AB53147

Anti-Serum Response Factor SRF antibody

Name: Anti-Serum Response Factor SRF antibody (ab53147) | Abcam
Brand: Abcam Limited
SKU: AB53147

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(18 Publications )

Rabbit Polyclonal Serum Response Factor SRF antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human Serum response factor.

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody (AB53147)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody (AB53147)

Immunohistochemical analysis of Serum Response Factor SRF in paraffin embedded human breast carcinoma tissue using ab53147 at a dilution of 1/50. Right hand image : treated with immunising peptide. Left hand image : untreated.

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Western blot - Anti-Serum Response Factor SRF antibody (AB53147)

All lanes:

Western blot - Anti-Serum Response Factor SRF antibody (ab53147) at 1/300 dilution

Lane 1:

Extracts of NIH/3T3 cells treated with PMA (125ng/ml, 30 min) with no peptide

Lane 2:

Extracts of NIH/3T3 cells treated with PMA (125ng/ml, 30 min) with peptide

Predicted band size: 52 kDa

Observed band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Serum response factor. The exact immunogen used to generate this antibody is proprietary information.

P11831

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab53147 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serum Response Factor (SRF) also known as serum response element-binding protein is a transcription factor with a mass of around 67 kDa. SRF regulates gene expression by binding to the serum response element (SRE) found in the promoter region of target genes. It is widely expressed across multiple tissues including muscle brain and epithelial cells. SRF plays a critical role in various cellular functions due to its ability to control the transcription of immediate-early genes in response to a range of extracellular signals.

Biological function summary

SRF influences cellular processes such as growth differentiation and migration. SRF often functions as a part of a transcriptional complex interacting with co-factors like the ternary complex factor (TCF) from the ETS domain family of transcription factors. This interaction modulates the expression of genes involved in cytoskeletal organization and cell cycle regulation. By doing so SRF coordinates complex cellular activities that are essential for normal development and response to environmental changes.

Pathways

SRF plays a significant role in the mitogen-activated protein kinase (MAPK) pathway and the RhoA pathway. In the MAPK pathway SRF acts downstream to mediate the response of cells to growth stimuli working closely with MAPK-regulated kinases. Through the RhoA pathway SRF regulates changes in cytoskeletal dynamics and cell movement with the involvement of proteins such as Rho-associated coiled-coil containing protein kinase (ROCK) and myocardin-related transcription factors (MRTFs).

SRF has a strong connection to cardiovascular diseases and certain types of cancer. Alterations in SRF expression or function can contribute to the development of atherosclerosis and myocardial hypertrophy. Additionally dysregulation in SRF activity has been observed in some cancers where it associates with aberrant cell proliferation and migration. In these contexts proteins such as connective tissue growth factor (CTGF) can interact with SRF further impacting disease progression and severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.

See full target information Serum response factor

Alternative Names

Serum response factor, SRF

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 193:1528-1547 PubMed37422147

2023

Aberrations in Energetic Metabolism and Stress-Related Pathways Contribute to Pathophysiology in the Neb Conditional Knockout Mouse Model of Nemaline Myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca A Slick,Jennifer A Tinklenberg,Jessica Sutton,Liwen Zhang,Hui Meng,Margaret J Beatka,Mark Vanden Avond,Mariah J Prom,Emily Ott,Federica Montanaro,James Heisner,Rafael Toro,Henk Granzier,Aron M Geurts,David F Stowe,R Blake Hill,Michael W Lawlor

The American journal of pathology 193:1548-1567 PubMed37419385

2023

Different Mouse Models of Nemaline Myopathy Harboring Acta1 Mutations Display Differing Abnormalities Related to Mitochondrial Biology.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer A Tinklenberg,Rebecca A Slick,Jessica Sutton,Liwen Zhang,Hui Meng,Margaret J Beatka,Mark Vanden Avond,Mariah J Prom,Emily Ott,Federica Montanaro,James Heisner,Rafael Toro,Edna C Hardeman,Aron M Geurts,David F Stowe,R Blake Hill,Michael W Lawlor

Frontiers in endocrinology 13:1029750 PubMed36568083

2022

Quercetin alleviates diastolic dysfunction and suppresses adverse pro-hypertrophic signaling in diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Linda Bartosova,Csaba Horvath,Peter Galis,Kristina Ferenczyova,Barbora Kalocayova,Adrian Szobi,Adriana Duris-Adameova,Monika Bartekova,Tomas Rajtik

iScience 24:103067 PubMed34541473

2021

A mechanism of cooling hot tumors: Lactate attenuates inflammation in dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hisashi Kanemaru,Yukari Mizukami,Akira Kaneko,Hidemi Tagawa,Toshihiro Kimura,Haruka Kuriyama,Soichiro Sawamura,Ikko Kajihara,Katsunari Makino,Azusa Miyashita,Jun Aoi,Takamitsu Makino,Shinichi Masuguchi,Satoshi Fukushima,Hironobu Ihn

Communications biology 3:576 PubMed33067523

2020

Actomyosin and the MRTF-SRF pathway downregulate FGFR1 in mesenchymal stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jip Zonderland,Silvia Rezzola,Lorenzo Moroni

Journal of molecular endocrinology : PubMed32698141

2020

Long non-coding RNA H19 contributes to wound healing of diabetic foot ulcer.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Li,Yue Zhou,Jing Chen,Tingting Wang,Zhijuan Li,Yili Fu,Changlong Bi,Aixia Zhai

Cellular signalling 73:109674 PubMed32446903

2020

LncRNA XIST modulates 5-hydroxytrytophan-induced visceral hypersensitivity by epigenetic silencing of the SERT gene in mice with diarrhea-predominant IBS.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Hua Zhang,Wen Zhang,Yingjuan Zhang,Wei Wang,Lihong Nie

International journal of molecular medicine 45:497-509 PubMed31894257

2020

lncRNA CYTOR promotes tamoxifen resistance in breast cancer cells via sponging miR‑125a‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Yungyong Liu,Mengdan Li,Huihui Yu,Haozhe Piao

American journal of physiology. Heart and circulat 317:H541-H551 PubMed31298560

2019

Drug-eluting stent specifically designed to target vascular smooth muscle cell phenotypic modulation attenuated restenosis through the YAP pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Huang,Wenwen Zhang,Yuelin Zhu

Experimental cell research 382:111476 PubMed31255599

2019

Role of integrin-linked kinase in the hypoxia-induced phenotypic transition of pulmonary artery smooth muscle cells: Implications for hypoxic pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Jiantong Hou,Bo Liu,Boqian Zhu,Dong Wang,Yong Qiao,Erfei Luo,Abdul Qadir Nawabi,Gaoliang Yan,Chengchun Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Serum Response Factor SRF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody (AB53147)

Western blot - Anti-Serum Response Factor SRF antibody (AB53147)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (18)

Product promise