Rabbit Recombinant Monoclonal SETD1B antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Mouse, Human, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
---|---|---|---|---|---|
Human | Not recommended | Tested | Tested | Tested | Tested |
Mouse | Not recommended | Tested | Tested | Tested | Tested |
Rat | Not recommended | Expected | Expected | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Species Human | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism (PubMed:17355966, PubMed:25561738). Part of chromatin remodeling machinery, forms H3K4me1, H3K4me2 and H3K4me3 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed:17355966, PubMed:25561738). Plays an essential role in regulating the transcriptional programming of multipotent hematopoietic progenitor cells and lymphoid lineage specification during hematopoiesis (By similarity).
KIAA1076, KMT2G, SET1B, KIAA1076, KMT2G, SET1B, SETD1B, Histone-lysine N-methyltransferase SETD1B, Lysine N-methyltransferase 2G, SET domain-containing protein 1B, hSET1B
Rabbit Recombinant Monoclonal SETD1B antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Mouse, Human, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR25142-11
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
SETD1B also known as KMT2G is a histone methyltransferase protein with a molecular mass of approximately 200 kDa. It functions mechanically by specifically methylating lysine 4 on histone H3 (H3K4) a critical action for chromatin modification. This methylation regulates gene transcription and the enzyme predominantly exerts its effects in the nucleus. SETD1B expresses across various tissues which suggests a broad regulatory role in different cellular environments.
The role of SETD1B extends to functioning as part of the SET1/MLL complex which is responsible for the mono- di- and tri-methylation of histone H3 at lysine 4 (H3K4). The complex plays a significant role in transcriptional regulation influencing gene activation and silencing across different cellular processes. SETD1B's activity modulates the expression of genes involved in the regulation of development differentiation and cellular proliferation impacting numerous biological outcomes.
The involvement of SETD1B in gene expression regulation positions it notably in the Wnt signaling pathway. This pathway is important for controlling cell fate and proliferation. Furthermore SETD1B interacts with other proteins like β-catenin enhancing transcription of Wnt target genes. Additionally SETD1B takes part in the regulation of pathways involving chromatin remodeling associating closely with proteins such as ASH2L which affects histone methylation states.
Alterations in SETD1B are linked to intellectual disability and autism spectrum disorders. Disruptions in the protein's methylation function can lead to improper gene expression patterns emphasizing its association with neurodevelopmental conditions. SETD1B also connects with the CBP protein in certain cancer pathways where abnormal histone modification can drive oncogenesis. These interactions highlight SETD1B's significance in maintaining regular cellular function and its potential impact when mutant or dysregulated.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
This data was developed using Anti-SETD1B antibody [EPR25142-11] ab300479, the same antibody clone in a different buffer formulation.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29138278).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
All lanes: Western blot - Anti-SETD1B antibody [EPR25142-11] (Anti-SETD1B antibody [EPR25142-11] ab300479) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg
Lane 2: HeLa transfected with siRNA specifically targeting SETD1B, whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 209 kDa
Exposure time: 3min
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29138278).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
This data was developed using Anti-SETD1B antibody [EPR25142-11] ab300479, the same antibody clone in a different buffer formulation.
Exposure time: Lane 1 - 3 minutes, Lane 2: 26 seconds.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29138278).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
An unknown band at ~115 kDa was detected in lane 3.
All lanes: Western blot - Anti-SETD1B antibody [EPR25142-11] (Anti-SETD1B antibody [EPR25142-11] ab300479) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 209 kDa
Exposure time: Lane 1 - 3 minutes, Lane 2: 26 seconds.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29138278).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
An unknown band at ~115 kDa was detected in lane 3.
This data was developed using Anti-SETD1B antibody [EPR25142-11] ab300479, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling SETD1B with Anti-SETD1B antibody [EPR25142-11] ab300479 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
This data was developed using Anti-SETD1B antibody [EPR25142-11] ab300479, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling SETD1B with Anti-SETD1B antibody [EPR25142-11] ab300479 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
This data was developed using Anti-SETD1B antibody [EPR25142-11] ab300479, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling SETD1B with Anti-SETD1B antibody [EPR25142-11] ab300479 at 1/100 (5.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in F9 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
This data was developed using Anti-SETD1B antibody [EPR25142-11] ab300479, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling SETD1B with Anti-SETD1B antibody [EPR25142-11] ab300479 at 1/100 (5.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in HeLa cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
This data was developed using Anti-SETD1B antibody [EPR25142-11] ab300479, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver cancer tissue labeling SETD1B with Anti-SETD1B antibody [EPR25142-11] ab300479 at 1/100 (5.2 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on mouse liver cancer. The section was incubated with Anti-SETD1B antibody [EPR25142-11] ab300479 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
This data was developed using Anti-SETD1B antibody [EPR25142-11] ab300479, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SETD1B with Anti-SETD1B antibody [EPR25142-11] ab300479 at 1/100 (5.2 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human colon at a low level (PMID: 24925220). The section was incubated with Anti-SETD1B antibody [EPR25142-11] ab300479 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
This data was developed using Anti-SETD1B antibody [EPR25142-11] ab300479, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling SETD1B with Anti-SETD1B antibody [EPR25142-11] ab300479 at 1/100 (5.2 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human colon cancer (PMID: 24925220). The section was incubated with Anti-SETD1B antibody [EPR25142-11] ab300479 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
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