AB249131

Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Seryl-tRNA synthetase/SERS antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SARS, SERS, SARS1, Seryl-tRNA synthetase, Seryl-tRNA(Ser/Sec) synthetase, SerRS

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free (AB249131)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free (AB249131)

This data was developed using ab154825, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free (AB249131)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free (AB249131)

This data was developed using ab154825, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of 293T cells labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free (AB249131)

This data was developed using ab154825, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free (AB249131)

This data was developed using ab154825, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (<a href='/en-us/products/primary-antibodies/seryl-trna-synthetase-sers-antibody-epr10684b-ab154825'>ab154825</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Predicted band size: 59 kDa

false

Unconjugated

Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10684(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab249131 is the carrier-free version of ab154825.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Seryl-tRNA synthetase also known as SERS is an enzyme responsible for catalyzing the attachment of serine to its corresponding tRNA molecule a process known as aminoacylation. SERS with a molecular mass of approximately 55 kDa finds its expression in various tissues and cell types where it plays a role in protein synthesis. It operates by ensuring that genetic information is translated accurately from nucleic acid sequences to protein sequences important in maintaining the fidelity of protein biosynthesis.

Biological function summary

Through its function of aminoacylating serine to tRNA SERS contributes to the synthesis of proteins by enabling the incorporation of serine residues into polypeptides. SERS does not act alone; instead it operates as a part of the multi-tRNA synthetase complex enhancing its enzymatic efficiency and aiding in the coordination of protein synthesis. Beyond catalysis SERS also participates in cellular response to stress and has non-canonical roles that extend its function beyond tRNA charging.

Pathways

The role of SERS in translation aligns it with pathways involving protein synthesis and amino acid metabolism. One such pathway is the mTOR signaling pathway which SERS influences indirectly through its role in the availability of serine-loaded tRNAs for protein synthesis. Additionally SERS is linked to serine biosynthesis pathways interconnected with proteins like PHGDH which catalyzes the first step in the serine biosynthesis from 3-phosphoglycerate.

The malfunction or aberrant expression of SERS associates with Charcot-Marie-Tooth disease a neuropathy affecting peripheral nerves. Mutations in SERS can disrupt normal nerve function leading to symptoms associated with this disorder. Furthermore SERS links to cancerous processes as its non-canonical functions may influence tumor progression. In cancer SERS interacts with proteins such as oncogenes or tumor suppressors affecting cellular proliferation and survival.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction : serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser) (PubMed : 22353712, PubMed : 24095058, PubMed : 26433229, PubMed : 28236339, PubMed : 34570399, PubMed : 36041817, PubMed : 9431993). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (PubMed : 26433229, PubMed : 28236339, PubMed : 34570399, PubMed : 9431993). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC (PubMed : 24940000). Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA (PubMed : 19423847, PubMed : 19423848, PubMed : 24940000).

See full target information SARS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free (AB249131)

Immunocytochemistry/ Immunofluorescence - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free (AB249131)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free (AB249131)

Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] - BSA and Azide free (AB249131)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise