Rabbit Polyclonal SET/TAF-I antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SET aa 100-200.
Images
Publications
Filter by
- Molecular medicine reports 13:4329-342016Construction of SET overexpression vector and its effects on the proliferation and apoptosis of 293T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Wang et. al.PubMed 27035430
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SET aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q01105
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.1 µg/mL | Notes - |
Species Human | Dilution info 0.1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
Alternative names
Protein SET, HLA-DR-associated protein II, Inhibitor of granzyme A-activated DNase, PHAPII, Phosphatase 2A inhibitor I2PP2A, Template-activating factor I, IGAAD, I-2PP2A, TAF-I, SET
Recommended products
Rabbit Polyclonal SET/TAF-I antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SET aa 100-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SET aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q01105
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SET/TAF-I also known as Inhibitor of Protein Phosphatase 2A (I2PP2A) is a multifunctional protein with an approximate mass of 39 kDa. It is ubiquitously expressed in various human tissues including the brain liver and testis. SET/TAF-I plays a role in chromatin remodeling and gene transcription regulation by inhibiting protein phosphatase 2A (PP2A) activity. The protein interacts with nucleosome assembly and in chromatin sliding affecting the overall structure and function of chromosomal material.
- Biological function summary
In the context of cellular processes SET/TAF-I functions as a histone chaperone and is a part of the nucleosome assembly complex. This protein is involved in nucleosome disassembly and assembly during DNA replication and repair and it modulates transcriptional access for various transcription factors. It regulates the acetylation of histones influencing gene expression levels. The SET/TAF-I also participates in apoptosis and autophagy demonstrating its versatility in cellular control mechanisms.
- Pathways
Studies show that SET/TAF-I engages in the MAPK/ERK signaling pathway which is critical for cell cycle progression and proliferation. It is linked with other proteins such as HMGN proteins and histone deacetylases playing a substantial role in chromatin dynamics and genomic stability. Furthermore SET/TAF-I is central to the signaling pathway that maintains normal cellular functions and responds to stress signals like oxidative stress demonstrating its importance in normal cell regulation.
- Associated diseases and disorders
Abnormalities in SET/TAF-I levels link to Alzheimer's disease and acute myeloid leukemia. In Alzheimer's the protein accumulation leads to tau hyperphosphorylation an important feature of neurodegeneration influencing PP2A's role in phosphatase activity. In acute myeloid leukemia SET/TAF-I dysregulation results in altered cell proliferation and survival signaling implicating proteins like BCL-2 and downstream apoptotic regulators. This demonstrates the protein's involvement in both neurological disorders and malignancies highlighting its broader clinical significance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Western blot - Anti-SET/TAF-I antibody (ab92872)
Detection: Chemiluminescence.Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-SET/TAF-I antibody (ab92872) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HEK293T whole cell lysate at 50 µg
Lane 3: Mouse NIH3T3 whole cell lysate at 50 µg
Predicted band size: 33 kDa
Exposure time: 30s
Immunoprecipitation - Anti-SET/TAF-I antibody (ab92872)
3μg ab92872 were used to immunoprecipitate SET/TAF-I from 1mg HeLa whole cell lysate. 20% IP was loaded and probed with ab92872 at 1μg/ml.
All lanes: Immunoprecipitation - Anti-SET/TAF-I antibody (ab92872)
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody (ab92872)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue, labeling SET/TAF-I with ab92872 at 1/1000 (0.2 μg/mL) dilution. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody (ab92872)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue, labeling SET/TAF-I with ab92872 at 1/1000 (0.2 μg/mL) dilution. Detection: DAB.
This image is courtesy of an anonymous customer reviewWestern blot - Anti-SET/TAF-I antibody (ab92872)
Blocking Step: 5% Milk for 30 minutes at room temperature.
ab92872 recognises both isoforms of human SET/TAF-I.All lanes: Western blot - Anti-SET/TAF-I antibody (ab92872) at 1/2000 dilution
All lanes: Lysate of the soluble fraction of HeLa or U20S cells at 30 µg
SecondaryAll lanes: An HRP polymer Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: ~37 kDa, ~40 kDa
Exposure time: 1min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com