Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free (AB235387)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Hela cells labeling SET/TAF-I using ab181990at a 1/10 dilution, or a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181990).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free (AB235387)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling SET/TAF-I with Purified ab181990 at 1/500 dilution (5 μg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181990).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free (AB235387)

Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cell line staining SET/TAF-I using ab181990 at 1/1000 dilution. A Dylight 555 conjugated Goat anti rabbit IgG at 1/200 dilution was used for secondary antibody detection. Counter stain : Dapi.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181990).

• IP

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Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free (AB235387)

Immunoprecipitation of Jurkat cell lysate labeling SET/TAF-I using ab181990 at a 1/30 dilution (lane 1). A goat anti-rabbit IgG, (H+L), peroxidase conjugated secondary antibody was used at a dilution of 1/1000. Blocking/dilution buffer and concentration : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181990).

All lanes:

Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] (<a href='/en-us/products/primary-antibodies/set-taf-i-antibody-epr12973-ab181990'>ab181990</a>)

Predicted band size: 33 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free (AB235387)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling SET/TAF-I with ab181990 at a 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain : Hematoxylin

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181990).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.