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AB134091

Anti-SESN1 antibody [EPR1930(2)]

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(11 Publications)

Rabbit Recombinant Monoclonal SESN1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

View Alternative Names

PA26, SEST1, SESN1, Sestrin-1, p53-regulated protein PA26

5 Images
Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] (AB134091)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] (AB134091)

Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells treated with 20 U/ml IFN alpha 1 for 24 hours (Red) / Untreated control (Green) labelling Anti-SESN1 with unpurified ab207414 at 1/600 dilution.Cells were fixed with 2% paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Black - isotype control, Rabbit monoclonal IgG (ab172730). Blue -unlabelled control,cells without incubation with primary and secondary antibodies.

Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] (AB134091)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] (AB134091)

Intracellular Flow Cytometry analysis of permeabilized K562 cells labeling SESN1 with unpurified ab134091 at 1/100 dilution (red). Rabbit IgG (negative control) shown in green.

Western blot - Anti-SESN1 antibody [EPR1930(2)] (AB134091)
  • WB

Lab

Western blot - Anti-SESN1 antibody [EPR1930(2)] (AB134091)

The bands observed are consistent with what has been described in PMID : 29751091 and PMID : 29263116

All lanes:

Western blot - Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution

Lane 1:

Human heart lysates at 15 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

Lane 3:

F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysates at 15 µg

Lane 4:

C2C12 (Mouse myoblasts myoblast) whole cell lysates at 15 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

Lane 6:

Rat heart lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 65 kDa

false

Western blot - Anti-SESN1 antibody [EPR1930(2)] (AB134091)
  • WB

Unknown

Western blot - Anti-SESN1 antibody [EPR1930(2)] (AB134091)

All lanes:

Western blot - Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution

Lane 1:

Ramos cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

C2C12 cell lysate at 10 µg

Lane 5:

NIH3T3 cell lysate at 10 µg

Lane 6:

F9 cell lysate at 10 µg

Lane 7:

JAR cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 57 kDa

false

OI-RD Scanning - Anti-SESN1 antibody [EPR1930(2)] (AB134091)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SESN1 antibody [EPR1930(2)] (AB134091)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-SESN1 antibody [EPR1930(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1930(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SESN1 also known as Sestrin-1 is a protein that has significant significance in cellular stress responses. It has a molecular weight of approximately 57 kDa. This protein mainly expresses in tissues such as liver skin and heart. Sestrin-1 acts as a regulator of cellular homeostasis modulating factors tied to oxidative stress correction. The protein carries antioxidant properties mediating the regeneration of peroxiredoxins which are important for detoxifying peroxides within cells.
Biological function summary

The Sestrin-1 protein plays a role in the cellular defense against oxidative damage. It incorporates into complexes that participate in inhibiting the activity of the mechanistic target of rapamycin complex 1 (mTORC1) a critical regulator of cell growth and metabolism. Sestrin-1 achieves this by activating AMP-activated protein kinase (AMPK) which serves as an energy sensor in the cell. Additionally it can suppress free radical accumulation during periods of cellular stress helping to prevent damage to DNA and proteins.

Pathways

Sestrin-1 is a vital component of the signaling pathways that involve the AMPK and mTOR pathways. It interacts actively with proteins such as TSC2 in mTOR signaling and LKB1 in the AMPK pathway. Through these pathways Sestrin-1 influences the regulation of protein synthesis and autophagy. It acts as a link between energy status and cellular growth control underpinning its involvement in adaptive cellular responses to environmental stresses.

Sestrin-1 involvement connects to conditions like cancer and neurodegenerative diseases. In cancer its role in inhibiting the mTORC1 pathway gives it potential anti-tumorigenic properties as overactive mTOR signaling can lead to uncontrolled cell proliferation. Additionally in neurodegenerative disorders Sestrin-1 reduces stress-induced neuronal damage through its antioxidant functions. Sestrin-1 interacts with proteins like p53 a well-known tumor suppressor to aid in cellular protective mechanisms providing a link between its protective role and disease suppression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed : 25263562, PubMed : 26449471). This stress-inducible metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed : 23274085). Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed : 15105503). However, this could not be confirmed (By similarity).
See full target information SESN1
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 32:219-231 PubMed36413176

2022

Effects of ferroptosis in myocardial ischemia/reperfusion model of rat and its association with Sestrin 1.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Yang,Wei Wang,Yiling Zhang,Jifei Nong,Longdan Zhang

Nature cell biology 24:1461-1474 PubMed36109671

2022

An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory.

Applications

Unspecified application

Species

Unspecified reactive species

Alessio Lanna,Bruno Vaz,Clara D'Ambra,Salvatore Valvo,Claudia Vuotto,Valerio Chiurchiù,Oliver Devine,Massimo Sanchez,Giovanna Borsellino,Arne N Akbar,Marco De Bardi,Derek W Gilroy,Michael L Dustin,Brendan Blumer,Michael Karin

Experimental gerontology 155:111581 PubMed34634412

2021

Aerobic exercise inhibits inflammatory response in atherosclerosis via Sestrin1 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfeng Sun,Yawei Wu,Yingping Jiang,Hao Liu

Scientific reports 11:19163 PubMed34580402

2021

Sarcopenia-derived exosomal micro-RNA 16-5p disturbs cardio-repair via a pro-apoptotic mechanism in myocardial infarction in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Taiki Hayasaka,Naofumi Takehara,Tatsuya Aonuma,Kohei Kano,Kiwamu Horiuchi,Naoki Nakagawa,Hiroki Tanaka,Jun-Ichi Kawabe,Naoyuki Hasebe

Nature communications 11:3148 PubMed32561715

2020

Leucine regulates autophagy via acetylation of the mTORC1 component raptor.

Applications

Unspecified application

Species

Unspecified reactive species

Sung Min Son,So Jung Park,Eleanna Stamatakou,Mariella Vicinanza,Fiona M Menzies,David C Rubinsztein

European journal of immunology 50:1154-1166 PubMed32297666

2020

Sestrin1 inhibits oxidized low-density lipoprotein-induced activation of NLRP3 inflammasome in macrophages in a murine atherosclerosis model.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Keping,Sun Yunfeng,Xiao Pengzhuo,Li Liang,Xu Chenhong,Mao Jinghua

International immunology 32:421-432 PubMed32154559

2020

Sestrin3 enhances macrophage-mediated generation of T helper 1 and T helper 17 cells in a mouse colitis model.

Applications

Unspecified application

Species

Unspecified reactive species

Liuqing Ge,Min Xu,Steven R Brant,Shaoping Liu,Chengliang Zhu,Jian Shang,Qiu Zhao,Feng Zhou

Oncotarget 8:90132-90143 PubMed29163816

2017

Sestrin2 and sestrin3 suppress NK-92 cell-mediated cytotoxic activity on ovarian cancer cells through AMPK and mTORC1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejin Wang,Weifeng Liu,Deyi Zhuang,Shaoxian Hong,Jingfang Chen

Oncotarget 8:30908-30921 PubMed28427210

2017

Dependence of p53-deficient cells on the DHX9 DExH-box helicase.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Lee,Jerry Pelletier

Journal of cellular and molecular medicine 21:1193-1205 PubMed28181410

2017

Sestrin 1 ameliorates cardiac hypertrophy via autophagy activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ruicong Xue,Junyi Zeng,Yili Chen,Cong Chen,Weiping Tan,Jingjing Zhao,Bin Dong,Yu Sun,Yugang Dong,Chen Liu
View all publications
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Product promise

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