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AB53130

Anti-Serum Response Factor SRF (phospho S103) antibody

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(5 Publications)

Rabbit Polyclonal Serum Response Factor SRF phospho S103 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human Serum response factor phospho S103 aa 50-150.

View Alternative Names

Serum response factor, SRF

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF (phospho S103) antibody (AB53130)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF (phospho S103) antibody (AB53130)

ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

Western blot - Anti-Serum Response Factor SRF (phospho S103) antibody (AB53130)
  • WB

Unknown

Western blot - Anti-Serum Response Factor SRF (phospho S103) antibody (AB53130)

All lanes:

Western blot - Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130) at 1/300 dilution

Lane 1:

LOVO cell extract treated with serum (20%, 15mins)

Lane 2:

LOVO cell extract treated with serum (20%, 15mins) with immunising peptide

Predicted band size: 52 kDa

Observed band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Serum response factor phospho S103 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P11831

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab53130 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serum Response Factor (SRF) also known as serum response element-binding protein is a transcription factor with a mass of around 67 kDa. SRF regulates gene expression by binding to the serum response element (SRE) found in the promoter region of target genes. It is widely expressed across multiple tissues including muscle brain and epithelial cells. SRF plays a critical role in various cellular functions due to its ability to control the transcription of immediate-early genes in response to a range of extracellular signals.
Biological function summary

SRF influences cellular processes such as growth differentiation and migration. SRF often functions as a part of a transcriptional complex interacting with co-factors like the ternary complex factor (TCF) from the ETS domain family of transcription factors. This interaction modulates the expression of genes involved in cytoskeletal organization and cell cycle regulation. By doing so SRF coordinates complex cellular activities that are essential for normal development and response to environmental changes.

Pathways

SRF plays a significant role in the mitogen-activated protein kinase (MAPK) pathway and the RhoA pathway. In the MAPK pathway SRF acts downstream to mediate the response of cells to growth stimuli working closely with MAPK-regulated kinases. Through the RhoA pathway SRF regulates changes in cytoskeletal dynamics and cell movement with the involvement of proteins such as Rho-associated coiled-coil containing protein kinase (ROCK) and myocardin-related transcription factors (MRTFs).

SRF has a strong connection to cardiovascular diseases and certain types of cancer. Alterations in SRF expression or function can contribute to the development of atherosclerosis and myocardial hypertrophy. Additionally dysregulation in SRF activity has been observed in some cancers where it associates with aberrant cell proliferation and migration. In these contexts proteins such as connective tissue growth factor (CTGF) can interact with SRF further impacting disease progression and severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
See full target information Serum response factor phospho S103
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Acta physiologica (Oxford, England) 220:263-274 PubMed27739650

2016

Striated muscle activator of Rho signalling (STARS) is reduced in ageing human skeletal muscle and targeted by miR-628-5p.

Applications

Unspecified application

Species

Unspecified reactive species

A P Russell,M A Wallace,M Kalanon,E Zacharewicz,P A Della Gatta,A Garnham,S Lamon

Nature neuroscience 17:1720-7 PubMed25347353

2014

Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A Heller,Hannah M Cates,Catherine J Peña,Haosheng Sun,Ningyi Shao,Jian Feng,Sam A Golden,James P Herman,Jessica J Walsh,Michelle Mazei-Robison,Deveroux Ferguson,Scott Knight,Mark A Gerber,Christian Nievera,Ming-Hu Han,Scott J Russo,Carol S Tamminga,Rachael L Neve,Li Shen,H Steve Zhang,Feng Zhang,Eric J Nestler

The Journal of physiology 591:3749-63 PubMed23753523

2013

Effect of resistance exercise contraction mode and protein supplementation on members of the STARS signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kristian Vissing,Stine K Rahbek,Severine Lamon,Jean Farup,Renae J Stefanetti,Marita A Wallace,Mikkel H Vendelbo,Aaron Russell

Proceedings of the National Academy of Sciences of the United States of America 106:316-21 PubMed19116276

2009

Synaptic activity-responsive element in the Arc/Arg3.1 promoter essential for synapse-to-nucleus signaling in activated neurons.

Applications

EMSA

Species

Unspecified reactive species

Takashi Kawashima,Hiroyuki Okuno,Mio Nonaka,Aki Adachi-Morishima,Nan Kyo,Michiko Okamura,Sayaka Takemoto-Kimura,Paul F Worley,Haruhiko Bito

American journal of physiology. Cell physiology 296:C711-23 PubMed19036941

2008

Embryonic stem cell differentiation into smooth muscle cells is mediated by Nox4-produced H2O2.

Applications

Unspecified application

Species

Unspecified reactive species

Qingzhong Xiao,Zhenling Luo,Anna Elena Pepe,Andriani Margariti,Lingfang Zeng,Qingbo Xu
View all publications
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