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AB300480

Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free)

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Rabbit Recombinant Monoclonal SETD1B antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Mouse, Human, Rat samples.

View Alternative Names

KIAA1076, KMT2G, SET1B, SETD1B, Histone-lysine N-methyltransferase SETD1B, Lysine N-methyltransferase 2G, SET domain-containing protein 1B, hSET1B

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SETD1B with ab300479 at 1/100 (5.2 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human colon at a low level (PMID : 24925220). The section was incubated with ab300479 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Flow Cytometry (Intracellular) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling SETD1B with ab300479 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling SETD1B with ab300479 at 1/100 (5.2 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human colon cancer (PMID : 24925220). The section was incubated with ab300479 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunocytochemistry/ Immunofluorescence - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling SETD1B with ab300479 at 1/100 (5.2 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Immunocytochemistry/ Immunofluorescence - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling SETD1B with ab300479 at 1/100 (5.2 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in F9 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver cancer tissue labeling SETD1B with ab300479 at 1/100 (5.2 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on mouse liver cancer. The section was incubated with ab300479 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Flow Cytometry (Intracellular) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling SETD1B with ab300479 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)
  • WB

Lab

Western blot - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 29138278).

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-SETD1B antibody [EPR25142-11] (<a href='/en-us/products/primary-antibodies/setd1b-antibody-epr25142-11-ab300479'>ab300479</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting SETD1B, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 209 kDa

false

Exposure time: 3min

Western blot - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)
  • WB

Lab

Western blot - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.

Exposure time : Lane 1 - 3 minutes, Lane 2 : 26 seconds.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 29138278).

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

An unknown band at ~115 kDa was detected in lane 3.

All lanes:

Western blot - Anti-SETD1B antibody [EPR25142-11] (<a href='/en-us/products/primary-antibodies/setd1b-antibody-epr25142-11-ab300479'>ab300479</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 209 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25142-11

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SETD1B also known as KMT2G is a histone methyltransferase protein with a molecular mass of approximately 200 kDa. It functions mechanically by specifically methylating lysine 4 on histone H3 (H3K4) a critical action for chromatin modification. This methylation regulates gene transcription and the enzyme predominantly exerts its effects in the nucleus. SETD1B expresses across various tissues which suggests a broad regulatory role in different cellular environments.
Biological function summary

The role of SETD1B extends to functioning as part of the SET1/MLL complex which is responsible for the mono- di- and tri-methylation of histone H3 at lysine 4 (H3K4). The complex plays a significant role in transcriptional regulation influencing gene activation and silencing across different cellular processes. SETD1B's activity modulates the expression of genes involved in the regulation of development differentiation and cellular proliferation impacting numerous biological outcomes.

Pathways

The involvement of SETD1B in gene expression regulation positions it notably in the Wnt signaling pathway. This pathway is important for controlling cell fate and proliferation. Furthermore SETD1B interacts with other proteins like β-catenin enhancing transcription of Wnt target genes. Additionally SETD1B takes part in the regulation of pathways involving chromatin remodeling associating closely with proteins such as ASH2L which affects histone methylation states.

Alterations in SETD1B are linked to intellectual disability and autism spectrum disorders. Disruptions in the protein's methylation function can lead to improper gene expression patterns emphasizing its association with neurodevelopmental conditions. SETD1B also connects with the CBP protein in certain cancer pathways where abnormal histone modification can drive oncogenesis. These interactions highlight SETD1B's significance in maintaining regular cellular function and its potential impact when mutant or dysregulated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism (PubMed : 17355966, PubMed : 25561738). Part of chromatin remodeling machinery, forms H3K4me1, H3K4me2 and H3K4me3 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed : 17355966, PubMed : 25561738). Plays an essential role in regulating the transcriptional programming of multipotent hematopoietic progenitor cells and lymphoid lineage specification during hematopoiesis (By similarity).
See full target information SETD1B
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Product promise

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For full details, please see our Terms & Conditions

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