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Rabbit Recombinant Monoclonal SET7 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

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Images

Western blot - Anti-SET7 antibody [EPR5574] (AB124708), expandable thumbnail
  • Western blot - Anti-SET7 antibody [EPR5574] (AB124708), expandable thumbnail
  • Western blot - Anti-SET7 antibody [EPR5574] (AB124708), expandable thumbnail
  • Immunoprecipitation - Anti-SET7 antibody [EPR5574] (AB124708), expandable thumbnail
  • Western blot - Anti-SET7 antibody [EPR5574] (AB124708), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/120
Notes

For unpurified use at 1/10 - 1/100.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed:11779497, PubMed:11850410, PubMed:12540855, PubMed:12588998, PubMed:16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:12540855, PubMed:12588998, PubMed:16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed:12588998, PubMed:16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed:16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed:15099517, PubMed:15525938, PubMed:16415881, PubMed:35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed:15099517, PubMed:16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed:15525938, PubMed:16415881, PubMed:17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity).

Alternative names

KIAA1717, KMT7, SET7, SET9, SETD7, Histone-lysine N-methyltransferase SETD7, Histone H3-K4 methyltransferase SETD7, Lysine N-methyltransferase 7, SET domain-containing protein 7, SET7/9, H3-K4-HMTase SETD7

Recommended products

Rabbit Recombinant Monoclonal SET7 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5574
Purification technique
Affinity purification Protein A
Dissociation constant
6.93 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SET7 also known as SETD7 or KMT7 is a lysine methyltransferase that adds methyl groups to lysine residues on target proteins influencing their function and stability. This protein has a molecular mass of approximately 55 kDa. SET7 is expressed in a variety of tissues including the heart liver and pancreas suggesting its versatile role across different biological systems.

Biological function summary

SET7 contributes to gene regulation by methylating histone H3 at lysine 4 (H3K4) a mark generally associated with transcriptional activation. It also methylates non-histone proteins such as p53 TAF10 and STAT3 affecting their activity and interactions. SET7 operates distinctively and does not need to be part of any complex to fulfill its function effectively in diverse cellular processes.

Pathways

SET7 modulates transcriptional pathways such as the p53 signaling pathway and the NF-kB pathway. It interacts with the p53 protein affecting cell cycle regulation and the response to DNA damage. The modification of NF-kB by SET7 can influence inflammation and immune responses. Through these pathways SET7 plays a role in maintaining cellular homeostasis and response to stress.

Associated diseases and disorders

SET7 is involved in conditions like cancer and cardiovascular diseases. Its methylation of p53 links it to tumor suppression activities while its impact on NF-kB relates to inflammatory responses. Dysregulation of SET7 activity can lead to abnormal cell proliferation and inflammation pointing to its potential as a target for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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7 product images

  • Western blot - Anti-SET7 antibody [EPR5574] (ab124708), expandable thumbnail

    Western blot - Anti-SET7 antibody [EPR5574] (ab124708)

    Lanes 1-3: Merged signal (red and green). Green - ab124708 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab124708 Anti-SET7 antibody [EPR5574] was shown to specifically react with SET7 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human SETD7 (SET7) knockout HeLa cell line ab265770 (knockout cell lysate Human SETD7 (SET7) knockout HeLa cell lysate ab257668) was used. Wild-type and SET7 knockout samples were subjected to SDS-PAGE. ab124708 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SET7 antibody [EPR5574] (ab124708) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: SETD7 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human SETD7 (SET7) knockout HeLa cell line (Human SETD7 (SET7) knockout HeLa cell line ab265770)

    Lane 3: U-2 OS cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 41 kDa

    Observed band size: 50 kDa

  • Western blot - Anti-SET7 antibody [EPR5574] (ab124708), expandable thumbnail

    Western blot - Anti-SET7 antibody [EPR5574] (ab124708)

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-SET7 antibody [EPR5574] (ab124708) at 1/5000 dilution

    Lane 1: PC-12 cell lysate at 20 µg

    Lane 2: NIH/3T3 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 41 kDa

  • Western blot - Anti-SET7 antibody [EPR5574] (ab124708), expandable thumbnail

    Western blot - Anti-SET7 antibody [EPR5574] (ab124708)

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-SET7 antibody [EPR5574] (ab124708) at 1/5000 dilution

    All lanes: MCF-7 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 41 kDa

  • Immunoprecipitation - Anti-SET7 antibody [EPR5574] (ab124708), expandable thumbnail

    Immunoprecipitation - Anti-SET7 antibody [EPR5574] (ab124708)

    ab124708 (purified) at 1/120 immunoprecipitating SET7 in Jurkat whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used intead of Anti-Rab11A antibody [EPR7587(B)] ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-SET7 antibody [EPR5574] (ab124708)

    Predicted band size: 41 kDa

  • Western blot - Anti-SET7 antibody [EPR5574] (ab124708), expandable thumbnail

    Western blot - Anti-SET7 antibody [EPR5574] (ab124708)

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-SET7 antibody [EPR5574] (ab124708) at 1/2000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 41 kDa

  • Western blot - Anti-SET7 antibody [EPR5574] (ab124708), expandable thumbnail

    Western blot - Anti-SET7 antibody [EPR5574] (ab124708)

    All lanes: Western blot - Anti-SET7 antibody [EPR5574] (ab124708) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: BxPC-3 cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Lane 4: MCF-7 cell lysate at 10 µg

    Lane 5: C6 cell lysate at 10 µg

    Lane 6: RAW264.7 cell lysate at 10 µg

    Lane 7: PC-12 cell lysate at 10 µg

    Lane 8: NIH/3T3 cell lysate at 10 µg

    Predicted band size: 41 kDa

  • OI-RD Scanning - Anti-SET7 antibody [EPR5574] (ab124708), expandable thumbnail

    OI-RD Scanning - Anti-SET7 antibody [EPR5574] (ab124708)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

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