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AB317724

Anti-SETD7 antibody [RAB-C220]

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Human Recombinant Monoclonal SET7 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SETD7 aa 1 to C-terminus.

View Alternative Names

KIAA1717, KMT7, SET7, SET9, SETD7, Histone-lysine N-methyltransferase SETD7, Histone H3-K4 methyltransferase SETD7, Lysine N-methyltransferase 7, SET domain-containing protein 7, SET7/9, H3-K4-HMTase SETD7

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SETD7 antibody [RAB-C220] (AB317724)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SETD7 antibody [RAB-C220] (AB317724)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of ab317724 (1 : 100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1 : 1500 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom primary antibody/secondary, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.

Flow Cytometry - Anti-SETD7 antibody [RAB-C220] (AB317724)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-SETD7 antibody [RAB-C220] (AB317724)

Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of ab317724 (blue line) at a dilution of 1 : 100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1 : 1000.

Western blot - Anti-SETD7 antibody [RAB-C220] (AB317724)
  • WB

Supplier Data

Western blot - Anti-SETD7 antibody [RAB-C220] (AB317724)

Chimeric rabbit version of ab317724 was used for this WB. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Lane 1:

Western blot - Anti-SETD7 antibody [RAB-C220] (ab317724) at 0.01 µg/mL

Lane 2:

Western blot - Anti-SETD7 antibody [RAB-C220] (ab317724) at 0.1 µg/mL

Lane 3:

Western blot - Anti-SETD7 antibody [RAB-C220] (ab317724) at 0.03 µg/mL

Lane 1:

Nuclear lysate of MCF7 (35ug protein in RIPA buffer)

Lane 2:

Nuclear lysate of Jurkat (35ug protein in RIPA buffer)

Lane 3:

Nuclear lysate of A549 (35ug protein in RIPA buffer)

Predicted band size: 41 kDa

false

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

RAB-C220

Isotype

Unknown

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SETD7 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8WTS6

Specificity

This antibody recognizes SETD7 (Histone-lysine N-methyltransferase SETD7). It binds to a folded domain, amino acids 2-366.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified by Immobilized Metal Affinity Chromatography.
Storage buffer
pH: 7.2 Preservative: 0.02% Proclin 300 Constituents: 99.98% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SET7 also known as SETD7 or KMT7 is a lysine methyltransferase that adds methyl groups to lysine residues on target proteins influencing their function and stability. This protein has a molecular mass of approximately 55 kDa. SET7 is expressed in a variety of tissues including the heart liver and pancreas suggesting its versatile role across different biological systems.
Biological function summary

SET7 contributes to gene regulation by methylating histone H3 at lysine 4 (H3K4) a mark generally associated with transcriptional activation. It also methylates non-histone proteins such as p53 TAF10 and STAT3 affecting their activity and interactions. SET7 operates distinctively and does not need to be part of any complex to fulfill its function effectively in diverse cellular processes.

Pathways

SET7 modulates transcriptional pathways such as the p53 signaling pathway and the NF-kB pathway. It interacts with the p53 protein affecting cell cycle regulation and the response to DNA damage. The modification of NF-kB by SET7 can influence inflammation and immune responses. Through these pathways SET7 plays a role in maintaining cellular homeostasis and response to stress.

SET7 is involved in conditions like cancer and cardiovascular diseases. Its methylation of p53 links it to tumor suppression activities while its impact on NF-kB relates to inflammatory responses. Dysregulation of SET7 activity can lead to abnormal cell proliferation and inflammation pointing to its potential as a target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed : 11779497, PubMed : 11850410, PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed : 12588998, PubMed : 16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed : 16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed : 15099517, PubMed : 15525938, PubMed : 16415881, PubMed : 35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed : 15099517, PubMed : 16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed : 15525938, PubMed : 16415881, PubMed : 17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity).
See full target information SETD7
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