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AB97792

Anti-SESN3 antibody

2

(1 Review)

|

(9 Publications)

Rabbit Polyclonal SESN3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SESN3 aa 1-300.

View Alternative Names

SEST3, SESN3, Sestrin-3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SESN3 antibody (AB97792)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SESN3 antibody (AB97792)

Immunofluorescence analysis of paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cell labeling SESN3 with ab97792 at a 1/200 dilution. The lower image was merged with DNA probe.

Western blot - Anti-SESN3 antibody (AB97792)
  • WB

Supplier Data

Western blot - Anti-SESN3 antibody (AB97792)

7.5% SDS-PAGE

Signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-SESN3 antibody (ab97792) at 1/500 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 3:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Lane 4:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57 kDa

false

Western blot - Anti-SESN3 antibody (AB97792)
  • WB

Supplier Data

Western blot - Anti-SESN3 antibody (AB97792)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-SESN3 antibody (ab97792) at 1/1000 dilution

All lanes:

Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SESN3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P58005

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Complementary Products

Primary Antibodies

AB134091

Anti-SESN1 antibody [EPR1930(2)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] (AB134091)

  • 0
  • 0
Primary Antibodies

AB178518

Anti-SESN2/Sestrin-2 antibody [EPR18907]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-SESN2/Sestrin-2 antibody [EPR18907] (AB178518)

  • 0
  • 0
Primary Antibodies

AB26

Anti-p53 antibody [PAb 240]

KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [PAb 240] (AB26)

  • 4
  • 56
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SESN3 also known as Sestrin 3 is a protein involved in cellular stress response with a mass of approximately 57 kDa. This protein works as an antioxidant and helps in regulating the cellular redox state. SESN3 is expressed in various tissues most prominently in metabolically active tissues such as the liver and muscle. It responds to oxidative stress by influencing antioxidant defense mechanisms and also maintains cellular homeostasis.
Biological function summary

SESN3 plays an important role in metabolic regulation and cellular protection. It activates AMP-activated protein kinase (AMPK) contributing to the suppression of the mammalian target of rapamycin complex 1 (mTORC1) signaling. SESN3 does not exist as a part of a larger protein complex itself but interacts with other proteins to exert its functions. Through these interactions SESN3 helps manage cellular metabolism preventing damage from oxidative stress.

Pathways

SESN3 participates in the PI3K/AKT/mTOR and AMPK signaling pathways both essential for regulating cell growth and energy metabolism. It interacts with proteins such as mTOR and TSC2 (tuberous sclerosis complex 2) which are necessary for balancing cellular growth and nutrient availability. SESN3 modulates these pathways by inhibiting mTORC1 activity impacting processes like protein synthesis and autophagy.

SESN3 has associations with metabolic disorders and cancer. Dysregulation of SESN3 affects pathways like AMPK and mTOR linked to type 2 diabetes and obesity. Additionally changes in SESN3 levels influence tumor growth as SESN3 affects processes involved in cell survival and proliferation. SESN3's interaction with AMPK and mTOR links it to cancerous processes highlighting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway (PubMed : 25263562). May also regulate the insulin-receptor signaling pathway through activation of TORC2 (By similarity). This metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity).
See full target information SESN3
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research 39:169 PubMed32847606

2020

CircRNA hsa_circ_0002577 accelerates endometrial cancer progression through activating IGF1R/PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Lili Yin,Xiaofei Sun

Nature communications 11:190 PubMed31929512

2020

Sestrins are evolutionarily conserved mediators of exercise benefits.

Applications

Unspecified application

Species

Unspecified reactive species

Myungjin Kim,Alyson Sujkowski,Sim Namkoong,Bondong Gu,Tyler Cobb,Boyoung Kim,Allison H Kowalsky,Chun-Seok Cho,Ian Semple,Seung-Hyun Ro,Carol Davis,Susan V Brooks,Michael Karin,Robert J Wessells,Jun Hee Lee

Oncogene 39:1080-1097 PubMed31591479

2019

A drug library screen identifies Carbenoxolone as novel FOXO inhibitor that overcomes FOXO3-mediated chemoprotection in high-stage neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Salcher,Gilles Spoden,Judith Hagenbuchner,Sebastian Führer,Teresa Kaserer,Martin Tollinger,Petra Huber-Cantonati,Thomas Gruber,Daniela Schuster,Ronald Gust,Heinz Zwierzina,Thomas Müller,Ursula Kiechl-Kohlendorfer,Michael J Ausserlechner,Petra Obexer

American journal of physiology. Heart and circulat 317:H39-H48 PubMed31026186

2019

Cardioprotective roles of sestrin 1 and sestrin 2 against doxorubicin cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiting Li,Yin Huang,Ian Semple,Myungjin Kim,Zunjian Zhang,Jun Hee Lee

Experimental gerontology 110:23-34 PubMed29751091

2018

Sestrins are differentially expressed with age in the skeletal muscle of men: A cross-sectional analysis.

Applications

WB

Species

Unspecified reactive species

Nina Zeng,Randall F D'Souza,Cameron J Mitchell,David Cameron-Smith

Physiological reports 5: PubMed29263116

2017

Acute resistance exercise induces Sestrin2 phosphorylation and p62 dephosphorylation in human skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Zeng,Randall F D'Souza,Vandre C Figueiredo,James F Markworth,Llion A Roberts,Jonathan M Peake,Cameron J Mitchell,David Cameron-Smith

Oncotarget 8:90132-90143 PubMed29163816

2017

Sestrin2 and sestrin3 suppress NK-92 cell-mediated cytotoxic activity on ovarian cancer cells through AMPK and mTORC1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejin Wang,Weifeng Liu,Deyi Zhuang,Shaoxian Hong,Jingfang Chen

Nature communications 5:4233 PubMed24947615

2014

Hepatoprotective role of Sestrin2 against chronic ER stress.

Applications

Unspecified application

Species

Unspecified reactive species

Hwan-Woo Park,Haeli Park,Seung-Hyun Ro,Insook Jang,Ian A Semple,David N Kim,Myungjin Kim,Myeongjin Nam,Deqiang Zhang,Lei Yin,Jun Hee Lee

American journal of physiology. Gastrointestinal a 307:G58-65 PubMed24833709

2014

The inhibitory effect of ethanol on Sestrin3 in the pathogenesis of ethanol-induced liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xinqin Kang,Kateryna Petyaykina,Rongya Tao,Xiwen Xiong,X Charlie Dong,Suthat Liangpunsakul
View all publications
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Product promise

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