AB250642

Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Seryl-tRNA synthetase/SERS antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

SARS, SERS, SARS1, Seryl-tRNA synthetase, Seryl-tRNA(Ser/Sec) synthetase, SerRS

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] - BSA and Azide free (AB250642)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] - BSA and Azide free (AB250642)

This data was developed using ab183025, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Seryl-tRNA synthetase/SERS with ab183025 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] - BSA and Azide free (AB250642)

This data was developed using ab183025, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Human K562 cell lysate immunoprecipitated using ab183025 at 1/50 dilution (lane 1) or a negative control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (<a href='/en-us/products/primary-antibodies/seryl-trna-synthetase-sers-antibody-epr10685-ab183025'>ab183025</a>)

Predicted band size: 59 kDa

false

Supplier Data

Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] - BSA and Azide free (AB250642)

This data was developed using ab183025, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (<a href='/en-us/products/primary-antibodies/seryl-trna-synthetase-sers-antibody-epr10685-ab183025'>ab183025</a>) at 1/20000 dilution

Lane 1:

HT29 cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 59 kDa

false

Supplier Data

Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] - BSA and Azide free (AB250642)

This data was developed using ab183025, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (<a href='/en-us/products/primary-antibodies/seryl-trna-synthetase-sers-antibody-epr10685-ab183025'>ab183025</a>) at 1/5000 dilution

Lane 1:

PC12 cell lysate at 10 µg

Lane 2:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 59 kDa

false

Unconjugated

Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10685

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab250642 is the carrier-free version of ab183025.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Seryl-tRNA synthetase also known as SERS is an enzyme responsible for catalyzing the attachment of serine to its corresponding tRNA molecule a process known as aminoacylation. SERS with a molecular mass of approximately 55 kDa finds its expression in various tissues and cell types where it plays a role in protein synthesis. It operates by ensuring that genetic information is translated accurately from nucleic acid sequences to protein sequences important in maintaining the fidelity of protein biosynthesis.

Biological function summary

Through its function of aminoacylating serine to tRNA SERS contributes to the synthesis of proteins by enabling the incorporation of serine residues into polypeptides. SERS does not act alone; instead it operates as a part of the multi-tRNA synthetase complex enhancing its enzymatic efficiency and aiding in the coordination of protein synthesis. Beyond catalysis SERS also participates in cellular response to stress and has non-canonical roles that extend its function beyond tRNA charging.

Pathways

The role of SERS in translation aligns it with pathways involving protein synthesis and amino acid metabolism. One such pathway is the mTOR signaling pathway which SERS influences indirectly through its role in the availability of serine-loaded tRNAs for protein synthesis. Additionally SERS is linked to serine biosynthesis pathways interconnected with proteins like PHGDH which catalyzes the first step in the serine biosynthesis from 3-phosphoglycerate.

The malfunction or aberrant expression of SERS associates with Charcot-Marie-Tooth disease a neuropathy affecting peripheral nerves. Mutations in SERS can disrupt normal nerve function leading to symptoms associated with this disorder. Furthermore SERS links to cancerous processes as its non-canonical functions may influence tumor progression. In cancer SERS interacts with proteins such as oncogenes or tumor suppressors affecting cellular proliferation and survival.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction : serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser) (PubMed : 22353712, PubMed : 24095058, PubMed : 26433229, PubMed : 28236339, PubMed : 34570399, PubMed : 36041817, PubMed : 9431993). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (PubMed : 26433229, PubMed : 28236339, PubMed : 34570399, PubMed : 9431993). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC (PubMed : 24940000). Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA (PubMed : 19423847, PubMed : 19423848, PubMed : 24940000).

See full target information SARS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neuroreport 33:451-462 PubMed35775321

2022

Notoginsenoside R1 alleviates spinal cord injury by inhibiting oxidative stress, neuronal apoptosis, and inflammation via activating the nuclear factor erythroid 2 related factor 2/heme oxygenase-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbo Luo,Zhangli Bao,Mingjian Zhou,Yuxin Chen,Zhaoxi Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] - BSA and Azide free (AB250642)

Immunoprecipitation - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] - BSA and Azide free (AB250642)

Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] - BSA and Azide free (AB250642)

Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] - BSA and Azide free (AB250642)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise