factor SRF antibody [2C5] ab252868 at 1/1000 dilution (1.082μg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on rat skeletal muscle tissue. The section was incubated with Anti-Serum Response Factor SRF antibody [2C5] ab252868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Serum Response Factor SRF antibody [2C5] ab252868).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54992.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871), expandable thumbnail](https://content.abcam.com/products/images/serum-response-factor-srf-antibody-2c5-bsa-and-azide-free-ab255871--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101855.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] - BSA and Azide free (ab255871)")
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