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Rabbit Recombinant Monoclonal SESN1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

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Images

Western blot - Anti-SESN1 antibody [EPR1930(2)] (AB134091), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] (AB134091), expandable thumbnail
  • Western blot - Anti-SESN1 antibody [EPR1930(2)] (AB134091), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] (AB134091), expandable thumbnail
  • OI-RD Scanning - Anti-SESN1 antibody [EPR1930(2)] (AB134091), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Tested
Not recommended
Expected
Rat
Not recommended
Not recommended
Tested
Not recommended
Expected

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:25263562, PubMed:26449471). This stress-inducible metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085). Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed:15105503). However, this could not be confirmed (By similarity).

Alternative names

PA26, SEST1, SESN1, Sestrin-1, p53-regulated protein PA26

Recommended products

Rabbit Recombinant Monoclonal SESN1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1930(2)
Purification technique
Affinity purification Protein A
Dissociation constant
2.08 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SESN1 also known as Sestrin-1 is a protein that has significant significance in cellular stress responses. It has a molecular weight of approximately 57 kDa. This protein mainly expresses in tissues such as liver skin and heart. Sestrin-1 acts as a regulator of cellular homeostasis modulating factors tied to oxidative stress correction. The protein carries antioxidant properties mediating the regeneration of peroxiredoxins which are important for detoxifying peroxides within cells.

Biological function summary

The Sestrin-1 protein plays a role in the cellular defense against oxidative damage. It incorporates into complexes that participate in inhibiting the activity of the mechanistic target of rapamycin complex 1 (mTORC1) a critical regulator of cell growth and metabolism. Sestrin-1 achieves this by activating AMP-activated protein kinase (AMPK) which serves as an energy sensor in the cell. Additionally it can suppress free radical accumulation during periods of cellular stress helping to prevent damage to DNA and proteins.

Pathways

Sestrin-1 is a vital component of the signaling pathways that involve the AMPK and mTOR pathways. It interacts actively with proteins such as TSC2 in mTOR signaling and LKB1 in the AMPK pathway. Through these pathways Sestrin-1 influences the regulation of protein synthesis and autophagy. It acts as a link between energy status and cellular growth control underpinning its involvement in adaptive cellular responses to environmental stresses.

Associated diseases and disorders

Sestrin-1 involvement connects to conditions like cancer and neurodegenerative diseases. In cancer its role in inhibiting the mTORC1 pathway gives it potential anti-tumorigenic properties as overactive mTOR signaling can lead to uncontrolled cell proliferation. Additionally in neurodegenerative disorders Sestrin-1 reduces stress-induced neuronal damage through its antioxidant functions. Sestrin-1 interacts with proteins like p53 a well-known tumor suppressor to aid in cellular protective mechanisms providing a link between its protective role and disease suppression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-SESN1 antibody [EPR1930(2)] (ab134091), expandable thumbnail

    Western blot - Anti-SESN1 antibody [EPR1930(2)] (ab134091)

    The bands observed are consistent with what has been described in PMID: 29751091 and PMID: 29263116

    All lanes: Western blot - Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution

    Lane 1: Human heart lysates at 15 µg

    Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

    Lane 3: F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysates at 15 µg

    Lane 4: C2C12 (Mouse myoblasts myoblast) whole cell lysates at 15 µg

    Lane 5: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

    Lane 6: Rat heart lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa

    Observed band size: 65 kDa

  • Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] (ab134091), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] (ab134091)

    Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells treated with 20 U/ml IFN alpha 1 for 24 hours (Red) / Untreated control (Green) labelling Anti-SESN1 with unpurified Anti-MX1 antibody [EPR19967] ab207414 at 1/600 dilution.Cells were fixed with 2% paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Black - isotype control, Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue -unlabelled control,cells without incubation with primary and secondary antibodies.

  • Western blot - Anti-SESN1 antibody [EPR1930(2)] (ab134091), expandable thumbnail

    Western blot - Anti-SESN1 antibody [EPR1930(2)] (ab134091)

    All lanes: Western blot - Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution

    Lane 1: Ramos cell lysate at 10 µg

    Lane 2: K562 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: C2C12 cell lysate at 10 µg

    Lane 5: NIH3T3 cell lysate at 10 µg

    Lane 6: F9 cell lysate at 10 µg

    Lane 7: JAR cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 57 kDa

  • Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] (ab134091), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] (ab134091)

    Intracellular Flow Cytometry analysis of permeabilized K562 cells labeling SESN1 with unpurified ab134091 at 1/100 dilution (red). Rabbit IgG (negative control) shown in green.

  • OI-RD Scanning - Anti-SESN1 antibody [EPR1930(2)] (ab134091), expandable thumbnail

    OI-RD Scanning - Anti-SESN1 antibody [EPR1930(2)] (ab134091)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

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