Rabbit Recombinant Monoclonal SET/TAF-I antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat samples. Cited in 9 publications.
Images
![Western blot - Anti-SET/TAF-I antibody [EPR12973] (AB181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--western-blot-img103666.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SET/TAF-I antibody [EPR12973] (AB181990)")
![Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (AB181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--immunocytochemistry-immunofluorescence-img14898.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (AB181990)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (AB181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114189.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (AB181990)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (AB181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86634.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (AB181990)")
![Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] (AB181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--immunoprecipitation-img784.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] (AB181990)")
Publications
Filter by
- Nucleic acids research 50:6687-67012022Genome-wide CRISPR/Cas9 transcriptional activation screen identifies a histone acetyltransferase inhibitor complex as a regulator of HIV-1 integration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiong Zhang,Shaobo Wang,Wanyu Li,Edwin Yau,Hui Hui,Parmit Kumar Singh,Vasudevan Achuthan,Maile Ann Young Karris,Alan N Engelman,Tariq M RanaPubMed 35713529
- Nature communications 12:72972021Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Ting Yen,May Chien,Pei-Yi Wu,Chi-Chang Ho,Chun-Te Ho,Kevin Chih-Yang Huang,Shu-Fen Chiang,K S Clifford Chao,William Tzu-Liang Chen,Shih-Chieh HungPubMed 34911954
- Nature communications 12:34972021Site-specific ubiquitylation acts as a regulator of linker histone H1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva Höllmüller,Simon Geigges,Marie L Niedermeier,Kai-Michael Kammer,Simon M Kienle,Daniel Rösner,Martin Scheffner,Andreas Marx,Florian StengelPubMed 34108453
- Journal of cell science 133:2020Histone H1 eviction by the histone chaperone SET reduces cell survival following DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesImke K Mandemaker et. al.PubMed 32184266
- Experimental and therapeutic medicine 18:3801-38082019TAF-Iβ deficiency inhibits proliferation and promotes apoptosis by rescuing PP2A and inhibiting the AKT/GSK-3β pathway in leukemic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanfeng Liu et. al.PubMed 31611934
- Chemosphere 231:151-1602019A pilot study on polycystic ovarian syndrome caused by neonatal exposure to tributyltin and bisphenol A in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhibing Yang et. al.PubMed 31129395
- Cell 175:1492-1506.e192018A Membraneless Organelle Associated with the Endoplasmic Reticulum Enables 3'UTR-Mediated Protein-Protein Interactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeirui Ma et. al.PubMed 30449617
- International journal of molecular medicine 40:1869-18802017Rivaroxaban attenuates thrombosis by targeting the NF-κB signaling pathway in a rat model of deep venous thrombus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunhao Ma et. al.PubMed 29039441
- Nature 522:363-72015Alternative 3' UTRs act as scaffolds to regulate membrane protein localization.Applications:Flow Cyt, WBReactive species:Unspecified reactive speciesBinyamin D Berkovits et. al.PubMed 25896326
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Expected | Expected | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/100000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
Alternative names
Protein SET, HLA-DR-associated protein II, Inhibitor of granzyme A-activated DNase, PHAPII, Phosphatase 2A inhibitor I2PP2A, Template-activating factor I, IGAAD, I-2PP2A, TAF-I, SET
Recommended products
Rabbit Recombinant Monoclonal SET/TAF-I antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12973
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SET/TAF-I also known as Inhibitor of Protein Phosphatase 2A (I2PP2A) is a multifunctional protein with an approximate mass of 39 kDa. It is ubiquitously expressed in various human tissues including the brain liver and testis. SET/TAF-I plays a role in chromatin remodeling and gene transcription regulation by inhibiting protein phosphatase 2A (PP2A) activity. The protein interacts with nucleosome assembly and in chromatin sliding affecting the overall structure and function of chromosomal material.
- Biological function summary
In the context of cellular processes SET/TAF-I functions as a histone chaperone and is a part of the nucleosome assembly complex. This protein is involved in nucleosome disassembly and assembly during DNA replication and repair and it modulates transcriptional access for various transcription factors. It regulates the acetylation of histones influencing gene expression levels. The SET/TAF-I also participates in apoptosis and autophagy demonstrating its versatility in cellular control mechanisms.
- Pathways
Studies show that SET/TAF-I engages in the MAPK/ERK signaling pathway which is critical for cell cycle progression and proliferation. It is linked with other proteins such as HMGN proteins and histone deacetylases playing a substantial role in chromatin dynamics and genomic stability. Furthermore SET/TAF-I is central to the signaling pathway that maintains normal cellular functions and responds to stress signals like oxidative stress demonstrating its importance in normal cell regulation.
- Associated diseases and disorders
Abnormalities in SET/TAF-I levels link to Alzheimer's disease and acute myeloid leukemia. In Alzheimer's the protein accumulation leads to tau hyperphosphorylation an important feature of neurodegeneration influencing PP2A's role in phosphatase activity. In acute myeloid leukemia SET/TAF-I dysregulation results in altered cell proliferation and survival signaling implicating proteins like BCL-2 and downstream apoptotic regulators. This demonstrates the protein's involvement in both neurological disorders and malignancies highlighting its broader clinical significance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-SET/TAF-I antibody [EPR12973] (ab181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--western-blot-img103666.jpg "Western blot - Anti-SET/TAF-I antibody [EPR12973] (ab181990)")
Western blot - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
Blocking/dilution buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-SET/TAF-I antibody [EPR12973] (ab181990) at 1/100000 dilution
Lane 1: Jurkat cell lysate at 20 µg
Lane 2: 293T cell lysate at 20 µg
Lane 3: Hela cell lysate at 20 µg
Lane 4: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--immunocytochemistry-immunofluorescence-img14898.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)")
Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling SET/TAF-I with Purified ab181990 at 1/500 dilution (5 μg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114189.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
Immunohistochemical analysis of paraffin-embedded Human nephroblastoma tissue labeling SET/TAF-I with ab181990 at a 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86634.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling SET/TAF-I with ab181990 at a 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] (ab181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--immunoprecipitation-img784.jpg "Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] (ab181990)")
Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
Immunoprecipitation of Jurkat cell lysate labeling SET/TAF-I using ab181990 at a 1/30 dilution (lane 1). A goat anti-rabbit IgG, (H+L), peroxidase conjugated secondary antibody was used at a dilution of 1/1000. Blocking/dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
Predicted band size: 33 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--immunocytochemistry-immunofluorescence-img13505.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)")
Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cell line staining SET/TAF-I using ab181990 at 1/1000 dilution. A Dylight 555 conjugated Goat anti rabbit IgG at 1/200 dilution was used for secondary antibody detection. Counter stain: Dapi.
![Flow Cytometry (Intracellular) - Anti-SET/TAF-I antibody [EPR12973] (ab181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--flow-cytometry-intracellular-img83028.jpg "Flow Cytometry (Intracellular) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)")
Flow Cytometry (Intracellular) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Hela cells labeling SET/TAF-I using ab181990at a 1/10 dilution, or a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--immunocytochemistry-immunofluorescence-img13505.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)")
![Flow Cytometry (Intracellular) - Anti-SET/TAF-I antibody [EPR12973] (ab181990), expandable thumbnail](https://content.abcam.com/products/images/set-taf-i-antibody-epr12973-ab181990--flow-cytometry-intracellular-img83028.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com