Rabbit Polyclonal SESN3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SESN3 aa 1-300.
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- Journal of experimental & clinical cancer research 39:1692020CircRNA hsa_circ_0002577 accelerates endometrial cancer progression through activating IGF1R/PI3K/Akt pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Wang et. al.PubMed 32847606
- Nature communications 11:1902020Sestrins are evolutionarily conserved mediators of exercise benefits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMyungjin Kim et. al.PubMed 31929512
- Oncogene 39:1080-10972019A drug library screen identifies Carbenoxolone as novel FOXO inhibitor that overcomes FOXO3-mediated chemoprotection in high-stage neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefan Salcher,Gilles Spoden,Judith Hagenbuchner,Sebastian Führer,Teresa Kaserer,Martin Tollinger,Petra Huber-Cantonati,Thomas Gruber,Daniela Schuster,Ronald Gust,Heinz Zwierzina,Thomas Müller,Ursula Kiechl-Kohlendorfer,Michael J Ausserlechner,Petra ObexerPubMed 31591479
- American journal of physiology. Heart and circulat 317:H39-H482019Cardioprotective roles of sestrin 1 and sestrin 2 against doxorubicin cardiotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuiting Li et. al.PubMed 31026186
- Experimental gerontology 110:23-342018Sestrins are differentially expressed with age in the skeletal muscle of men: A cross-sectional analysis.Applications:WBReactive species:Unspecified reactive speciesNina Zeng,Randall F D'Souza,Cameron J Mitchell,David Cameron-SmithPubMed 29751091
- Physiological reports 5:2017Acute resistance exercise induces Sestrin2 phosphorylation and p62 dephosphorylation in human skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNina Zeng,Randall F D'Souza,Vandre C Figueiredo,James F Markworth,Llion A Roberts,Jonathan M Peake,Cameron J Mitchell,David Cameron-SmithPubMed 29263116
- Oncotarget 8:90132-901432017Sestrin2 and sestrin3 suppress NK-92 cell-mediated cytotoxic activity on ovarian cancer cells through AMPK and mTORC1 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuejin Wang et. al.PubMed 29163816
- Nature communications 5:42332014Hepatoprotective role of Sestrin2 against chronic ER stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHwan-Woo Park et. al.PubMed 24947615
- American journal of physiology. Gastrointestinal a 307:G58-652014The inhibitory effect of ethanol on Sestrin3 in the pathogenesis of ethanol-induced liver injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinqin Kang et. al.PubMed 24833709
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SESN3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P58005
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
May function as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway (PubMed:25263562). May also regulate the insulin-receptor signaling pathway through activation of TORC2 (By similarity). This metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity).
Alternative names
SEST3, SESN3, Sestrin-3
Recommended products
Rabbit Polyclonal SESN3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SESN3 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SESN3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P58005
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SESN3 also known as Sestrin 3 is a protein involved in cellular stress response with a mass of approximately 57 kDa. This protein works as an antioxidant and helps in regulating the cellular redox state. SESN3 is expressed in various tissues most prominently in metabolically active tissues such as the liver and muscle. It responds to oxidative stress by influencing antioxidant defense mechanisms and also maintains cellular homeostasis.
- Biological function summary
SESN3 plays an important role in metabolic regulation and cellular protection. It activates AMP-activated protein kinase (AMPK) contributing to the suppression of the mammalian target of rapamycin complex 1 (mTORC1) signaling. SESN3 does not exist as a part of a larger protein complex itself but interacts with other proteins to exert its functions. Through these interactions SESN3 helps manage cellular metabolism preventing damage from oxidative stress.
- Pathways
SESN3 participates in the PI3K/AKT/mTOR and AMPK signaling pathways both essential for regulating cell growth and energy metabolism. It interacts with proteins such as mTOR and TSC2 (tuberous sclerosis complex 2) which are necessary for balancing cellular growth and nutrient availability. SESN3 modulates these pathways by inhibiting mTORC1 activity impacting processes like protein synthesis and autophagy.
- Associated diseases and disorders
SESN3 has associations with metabolic disorders and cancer. Dysregulation of SESN3 affects pathways like AMPK and mTOR linked to type 2 diabetes and obesity. Additionally changes in SESN3 levels influence tumor growth as SESN3 affects processes involved in cell survival and proliferation. SESN3's interaction with AMPK and mTOR links it to cancerous processes highlighting its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-SESN3 antibody (ab97792)
7.5% SDS-PAGE
Signal was developed with Trident ECL plus-Enhanced.
All lanes: Western blot - Anti-SESN3 antibody (ab97792) at 1/500 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg
Lane 2: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Lane 3: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg
Lane 4: C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 57 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SESN3 antibody (ab97792)
Immunofluorescence analysis of paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cell labeling SESN3 with ab97792 at a 1/200 dilution. The lower image was merged with DNA probe.
Western blot - Anti-SESN3 antibody (ab97792)
7.5% SDS-PAGE
All lanes: Western blot - Anti-SESN3 antibody (ab97792) at 1/1000 dilution
All lanes: Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 57 kDa
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