AB202437

Anti-SET5 antibody [EPR20102]

RabMAb
Recombinant
KO Validated
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal SET5 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication.

View Alternative Names

YHR207C, SET5, Histone-lysine N-methyltransferase SET5, SET domain-containing protein 5

2 Images

Supplier Data

Western blot - Anti-SET5 antibody [EPR20102] (AB202437)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SET5 antibody [EPR20102] (ab202437) at 1/5000 dilution

All lanes:

Saccharomyces cerevisiae whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

true

Exposure time: 3s

Supplier Data

Western blot - Anti-SET5 antibody [EPR20102] (AB202437)

Blocking/Dilution buffer : 5% NFDM/TBST.

Set5 KO strain (Y02901) was purchased from Euroscarf.

All lanes:

Western blot - Anti-SET5 antibody [EPR20102] (ab202437) at 1/5000 dilution

Lane 1:

Saccharomyces cerevisiae (WT) whole cell lysate at 10 µg

Lane 2:

Saccharomyces cerevisiae (SET5 KO) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

true

Exposure time: 30s

Carrier free

Anti-SET5 antibody [EPR20102] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20102

Isotype

IgG

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SET5 also known as lysine methyltransferase 5 (KMT5) is a histone methyltransferase enzyme with a role in epigenetic regulation. It weighs approximately 46 kDa and localizes predominantly in the nucleus. SET5 selectively methylates histone H4 at specific lysine residues which affects chromatin structure and gene expression. The expression of SET5 occurs in diverse tissues with notable activity in cells undergoing high rates of division.

Biological function summary

SET5 influences gene expression by modulating chromatin accessibility. This methyltransferase enzyme adds methyl groups to histone proteins leading to changes in chromatin compaction and transcriptional activity. SET5 does not form part of a larger protein complex but directly interacts with histone substrates to exert its effects. Its activity links to cellular processes that drive proliferation and differentiation.

Pathways

SET5 integrates into epigenetic signaling that govern transcription. It contributes to the regulation of the MAPK signaling pathway influencing cell growth and differentiation. SET5 activity also interfaces with the mitogen-activated protein kinase (MAPK) pathway where its function may interact with proteins like ERK2 in the context of gene regulation. Through these interactions SET5 plays a role in fine-tuning the cellular response to external and internal cues intended for growth and proliferation.

Alterations in SET5 function associate with cancer and developmental disorders. In cancers aberrant SET5 activity may lead to uncontrolled cellular proliferation through dysregulated transcription of key growth-related genes. Disorders such as Kabuki syndrome have connections to dysregulation in histone methylation machinery with SET5 possibly linking with MLL2 another methyltransferase implicated in the disorder. Understanding SET5's role in these conditions helps guide potential therapeutic approaches targeting chromatin modifiers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone methyltransferase that monomethylates 'Lys-5', 'Lys-8' and 'Lys-12' of histone H4 (H4K5me1, H4K8me1 and H4K12me1, respectively), thereby controlling gene expression and remodeling chromatin structures (PubMed : 22343720). Functions together with the chromatin-modifying complexes COMPASS and NuA4 to regulate cell growth and stress responses (PubMed : 22343720).. (Microbial infection) Acts as a virus host factor involved in the replication of positive-strand RNA viruses like the MBV.

See full target information SET5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Prostate 80:1145-1156 PubMed32659025

2020

CXCL12γ induces human prostate and mammary gland development.

Applications

Unspecified application

Species

Unspecified reactive species

Younghun Jung,Jin Koo Kim,Eunsohl Lee,Frank C Cackowski,Ann M Decker,Paul H Krebsbach,Russell S Taichman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SET5 antibody [EPR20102]

Western blot - Anti-SET5 antibody [EPR20102] (AB202437)

Western blot - Anti-SET5 antibody [EPR20102] (AB202437)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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