AB154825

Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal Seryl-tRNA synthetase/SERS antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

SARS, SERS, SARS1, Seryl-tRNA synthetase, Seryl-tRNA(Ser/Sec) synthetase, SerRS

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/100 dilution (red) or a negative Rabbit IgG (green).

Immunocytochemistry/ Immunofluorescence - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

Immunofluorescence analysis of 293T cells labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

All lanes:

Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Predicted band size: 59 kDa

false

Carrier free

Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10684(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "(Heat to 98°C, allow to cool for 10-20 minutes) Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Seryl-tRNA synthetase also known as SERS is an enzyme responsible for catalyzing the attachment of serine to its corresponding tRNA molecule a process known as aminoacylation. SERS with a molecular mass of approximately 55 kDa finds its expression in various tissues and cell types where it plays a role in protein synthesis. It operates by ensuring that genetic information is translated accurately from nucleic acid sequences to protein sequences important in maintaining the fidelity of protein biosynthesis.

Biological function summary

Through its function of aminoacylating serine to tRNA SERS contributes to the synthesis of proteins by enabling the incorporation of serine residues into polypeptides. SERS does not act alone; instead it operates as a part of the multi-tRNA synthetase complex enhancing its enzymatic efficiency and aiding in the coordination of protein synthesis. Beyond catalysis SERS also participates in cellular response to stress and has non-canonical roles that extend its function beyond tRNA charging.

Pathways

The role of SERS in translation aligns it with pathways involving protein synthesis and amino acid metabolism. One such pathway is the mTOR signaling pathway which SERS influences indirectly through its role in the availability of serine-loaded tRNAs for protein synthesis. Additionally SERS is linked to serine biosynthesis pathways interconnected with proteins like PHGDH which catalyzes the first step in the serine biosynthesis from 3-phosphoglycerate.

The malfunction or aberrant expression of SERS associates with Charcot-Marie-Tooth disease a neuropathy affecting peripheral nerves. Mutations in SERS can disrupt normal nerve function leading to symptoms associated with this disorder. Furthermore SERS links to cancerous processes as its non-canonical functions may influence tumor progression. In cancer SERS interacts with proteins such as oncogenes or tumor suppressors affecting cellular proliferation and survival.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction : serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser) (PubMed : 22353712, PubMed : 24095058, PubMed : 26433229, PubMed : 28236339, PubMed : 34570399, PubMed : 36041817, PubMed : 9431993). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (PubMed : 26433229, PubMed : 28236339, PubMed : 34570399, PubMed : 9431993). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC (PubMed : 24940000). Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA (PubMed : 19423847, PubMed : 19423848, PubMed : 24940000).

See full target information SARS1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 86:103814 PubMed40789258

2025

Inhibition of eIF5A hypusination enhances antioxidant defense to prevent kidney Ischemia/Reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Cougnon,Sébastien Giraud,Maria Materozzi,Elisa Allart,Isabelle Rubera,Claire Mackowiak,Gisèle Jarretou,Nadège Boildieu,Virginie Ameteau,Estelle Lemarié,Hajar Ouahmi,Nicolas Melis,Mallorie Poet,Christophe Duranton,Luc Pellerin,Laurent Counillon,Marina Shkreli,Michel Tauc,Thierry Hauet,Didier F Pisani

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 45:2161-2173 PubMed40536170

2025

Targeting seryl-tRNA synthetase to unlock ischemic resilience in stroke - Insights from a proof-of-concept study.

Applications

Unspecified application

Species

Unspecified reactive species

Miled Bourourou,Nicolas Melis,Marc Cougnon,Gilbert Laffet,Christophe Duranton,Isabelle Rubera,Thomas Maurin,Catherine Heurteaux,Thierry Hauet,Maria Duca,Didier F Pisani,Nicolas Blondeau,Michel Tauc

Annals of neurology 93:244-256 PubMed36088542

2022

Heterozygous Seryl-tRNA Synthetase 1 Variants Cause Charcot-Marie-Tooth Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jin He,Xiao-Xuan Liu,Ming-Ming Ma,Jing-Jing Lin,Jun Fu,Yi-Kun Chen,Guo-Rong Xu,Liu-Qing Xu,Zhi-Fei Fu,Dan Xu,Wen-Feng Chen,Chun-Yan Cao,Yan Shi,Yi-Heng Zeng,Jing Zhang,Xiao-Chun Chen,Ru-Xu Zhang,Ning Wang,Marina Kennerson,Dong-Sheng Fan,Wan-Jin Chen

Journal of agricultural and food chemistry 68:13038-13045 PubMed31597423

2019

ASCT2 Is Involved in SARS-Mediated β-Casein Synthesis of Bovine Mammary Epithelial Cells with Methionine Supply.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Dai,Fengqi Zhao,Jianxin Liu,Hongyun Liu

Journal of dairy science 101:10456-10468 PubMed30219419

2018

Seryl-tRNA synthetase-mediated essential amino acids regulate β-casein synthesis via cell proliferation and mammalian target of rapamycin (mTOR) signaling pathway in bovine mammary epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

W T Dai,R R White,J X Liu,H Y Liu

The Journal of biological chemistry 292:14695-14703 PubMed28655767

2017

Three distinct 3-methylcytidine (mC) methyltransferases modify tRNA and mRNA in mice and humans.

Applications

Unspecified application

Species

Unspecified reactive species

Luang Xu,Xinyu Liu,Na Sheng,Kyaw Soe Oo,Junxin Liang,Yok Hian Chionh,Juan Xu,Fuzhou Ye,Yong-Gui Gao,Peter C Dedon,Xin-Yuan Fu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

Flow Cytometry (Intracellular) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

Immunocytochemistry/ Immunofluorescence - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (AB154825)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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