Rabbit Polyclonal Serum Response Factor SRF phospho S103 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human Serum response factor phospho S103 aa 50-150.
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Publications
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- Acta physiologica (Oxford, England) 220:263-2742016Striated muscle activator of Rho signalling (STARS) is reduced in ageing human skeletal muscle and targeted by miR-628-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA P Russell,M A Wallace,M Kalanon,E Zacharewicz,P A Della Gatta,A Garnham,S LamonPubMed 27739650
- Nature neuroscience 17:1720-72014Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth A Heller et. al.PubMed 25347353
- The Journal of physiology 591:3749-632013Effect of resistance exercise contraction mode and protein supplementation on members of the STARS signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristian Vissing et. al.PubMed 23753523
- Proceedings of the National Academy of Sciences of the United States of America 106:316-212009Synaptic activity-responsive element in the Arc/Arg3.1 promoter essential for synapse-to-nucleus signaling in activated neurons.Applications:EMSAReactive species:Unspecified reactive speciesTakashi Kawashima,Hiroyuki Okuno,Mio Nonaka,Aki Adachi-Morishima,Nan Kyo,Michiko Okamura,Sayaka Takemoto-Kimura,Paul F Worley,Haruhiko BitoPubMed 19116276
- American journal of physiology. Cell physiology 296:C711-232008Embryonic stem cell differentiation into smooth muscle cells is mediated by Nox4-produced H2O2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingzhong Xiao,Zhenling Luo,Anna Elena Pepe,Andriani Margariti,Lingfang Zeng,Qingbo XuPubMed 19036941
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Serum response factor phospho S103 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link P11831
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/300 - 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
Alternative names
Serum response factor, SRF
Recommended products
Rabbit Polyclonal Serum Response Factor SRF phospho S103 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human Serum response factor phospho S103 aa 50-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Serum response factor phospho S103 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link P11831
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab53130 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Serum Response Factor (SRF) also known as serum response element-binding protein is a transcription factor with a mass of around 67 kDa. SRF regulates gene expression by binding to the serum response element (SRE) found in the promoter region of target genes. It is widely expressed across multiple tissues including muscle brain and epithelial cells. SRF plays a critical role in various cellular functions due to its ability to control the transcription of immediate-early genes in response to a range of extracellular signals.
- Biological function summary
SRF influences cellular processes such as growth differentiation and migration. SRF often functions as a part of a transcriptional complex interacting with co-factors like the ternary complex factor (TCF) from the ETS domain family of transcription factors. This interaction modulates the expression of genes involved in cytoskeletal organization and cell cycle regulation. By doing so SRF coordinates complex cellular activities that are essential for normal development and response to environmental changes.
- Pathways
SRF plays a significant role in the mitogen-activated protein kinase (MAPK) pathway and the RhoA pathway. In the MAPK pathway SRF acts downstream to mediate the response of cells to growth stimuli working closely with MAPK-regulated kinases. Through the RhoA pathway SRF regulates changes in cytoskeletal dynamics and cell movement with the involvement of proteins such as Rho-associated coiled-coil containing protein kinase (ROCK) and myocardin-related transcription factors (MRTFs).
- Associated diseases and disorders
SRF has a strong connection to cardiovascular diseases and certain types of cancer. Alterations in SRF expression or function can contribute to the development of atherosclerosis and myocardial hypertrophy. Additionally dysregulation in SRF activity has been observed in some cancers where it associates with aberrant cell proliferation and migration. In these contexts proteins such as connective tissue growth factor (CTGF) can interact with SRF further impacting disease progression and severity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130)
All lanes: Western blot - Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130) at 1/300 dilution
Lane 1: LOVO cell extract treated with serum (20%, 15mins)
Lane 2: LOVO cell extract treated with serum (20%, 15mins) with immunising peptide
Predicted band size: 52 kDa
Observed band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130)
ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
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