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AB244361

Anti-SESN2/Sestrin-2 antibody

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Rabbit Polyclonal SESN2/Sestrin-2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SESN2 aa 200-350.

View Alternative Names

Hi95, SEST2, SESN2, Sestrin-2, Hypoxia-induced gene

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SESN2/Sestrin-2 antibody (AB244361)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SESN2/Sestrin-2 antibody (AB244361)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling SESN2/Sestrin-2 using ab244361 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SESN2/Sestrin-2 antibody (AB244361)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SESN2/Sestrin-2 antibody (AB244361)

Formalin-fixed, paraffin-embedded human colon tissue stained for SESN2/Sestrin-2 with ab244361 at a 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SESN2 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

P58004

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SESN2 also known as Sestrin-2 or Hi95 is a stress-inducible protein that plays a role in cellular responses to oxidative stress. It has a molecular mass of approximately 60 kDa and is expressed in various tissues including the liver heart and muscle. Sestrin-2 acts as an antioxidant protein that helps reduce reactive oxygen species (ROS) and maintains cellular redox balance. It contains domains that directly interact with target proteins to regulate cellular activity enabling cells to respond efficiently to stress signals.
Biological function summary

Sestrin-2 regulates cell survival and homeostasis during oxidative stress conditions. It modulates the activity of key signaling pathways that control autophagy and cell growth. Sestrin-2 does not function alone but interacts with other proteins to form functional complexes. This allows it to fine-tune cellular processes and contributes to its role in promoting cell repair mechanisms. Its induction during stress conditions demonstrates its engagement in promoting cellular adaptation and resistance to damage.

Pathways

Sestrin-2 engages in signaling networks such as the AMPK and mTOR pathways. Its interaction with AMP-activated protein kinase (AMPK) triggers energy homeostasis and metabolic stress responses. It also negatively regulates the mammalian target of rapamycin (mTOR) to support autophagy and inhibit cell growth when necessary. Sestrin-2 therefore plays an important role in coordinating responses between these pathways ensuring optimal cellular function during periods of environmental stress or nutrient deprivation.

SESN2 is implicated in conditions related to oxidative stress and metabolic dysregulation such as cancer and type 2 diabetes. Its involvement in cancer is connected to its regulatory role on the mTOR pathway often overactive in tumor growth. The protein’s modulation of AMPK-mTOR signaling can impact cancer cell survival and response to treatment. Additionally in diabetes Sestrin-2 plays a part in maintaining insulin sensitivity and glucose metabolism often disrupted in diabetic patients. Its ability to regulate oxidative stress and cellular metabolism draws a link between Sestrin-2 and these complex disorders emphasizing its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as an intracellular leucine sensor that negatively regulates the mTORC1 signaling pathway through the GATOR complex (PubMed : 18692468, PubMed : 25263562, PubMed : 25457612, PubMed : 26449471, PubMed : 26586190, PubMed : 26612684, PubMed : 31586034, PubMed : 35114100, PubMed : 35831510, PubMed : 36528027). In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents mTORC1 signaling (PubMed : 18692468, PubMed : 25263562, PubMed : 25457612, PubMed : 26449471, PubMed : 26586190, PubMed : 26612684, PubMed : 31586034, PubMed : 35114100, PubMed : 35831510, PubMed : 36528027). Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed : 26449471, PubMed : 26586190, PubMed : 35114100, PubMed : 35831510, PubMed : 36528027). This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via mTORC1 (PubMed : 24947615). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed : 23274085). May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed : 18692468). Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (PubMed : 26612684). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed : 15105503). However, this could not be confirmed (PubMed : 19113821).
See full target information SESN2
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